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- W3093929218 abstract "Species formerly classified within the genera Lactobacillus and Pediococcus have been studied extensively at the genomic level. To accommodate their exceptional functional diversity, the over 270 species were recently reclassified into 26 distinct genera. Despite their relevance to both academia and industry, methods that allow detailed exploration of their ecology are still limited by low resolution, high cost, or copy number variations. The approach described here makes use of a single-copy marker gene which outperforms other markers with regard to species-level resolution and availability of reference sequences (98% coverage). The tool was validated against a mock community and used to address diversity of lactobacilli and community structure in various environmental matrices. Such analyses can now be performed at a broader scale to assess and monitor the assembly, structure, and function of communities of lactobacilli at the species level (and, in some cases, even at the subspecies level) across a wide range of academic and commercial applications." @default.
- W3093929218 created "2020-10-29" @default.
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- W3093929218 date "2020-12-17" @default.
- W3093929218 modified "2023-10-16" @default.
- W3093929218 title "Phylotype-Level Characterization of Complex Communities of Lactobacilli Using a High-Throughput, High-Resolution Phenylalanyl-tRNA Synthetase ( <i>pheS</i> ) Gene Amplicon Sequencing Approach" @default.
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- W3093929218 doi "https://doi.org/10.1128/aem.02191-20" @default.
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