Matches in SemOpenAlex for { <https://semopenalex.org/work/W3094889035> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W3094889035 endingPage "e0241647" @default.
- W3094889035 startingPage "e0241647" @default.
- W3094889035 abstract "Mycoplasma synoviae infection occurs worldwide, leading to considerable economic losses in the chicken and turkey industry due to infectious synovitis, respiratory diseases and eggshell apex abnormalities. Control programs against M. synoviae infection are based on eradication, vaccination and medication with antimicrobial agents. Prudent use of antibiotics can be improved greatly by the determination of antibiotic susceptibility prior to the treatment. However, the conventional broth or agar microdilution is very labor-intensive and time-consuming method. Thus, there is an increasing need for rapid antimicrobial susceptibility tests in order to guide antibiotic therapy more effectively. The aim of this study was to develop mismatch amplification mutation assays (MAMAs) to detect resistance-associated mutations in M. synoviae. M. synoviae strains with previously determined minimal inhibitory concentrations (MICs) and whole genomes (n = 92) were used for target selection and assay specification. For the evaluation of the developed assays, 20 clinical samples and an additional 20 M. synoviae isolates derived from these specimens were also included in this study. MIC values of these 20 isolates were determined by broth microdilution method. Five MAMAs were designed to identify elevated MICs of fluoroquinolones, while three MAMAs were developed to detect decreased susceptibility to macrolides and lincomycin. The sensitivity of the MAMA tests varied between 102−104 template copy number/reaction depending on the assay. Clinical samples showed identical genotype calls with the M. synoviae isolates derived from the corresponding specimens in each case. Supporting the results of conventional in vitro sensitivity tests, our approach provides a feasible tool for diagnostics. Rapidity, robustness and cost-effectiveness are powerful advantages of the developed assays. Supporting prudent antibiotic usage instead of empirical treatment, the use of this method can reduce significantly the economic impact of M. synoviae in the poultry industry and decrease bacterial resistance-related public health concerns." @default.
- W3094889035 created "2020-11-09" @default.
- W3094889035 creator A5025093794 @default.
- W3094889035 creator A5050941901 @default.
- W3094889035 creator A5061343756 @default.
- W3094889035 creator A5064636951 @default.
- W3094889035 creator A5073944050 @default.
- W3094889035 creator A5081213334 @default.
- W3094889035 creator A5085698529 @default.
- W3094889035 date "2020-10-30" @default.
- W3094889035 modified "2023-10-06" @default.
- W3094889035 title "Development of molecular assays for the rapid and cost-effective determination of fluoroquinolone, macrolide and lincosamide susceptibility of Mycoplasma synoviae isolates" @default.
- W3094889035 cites W1951848410 @default.
- W3094889035 cites W1963768313 @default.
- W3094889035 cites W1969307309 @default.
- W3094889035 cites W1976672951 @default.
- W3094889035 cites W1982566730 @default.
- W3094889035 cites W2006092569 @default.
- W3094889035 cites W2013073820 @default.
- W3094889035 cites W2082897377 @default.
- W3094889035 cites W2128880918 @default.
- W3094889035 cites W2135110560 @default.
- W3094889035 cites W2496188222 @default.
- W3094889035 cites W2514261101 @default.
- W3094889035 cites W2768310190 @default.
- W3094889035 cites W2770547684 @default.
- W3094889035 cites W2989157446 @default.
- W3094889035 cites W3022678454 @default.
- W3094889035 doi "https://doi.org/10.1371/journal.pone.0241647" @default.
- W3094889035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7598480" @default.
- W3094889035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33125410" @default.
- W3094889035 hasPublicationYear "2020" @default.
- W3094889035 type Work @default.
- W3094889035 sameAs 3094889035 @default.
- W3094889035 citedByCount "2" @default.
- W3094889035 countsByYear W30948890352021 @default.
- W3094889035 crossrefType "journal-article" @default.
- W3094889035 hasAuthorship W3094889035A5025093794 @default.
- W3094889035 hasAuthorship W3094889035A5050941901 @default.
- W3094889035 hasAuthorship W3094889035A5061343756 @default.
- W3094889035 hasAuthorship W3094889035A5064636951 @default.
- W3094889035 hasAuthorship W3094889035A5073944050 @default.
- W3094889035 hasAuthorship W3094889035A5081213334 @default.
- W3094889035 hasAuthorship W3094889035A5085698529 @default.
- W3094889035 hasBestOaLocation W30948890351 @default.
- W3094889035 hasConcept C159047783 @default.
- W3094889035 hasConcept C176947019 @default.
- W3094889035 hasConcept C2776100946 @default.
- W3094889035 hasConcept C2778838687 @default.
- W3094889035 hasConcept C2780374374 @default.
- W3094889035 hasConcept C2780950330 @default.
- W3094889035 hasConcept C2781151156 @default.
- W3094889035 hasConcept C2910317154 @default.
- W3094889035 hasConcept C501593827 @default.
- W3094889035 hasConcept C523546767 @default.
- W3094889035 hasConcept C54355233 @default.
- W3094889035 hasConcept C86803240 @default.
- W3094889035 hasConcept C89423630 @default.
- W3094889035 hasConceptScore W3094889035C159047783 @default.
- W3094889035 hasConceptScore W3094889035C176947019 @default.
- W3094889035 hasConceptScore W3094889035C2776100946 @default.
- W3094889035 hasConceptScore W3094889035C2778838687 @default.
- W3094889035 hasConceptScore W3094889035C2780374374 @default.
- W3094889035 hasConceptScore W3094889035C2780950330 @default.
- W3094889035 hasConceptScore W3094889035C2781151156 @default.
- W3094889035 hasConceptScore W3094889035C2910317154 @default.
- W3094889035 hasConceptScore W3094889035C501593827 @default.
- W3094889035 hasConceptScore W3094889035C523546767 @default.
- W3094889035 hasConceptScore W3094889035C54355233 @default.
- W3094889035 hasConceptScore W3094889035C86803240 @default.
- W3094889035 hasConceptScore W3094889035C89423630 @default.
- W3094889035 hasFunder F4320322192 @default.
- W3094889035 hasFunder F4320326762 @default.
- W3094889035 hasIssue "10" @default.
- W3094889035 hasLocation W30948890351 @default.
- W3094889035 hasLocation W30948890352 @default.
- W3094889035 hasLocation W30948890353 @default.
- W3094889035 hasOpenAccess W3094889035 @default.
- W3094889035 hasPrimaryLocation W30948890351 @default.
- W3094889035 hasRelatedWork W2090755692 @default.
- W3094889035 hasRelatedWork W2096363361 @default.
- W3094889035 hasRelatedWork W2114260822 @default.
- W3094889035 hasRelatedWork W2151828834 @default.
- W3094889035 hasRelatedWork W2333869474 @default.
- W3094889035 hasRelatedWork W2403412381 @default.
- W3094889035 hasRelatedWork W2512784365 @default.
- W3094889035 hasRelatedWork W2978065364 @default.
- W3094889035 hasRelatedWork W3094889035 @default.
- W3094889035 hasRelatedWork W3111602953 @default.
- W3094889035 hasVolume "15" @default.
- W3094889035 isParatext "false" @default.
- W3094889035 isRetracted "false" @default.
- W3094889035 magId "3094889035" @default.
- W3094889035 workType "article" @default.