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• W3096002958 abstract "8-Azaguanine (1) is a special 1,2,3-triazole containing natural product that possesses potent antibacterial and antitumor activities. In the present study, the entire 8-azaguanine biosynthetic gene cluster was located from Streptomyces CGMCC4.1633. Targeted gene disruption, heterologous expression analysis, and feeding experiments identified crucial genes for 8-azaguanine production. Moreover, we characterized the structure of two novel metabolites, analyzed NO (or reactive nitrogen species) related genes 8-azgA/B and radical SAM enzyme homologous 8-AzgG, and verified the non-enzymatic ring formation reaction of 8-azaguanine 1,2,3-triazole. All of the data and presumptions provide insight into the timing and mechanism of the enzymatic and non-enzymatic pathway that produce 8-azaguanine-type 1,2,3-triazole." @default.
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• W3096002958 date "2020-11-05" @default.
• W3096002958 modified "2023-09-27" @default.
• W3096002958 title "Identification of 8-Azaguanine Biosynthesis–Related Genes Provides Insight Into the Enzymatic and Non-enzymatic Biosynthetic Pathway for 1,2,3-Triazole" @default.
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• W3096002958 doi "https://doi.org/10.3389/fbioe.2020.603514" @default.
• W3096002958 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7674941" @default.
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