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- W3093805280 endingPage "115863" @default.
- W3093805280 startingPage "115863" @default.
- W3093805280 abstract "Cigarette smoke (CS) affects immune functions, leading to severe outcomes in smokers. Robust evidence addresses the immunotoxic effects of combustible tobacco products. As heat-not-burn tobacco products (HNBT) vaporize lower levels of combustible products, we here compared the effects of cigarette smoke (CS) and HNBT vapor on Jurkat T cells. Cells were exposed to air, conventional cigarettes or heatsticks of HNBT for 30 min and were stimulated or not with phorbol myristate acetate (PMA). Cell viability, proliferation, reactive oxygen species (ROS) production, 8-OHdG, MAP-kinases and nuclear factor κB (NFκB) activation and metallothionein expression (MTs) were assessed by flow cytometry; nitric oxide (NO) and cytokine levels were measured by Griess reaction and ELISA, respectively. Levels of metals in the exposure chambers were quantified by inductively coupled plasma mass spectrometry. MT expressions were quantified by immunohistochemistry in the lungs and liver of C57Bl/6 mice exposed to CS, HNBT or air (1 h, twice a day for five days: via inhalation). While both CS and HBNT exposures increased cell death, CS led to a higher number of necrotic cells, increased the production of ROS, NO, inflammatory cytokines and MTs when compared to HNBT-exposed cells, and led to a higher expression of MTs in mice. CS released higher amounts of metals. CS and HNBT exposures decreased PMA-induced interleukin-2 (IL-2) secretion and impaired Jurkat proliferation, effects also seen in cells exposed to nicotine. Although HNBT vapor does not activate T cells as CS does, exposure to both HNBT and CS suppressed proliferation and IL-2 release, a pivotal cytokine involved with T cell proliferation and tolerance, and this effect may be related to nicotine content in both products. • CS exposure led to higher incidence of late-apoptosis and necrosis than HNBT. • Only CS exposure caused DNA damage, oxidative stress and cytokines secretion by Jurkat cell. • Only CS exposure caused significant in vivo and in vitro metallothioneins expression. • CS, HNBT vapor or nicotine exposures impaired interleukin-2 secretion and Jurkat T cell proliferation. • The toxicity caused by HNBT vapor exposure is related to nicotine harmful actions. CS exposure displayed more severe toxic effects in Jurkat T cells than HNBT vapor; nevertheless, such CS and HNBT impaired IL-2 secretion and cell proliferation, which may be due to nicotine actions." @default.
- W3093805280 created "2020-10-29" @default.
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- W3093805280 date "2021-01-01" @default.
- W3093805280 modified "2023-10-05" @default.
- W3093805280 title "Immunotoxic mechanisms of cigarette smoke and heat-not-burn tobacco vapor on Jurkat T cell functions" @default.
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- W3093805280 doi "https://doi.org/10.1016/j.envpol.2020.115863" @default.
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