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• W3094508306 abstract "The use of CRISPR-Cas and RNA-guided endonucleases has drastically changed research strategies for understanding and exploiting gene function, particularly for the generation of gene-edited animal models. This has resulted in an explosion in the number of gene-edited species, including highly biomedically relevant pig models. However, even with error-free DNA insertion or deletion, edited genes are occasionally not expressed and/or translated as expected. Therefore, there is a need to validate the expression outcomes gene modifications in vitro before investing in the costly generation of a gene-edited animal. Unfortunately, many gene targets are tissue specific and/or not expressed in cultured primary cells, making validation difficult without generating an animal. In this study, using pigs as a proof of concept, we show that CRISPR-dCas9 transcriptional activators can be used to validate functional transgene insertion in nonexpressing easily cultured cells such as fibroblasts. This is a tool that can be used across disciplines and animal species to save time and resources by verifying expected outcomes of gene edits before generating live animals." @default.
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• W3094508306 date "2020-10-01" @default.
• W3094508306 modified "2023-09-26" @default.
• W3094508306 title "<i>In Vitro</i> Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators" @default.
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• W3094508306 doi "https://doi.org/10.1089/crispr.2020.0037" @default.
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