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• W3093886123 abstract "This study is concerned with the isolation of red complex pathogens, identifying them by a new molecular method as the first locally used and described procedure and characterizing these pathogens by their phenotypic features and biofilms. Gingival fluids were sampled from chronic periodontitis and inoculated into three types of culture media, Schaedler Anaerobe Agar, Tannerella forsythia (TF) agar, and Trypton Yeast extracts Gelatin Volatile fatty acids and Serum (TYGVS) agar. The different appearing colonies were purified and identified by Loop- Mediated Isothermal Amplification protocol (LAMP) for the detection of red complex species. Bacterial biofilm was estimated in term of mono- and polytypic biofilm by measuring the absorbance of a crystal violet- stained biofilm formed in a microtiter plate. Different forms of colonies appeared at the primary isolation. LAMP method was of a significant value for perfect rapid identification of the target species of extracted DNA or intact cells within half an hour. The three types of red complex pathogens were simultaneously detected in the same gingival fluid sample. They formed mono- and polymicrobial biofilms in a synergistic manner particularly the two intimates P. gingivalis and T. denticola. In conclusion, the updated LAMP molecular protocol was attractive method for the diagnosis of red complex pathogens which showed great morphological variations. P. gingivalis fortified the growth of the other two to establish polymicrobial biofilms in a synergistic manner." @default.
• W3093886123 created "2020-10-29" @default.
• W3093886123 creator A5029201939 @default.
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• W3093886123 date "2020-09-02" @default.
• W3093886123 modified "2023-10-18" @default.
• W3093886123 title "Identification of Red Complex Pathogens Group from Chronic Periodontitis Patients in Mosul City" @default.
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• W3093886123 doi "https://doi.org/10.33899/rjs.2020.166307" @default.
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