Matches in SemOpenAlex for { <https://semopenalex.org/work/W3094391173> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3094391173 abstract "Abstract Background: ChAdOx1 nCoV-19 is a recombinant adenovirus vaccine candidate against SARS-CoV-2. Although replication defective in normal cells, 28kbp of adenovirus genes are delivered to the cell nucleus alongside the SARS-CoV-2 S glycoprotein gene. Methods: We used direct RNA sequencing to analyse transcript expression from the ChAdOx1 nCoV-19 genome in human MRC-5 and A549 cell lines that are non-permissive for vector replication alongside the replication permissive cell line, HEK293. In addition, we used quantitative proteomics to study over time the proteome and phosphoproteome of A549 and MRC5 cells infected with the ChAdOx1 nCoV-19 vaccine candidate. Results: The expected SARS-CoV-2 S coding transcript dominated in all cell lines. We also detected rare S transcripts with aberrant splice patterns or polyadenylation site usage. Adenovirus vector transcripts were almost absent in MRC-5 cells but in A549 cells there was a broader repertoire of adenoviral gene expression at very low levels. Proteomically, in addition to S glycoprotein, we detected multiple adenovirus proteins in A549 cells compared to just one in MRC5 cells. Conclusions: Overall the ChAdOx1 nCoV-19 vaccine’s transcriptomic and proteomic repertoire is as expected. The combined transcriptomic and proteomics approaches provide an unparalleled insight into the behaviour of this important class of vaccine candidate and illustrate the potential of this technique to inform future viral vaccine vector design." @default.
- W3094391173 created "2020-10-29" @default.
- W3094391173 creator A5026792826 @default.
- W3094391173 creator A5048188220 @default.
- W3094391173 creator A5056810919 @default.
- W3094391173 creator A5061020486 @default.
- W3094391173 creator A5076940927 @default.
- W3094391173 creator A5078208432 @default.
- W3094391173 creator A5086749128 @default.
- W3094391173 creator A5089670625 @default.
- W3094391173 date "2020-10-20" @default.
- W3094391173 modified "2023-09-23" @default.
- W3094391173 title "SARS-CoV-2 candidate vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals a normal low range of viral backbone gene expression alongside very high levels of SARS-CoV-2 S glycoprotein expression." @default.
- W3094391173 doi "https://doi.org/10.21203/rs.3.rs-94837/v1" @default.
- W3094391173 hasPublicationYear "2020" @default.
- W3094391173 type Work @default.
- W3094391173 sameAs 3094391173 @default.
- W3094391173 citedByCount "2" @default.
- W3094391173 countsByYear W30943911732021 @default.
- W3094391173 countsByYear W30943911732023 @default.
- W3094391173 crossrefType "posted-content" @default.
- W3094391173 hasAuthorship W3094391173A5026792826 @default.
- W3094391173 hasAuthorship W3094391173A5048188220 @default.
- W3094391173 hasAuthorship W3094391173A5056810919 @default.
- W3094391173 hasAuthorship W3094391173A5061020486 @default.
- W3094391173 hasAuthorship W3094391173A5076940927 @default.
- W3094391173 hasAuthorship W3094391173A5078208432 @default.
- W3094391173 hasAuthorship W3094391173A5086749128 @default.
- W3094391173 hasAuthorship W3094391173A5089670625 @default.
- W3094391173 hasBestOaLocation W30943911731 @default.
- W3094391173 hasConcept C104317684 @default.
- W3094391173 hasConcept C104397665 @default.
- W3094391173 hasConcept C140704245 @default.
- W3094391173 hasConcept C150194340 @default.
- W3094391173 hasConcept C159047783 @default.
- W3094391173 hasConcept C162317418 @default.
- W3094391173 hasConcept C2522874641 @default.
- W3094391173 hasConcept C32470452 @default.
- W3094391173 hasConcept C40767141 @default.
- W3094391173 hasConcept C54355233 @default.
- W3094391173 hasConcept C81885089 @default.
- W3094391173 hasConcept C86803240 @default.
- W3094391173 hasConceptScore W3094391173C104317684 @default.
- W3094391173 hasConceptScore W3094391173C104397665 @default.
- W3094391173 hasConceptScore W3094391173C140704245 @default.
- W3094391173 hasConceptScore W3094391173C150194340 @default.
- W3094391173 hasConceptScore W3094391173C159047783 @default.
- W3094391173 hasConceptScore W3094391173C162317418 @default.
- W3094391173 hasConceptScore W3094391173C2522874641 @default.
- W3094391173 hasConceptScore W3094391173C32470452 @default.
- W3094391173 hasConceptScore W3094391173C40767141 @default.
- W3094391173 hasConceptScore W3094391173C54355233 @default.
- W3094391173 hasConceptScore W3094391173C81885089 @default.
- W3094391173 hasConceptScore W3094391173C86803240 @default.
- W3094391173 hasLocation W30943911731 @default.
- W3094391173 hasLocation W30943911732 @default.
- W3094391173 hasOpenAccess W3094391173 @default.
- W3094391173 hasPrimaryLocation W30943911731 @default.
- W3094391173 hasRelatedWork W1608862421 @default.
- W3094391173 hasRelatedWork W1825162875 @default.
- W3094391173 hasRelatedWork W1990141933 @default.
- W3094391173 hasRelatedWork W1991126365 @default.
- W3094391173 hasRelatedWork W2009966535 @default.
- W3094391173 hasRelatedWork W2010478346 @default.
- W3094391173 hasRelatedWork W2021255975 @default.
- W3094391173 hasRelatedWork W2102579905 @default.
- W3094391173 hasRelatedWork W2406270448 @default.
- W3094391173 hasRelatedWork W3165029026 @default.
- W3094391173 isParatext "false" @default.
- W3094391173 isRetracted "false" @default.
- W3094391173 magId "3094391173" @default.
- W3094391173 workType "article" @default.