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- W3094039063 abstract "Abstract Ethnopharmacological relevance Rhamnella gilgitica Mansf. et Melch. (སང་ལང་།, RG) is a traditional Tibetan medicinal plant that is currently grown throughout Tibet. According to the theory of Tibetan medicine, RG is efficient for removing rheumatism, reducing swelling, and relieving pain. Hence, it has been used for the treatment of rheumatoid arthritis (RA) in Tibet for many years. However, there are no previous reports on the anti-RA activities of ethyl acetate extract of RG (RGEA). Aim of the study This study aimed to explore the anti-RA effect and mechanism of RGEA on collagen-induced arthritis (CIA) in rats. Materials and methods The CIA model was established in male Wister rats by intradermal injection of bovine type II collagen and Complete Freund’s Adjuvant at the base of the tail and left sole, respectively. The rats were orally administered with RGEA (9.71, 19.43, or 38.85 mg/kg) for 23 days. The body weight, swelling volume, arthritis index score, thymus and spleen indices, and pathological changes were observed to evaluate the effect of RGEA on RA. Furthermore, the inflammatory cytokines in serum, such as interleukin1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin6 (IL-6), interleukin17 (IL-17), interferon-γ (INF-γ), interleukin4 (IL-4), and interleukin10 (IL-10) were measured by enzyme linked immunosorbent assay (ELISA) to explore the anti-inflammatory effects of RGEA. The terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining was used to examine apoptosis. Finally, the protein and gene expression of B-cell lymphoma-2-associated X (Bax), B-cell lymphoma 2 (Bcl-2), Caspase3, janus-activated kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), suppressor of cytokine signaling1 (SOCS1), and 3 (SOCS3) in synovial tissue were detected using immunohistochemistry and real-time quantitative polymerase chain reaction (RT-qPCR). Results After the treatment with RGEA, the body weight of rats was restored, both the arthritis index and paw swelling were suppressed, and spleen and thymus indices were decreased. RGEA reduced the inflammatory cells and synovial hyperplasia in the synovial tissue of the knee joint, and suppressed bone erosion. Meanwhile, RGEA decreased the levels of IL-1β, IL-6, IL-17, TNF-α, and INF-γ, while increased the levels of IL-4 and IL-10. TUNEL fluorescence apoptosis results confirmed that RGEA obviously promoted the apoptosis of synovial cells. Further studies showed that RGEA inhibited the proteins and mRNAs expression of JAK2 and STAT3 as well as increased the proteins and mRNAs expression of SOCS1 and SOCS3. In addition, RGEA upregulated the expression of Bax and Caspase3, and downregulated the expression of Bcl-2. Conclusion The anti-RA effectof RGEA might be related to the promotion of apoptosis and inhibition of inflammation, which regulated the JAK-STAT pathway." @default.
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- W3094039063 date "2021-03-01" @default.
- W3094039063 modified "2023-09-23" @default.
- W3094039063 title "Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway" @default.
- W3094039063 cites W1510351486 @default.
- W3094039063 cites W1878991695 @default.
- W3094039063 cites W1964275122 @default.
- W3094039063 cites W1978180376 @default.
- W3094039063 cites W1992465548 @default.
- W3094039063 cites W2001578876 @default.
- W3094039063 cites W2011317507 @default.
- W3094039063 cites W2016540525 @default.
- W3094039063 cites W2018077387 @default.
- W3094039063 cites W2019015062 @default.
- W3094039063 cites W2037382204 @default.
- W3094039063 cites W2065644511 @default.
- W3094039063 cites W2066960433 @default.
- W3094039063 cites W2077574194 @default.
- W3094039063 cites W2080817427 @default.
- W3094039063 cites W2093277474 @default.
- W3094039063 cites W2094275117 @default.
- W3094039063 cites W2106171259 @default.
- W3094039063 cites W2109134094 @default.
- W3094039063 cites W2109955020 @default.
- W3094039063 cites W2110826367 @default.
- W3094039063 cites W2122083689 @default.
- W3094039063 cites W2133692536 @default.
- W3094039063 cites W2134714663 @default.
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- W3094039063 cites W2171784256 @default.
- W3094039063 cites W2406597180 @default.
- W3094039063 cites W2415150972 @default.
- W3094039063 cites W2468398591 @default.
- W3094039063 cites W2546339992 @default.
- W3094039063 cites W2562776611 @default.
- W3094039063 cites W2589060305 @default.
- W3094039063 cites W2591299403 @default.
- W3094039063 cites W2593351382 @default.
- W3094039063 cites W2608454940 @default.
- W3094039063 cites W2615203220 @default.
- W3094039063 cites W2623617647 @default.
- W3094039063 cites W2750825959 @default.
- W3094039063 cites W2777315115 @default.
- W3094039063 cites W2793658759 @default.
- W3094039063 cites W2805170482 @default.
- W3094039063 cites W2896491161 @default.
- W3094039063 cites W2904650866 @default.
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- W3094039063 doi "https://doi.org/10.1016/j.jep.2020.113514" @default.
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