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• W3095642371 endingPage "129158" @default.
• W3095642371 startingPage "129158" @default.
• W3095642371 abstract "By constructing antibody-antigen-aptamer sandwich structure, an electrochemical biosensor for the determination of sulfamethazine was fabricated using AuOct-PEI-C 3 N 4 composites and aptamer-Au@Pt-MB signal labels. • An electrochemical biosensor with an antibody-antigen-aptamer sandwich structure was proposed for SMZ determination. • AuOct-PEI-C 3 N 4 composites with good conductivity and great specific surface area were modified on AuE to immobilize Ab. • Au@Pt core-shell nanocomposites loaded with redox probe MB and aptamer were used as signal labels. • The electrochemical biosensor exbibited good selectivity and reproducibility. Sulfamethazine (SMZ) is one of the most widely used sulfonamides and is frequently detected in animal-derived food bringing a threat to human health. Accordingly, establishing a rapid, convenient, and sensitive analytical method for SMZ on-site detection is of significance to improve food safety. In this work, a sandwich-type electrochemical biosensor is described for the determination of sulfamethazine, based on the high affinity of antibody and aptamer to analytes. Subsequently, sulfamethazine monoclonal antibody (Ab) was immobilized on the gold electrode modified with octahedral gold nanoparticles (AuOct) and the poly (ethylenimine) (PEI) functionalized graphitic carbon nitride (g-C 3 N 4 ) nanosheets. The AuOct-PEI-C 3 N 4 composites were used as a sensing platform for the immobilization of Ab through Au-N bond. Wherein, Au@Pt core-shell nanocomposites by loading methylene blue (MB) and aptamers were used as signal labels. Once SMZ existed, the Ab/SMZ/G-quadruplex complex is formed and the redox probe MB at Au@Pt core-shell nanoparticles is linked on the electrode. The current changes in square wave voltammetry (SWV) are linearly related to the logarithm of SMZ concentrations ranged from 0.0001–100 ng mL −1 . And the relevant detection limit of 0.069 pg mL −1 was obtained. The biosensor also exhibits good selectivity and reproducibility, providing a good platform for SMZ determination." @default.
• W3095642371 created "2020-11-09" @default.
• W3095642371 creator A5008884322 @default.
• W3095642371 creator A5041584117 @default.
• W3095642371 date "2021-02-01" @default.
• W3095642371 modified "2023-10-13" @default.
• W3095642371 title "Ultrasensitive sandwich-type electrochemical biosensor based on octahedral gold nanoparticles modified poly (ethylenimine) functionalized graphitic carbon nitride nanosheets for the determination of sulfamethazine" @default.
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• W3095642371 doi "https://doi.org/10.1016/j.snb.2020.129158" @default.
• W3095642371 hasPublicationYear "2021" @default.
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