FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3094563670> ?p ?o ?g. }

• W3094563670 endingPage "1" @default.
• W3094563670 startingPage "1" @default.
• W3094563670 abstract "Abnormal cell proliferation and invasion of vascular smooth muscle cells are among the primary causes of cardiovascular disease. Studies have shown that microRNA(miR)‑342‑5p participates in the development of cardiovascular diseases. The current study aimed to explore the role of miR‑342‑5p in the proliferation and differentiation of mouse aortic vascular smooth muscle (MOVAS) cells. MOVAS cells were transfected with miR‑342‑5p mimics, miR‑342‑5p inhibitor or their respective negative controls, and co‑transfected with small interfering (si)RNA targeting phosphatidylinositol 3‑kinase regulatory subunit α (PIK3R1) and miR‑342‑5p inhibitor. The cell proliferation of MOVAS cells was detected using the Cell Counting Kit‑8, while cell migration and cell invasion were investigated using a wound healing and Transwell assays, respectively. Target genes for miR‑342‑5p were confirmed using reverse transcription‑quantitative PCR (RT‑qPCR) and dual luciferase reporter assay. The relative mRNA and protein expression levels of miR‑342‑5p were measured using RT‑qPCR and western blot analysis. MOVAS cells were treated with a PI3K inhibitor (LY294002) to explore the role of miR‑342‑5p on the Akt pathway. The results revealed that miR‑342‑5p mimics promoted cell viability, migration and invasion, and increased the expression of vimentin and phosphorylated‑Akt but reduced a‑smooth muscle actin (α‑SMA) and PIK3R1 expression. However, miR‑342‑5p inhibitor produced the opposite effects. PIK3R1 was the target gene for miR‑342‑5p and the effect of siPIK3R1 on MOVAS cells was similar to that of miR‑342‑5p mimics, while siPIK3R1 partially reversed the effect of miR‑342‑5p inhibitor on MOVAS cells. The Akt signaling pathway was activated by miR‑342‑5p mimics or siPIK3R1. Moreover, miR‑342‑5p mimics partially activated the Akt signaling pathway inhibited by LY294002. MiR‑342‑5p could promote the proliferation and differentiation of MOVAS and phenotypic transformation. The mechanism behind these processes may be associated with the activation of the Akt signaling pathway induced by PIK3R1 inhibition." @default.
• W3094563670 created "2020-10-29" @default.
• W3094563670 creator A5014273615 @default.
• W3094563670 creator A5031277374 @default.
• W3094563670 creator A5051398666 @default.
• W3094563670 creator A5080632365 @default.
• W3094563670 creator A5081841587 @default.
• W3094563670 date "2020-10-22" @default.
• W3094563670 modified "2023-09-23" @default.
• W3094563670 title "MicroRNA‑342‑5p activates the Akt signaling pathway by downregulating PIK3R1 to modify the proliferation and differentiation of vascular smooth muscle cells" @default.
• W3094563670 cites W1488925967 @default.
• W3094563670 cites W1505332570 @default.
• W3094563670 cites W1583375002 @default.
• W3094563670 cites W1963782714 @default.
• W3094563670 cites W1976649309 @default.
• W3094563670 cites W1983508037 @default.
• W3094563670 cites W1985807131 @default.
• W3094563670 cites W1987991415 @default.
• W3094563670 cites W1991536401 @default.
• W3094563670 cites W2034973443 @default.
• W3094563670 cites W2044290370 @default.
• W3094563670 cites W2051209145 @default.
• W3094563670 cites W2056639499 @default.
• W3094563670 cites W2057643476 @default.
• W3094563670 cites W2062689336 @default.
• W3094563670 cites W2072038660 @default.
• W3094563670 cites W2097607398 @default.
• W3094563670 cites W2107277218 @default.
• W3094563670 cites W2129228205 @default.
• W3094563670 cites W2170344892 @default.
• W3094563670 cites W2192080449 @default.
• W3094563670 cites W2267105610 @default.
• W3094563670 cites W2291810277 @default.
• W3094563670 cites W2316979953 @default.
• W3094563670 cites W2346343701 @default.
• W3094563670 cites W2350153048 @default.
• W3094563670 cites W2423767910 @default.
• W3094563670 cites W2526541915 @default.
• W3094563670 cites W2546803836 @default.
• W3094563670 cites W2548162453 @default.
• W3094563670 cites W2591218850 @default.
• W3094563670 cites W2597137467 @default.
• W3094563670 cites W2615835286 @default.
• W3094563670 cites W2740104770 @default.
• W3094563670 cites W2799478334 @default.
• W3094563670 cites W2799491207 @default.
• W3094563670 cites W2799805941 @default.
• W3094563670 cites W2803344251 @default.
• W3094563670 cites W2805684555 @default.
• W3094563670 cites W2921097213 @default.
• W3094563670 cites W2950485637 @default.
• W3094563670 cites W2986716992 @default.
• W3094563670 cites W3013587187 @default.
• W3094563670 doi "https://doi.org/10.3892/etm.2020.9369" @default.
• W3094563670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7646700" @default.
• W3094563670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33193844" @default.
• W3094563670 hasPublicationYear "2020" @default.
• W3094563670 type Work @default.
• W3094563670 sameAs 3094563670 @default.
• W3094563670 citedByCount "4" @default.
• W3094563670 countsByYear W30945636702021 @default.
• W3094563670 countsByYear W30945636702022 @default.
• W3094563670 countsByYear W30945636702023 @default.
• W3094563670 crossrefType "journal-article" @default.
• W3094563670 hasAuthorship W3094563670A5014273615 @default.
• W3094563670 hasAuthorship W3094563670A5031277374 @default.
• W3094563670 hasAuthorship W3094563670A5051398666 @default.
• W3094563670 hasAuthorship W3094563670A5080632365 @default.
• W3094563670 hasAuthorship W3094563670A5081841587 @default.
• W3094563670 hasBestOaLocation W30945636701 @default.
• W3094563670 hasConcept C104317684 @default.
• W3094563670 hasConcept C134018914 @default.
• W3094563670 hasConcept C137738243 @default.
• W3094563670 hasConcept C145059251 @default.
• W3094563670 hasConcept C1491633281 @default.
• W3094563670 hasConcept C153911025 @default.
• W3094563670 hasConcept C22615655 @default.
• W3094563670 hasConcept C2776912716 @default.
• W3094563670 hasConcept C2779395532 @default.
• W3094563670 hasConcept C29537977 @default.
• W3094563670 hasConcept C2992686903 @default.
• W3094563670 hasConcept C502942594 @default.
• W3094563670 hasConcept C53227056 @default.
• W3094563670 hasConcept C54009773 @default.
• W3094563670 hasConcept C54355233 @default.
• W3094563670 hasConcept C55493867 @default.
• W3094563670 hasConcept C62112901 @default.
• W3094563670 hasConcept C62478195 @default.
• W3094563670 hasConcept C75217442 @default.
• W3094563670 hasConcept C81885089 @default.
• W3094563670 hasConcept C86554907 @default.
• W3094563670 hasConcept C86803240 @default.
• W3094563670 hasConcept C95444343 @default.
• W3094563670 hasConceptScore W3094563670C104317684 @default.
• W3094563670 hasConceptScore W3094563670C134018914 @default.
• W3094563670 hasConceptScore W3094563670C137738243 @default.
• W3094563670 hasConceptScore W3094563670C145059251 @default.
• W3094563670 hasConceptScore W3094563670C1491633281 @default.

• next