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- W3093807067 abstract "SUMMARY Microtubule (MT)-associated proteins (MAPs) regulate intracellular transport by selectively recruiting or excluding kinesin and dynein motors from MTs. We used single-molecule and cryo-electron imaging to determine the mechanism of MAP-motor interactions in vitro. Unexpectedly, we found that the regulatory role of a MAP cannot be predicted based on whether it overlaps with the motor binding site or forms liquid condensates on the MT. Although the MT binding domain (MTBD) of MAP7 overlaps with the kinesin-1 binding site, tethering of kinesin-1 by the MAP7 projection domain supersedes this inhibition and results in biphasic regulation of kinesin-1 motility. Conversely, the MTBD of tau inhibits dynein motility without overlapping with the dynein binding site or by forming tau islands on the MT. Our results indicate that MAPs sort intracellular cargos moving in both directions, as neither dynein nor kinesin can walk on a MAP-coated MT without favorably interacting with that MAP. HIGHLIGHTS MAP7 binds to a novel site and can coexist with tau on the MT. Kinesin-1 motility is biphasically regulated by MAP7 accumulation on the microtubule. MT decoration of MAPs inhibits motors even when they do not block the motor binding site. Motors need to interact with a MAP to walk on MAP-decorated MTs" @default.
- W3093807067 created "2020-10-29" @default.
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- W3093807067 date "2020-10-23" @default.
- W3093807067 modified "2023-09-23" @default.
- W3093807067 title "The mechanism of motor inhibition by microtubule-associated proteins" @default.
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- W3093807067 cites W2013380493 @default.
- W3093807067 cites W2019578272 @default.
- W3093807067 cites W2021688560 @default.
- W3093807067 cites W2022214576 @default.
- W3093807067 cites W2022782971 @default.
- W3093807067 cites W2029667189 @default.
- W3093807067 cites W2034336561 @default.
- W3093807067 cites W2037415133 @default.
- W3093807067 cites W2039348923 @default.
- W3093807067 cites W2060872117 @default.
- W3093807067 cites W2068989347 @default.
- W3093807067 cites W2077699648 @default.
- W3093807067 cites W2085978470 @default.
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- W3093807067 cites W2126023465 @default.
- W3093807067 cites W2142161929 @default.
- W3093807067 cites W2150881786 @default.
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- W3093807067 cites W2298933030 @default.
- W3093807067 cites W2411563451 @default.
- W3093807067 cites W2479788400 @default.
- W3093807067 cites W2517060216 @default.
- W3093807067 cites W2551607099 @default.
- W3093807067 cites W2593511418 @default.
- W3093807067 cites W2607503492 @default.
- W3093807067 cites W2623370846 @default.
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- W3093807067 cites W2800724568 @default.
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- W3093807067 cites W2913590313 @default.
- W3093807067 cites W2913674846 @default.
- W3093807067 cites W2946253886 @default.
- W3093807067 cites W2949380999 @default.
- W3093807067 cites W2953010568 @default.
- W3093807067 cites W2967288961 @default.
- W3093807067 cites W2971448217 @default.
- W3093807067 cites W2971541306 @default.
- W3093807067 cites W2973026428 @default.
- W3093807067 cites W2980548480 @default.
- W3093807067 cites W2983128867 @default.
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- W3093807067 cites W2986798491 @default.
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- W3093807067 cites W3008749317 @default.
- W3093807067 cites W3009426597 @default.
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- W3093807067 doi "https://doi.org/10.1101/2020.10.22.351346" @default.
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