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- W3094152034 abstract "Horse milk exosomes have been isolated and purified via the developed technology, and their microscopic and biochemical analyzes have been carried out. It was shown that the gel-filtration on the Ultrogel A4 resin can significantly reduce the amount of milk proteins co-isolated with exosomes. Methods for the isolation of nucleic acids from the preparations at various purification stages were proposed, and the content of nucleic acids in horse milk exosomes was analyzed. It was demonstrated that horse milk exosome preparations are not toxic to human cell cultures. The prospects of using horse milk exosomes for drug delivery into cell cultures are discussed. exosomes, horse milk, exosome isolation, nucleic acids The research carried out by S.E. Sedykh, A.E. Kuleshova and E.E. Burkova was financially supported by the Russian Scientific Foundation (project no. 18-74-10055 to S.E. Sedykh); the research by G.A. Nevinskii (MALDI TOF MS/MS analysis) was supported by the basic budget financing project no. ICBFM SB RAS # АААА-А17-117020210023-1 (to G.A. Nevinsky)." @default.
- W3094152034 created "2020-10-29" @default.
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- W3094152034 date "2020-01-01" @default.
- W3094152034 modified "2023-09-26" @default.
- W3094152034 title "Horse Milk Exosomes: Isolation, Microscopic and Biochemical Analysis, and Prospects of Use" @default.
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- W3094152034 doi "https://doi.org/10.21519/0234-2758-2020-36-5-62-71" @default.
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