FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096003726> ?p ?o ?g. }

• W3096003726 endingPage "103479" @default.
• W3096003726 startingPage "103479" @default.
• W3096003726 abstract "Aspergillus fumigatus is a saprophytic fungal pathogen that is the cause of more than 300,000 life-threatening infections annually. Our understanding of pathogenesis and factors contributing to disease progression are limited. Development of rapid and versatile gene editing methodologies for A. fumigatus is essential. CRISPR-Cas9 mediated transformation has been widely used as a novel genome editing tool and has been used for a variety of editing techniques, such as protein tagging, gene deletions and site-directed mutagenesis in A. fumigatus. However, successful genome editing relies on time consuming, multi-step cloning procedures paired with the use of selection markers, which can result in a metabolic burden for the host and/or unintended transcriptional modifications at the site of integration. We have used an in vitro CRISPR-Cas9 assembly methodology to perform selection-free genome editing, including epitope tagging of proteins and site-directed mutagenesis. The repair template used during this transformation use 50 bp micro-homology arms and can be generated with a single PCR reaction or by purchasing synthesised single stranded oligonucleotides, decreasing the time required for complex construct synthesis." @default.
• W3096003726 created "2020-11-09" @default.
• W3096003726 creator A5022255783 @default.
• W3096003726 creator A5038725561 @default.
• W3096003726 creator A5046177603 @default.
• W3096003726 creator A5065643161 @default.
• W3096003726 creator A5073999198 @default.
• W3096003726 creator A5080818589 @default.
• W3096003726 date "2020-12-01" @default.
• W3096003726 modified "2023-10-16" @default.
• W3096003726 title "Development of a marker-free mutagenesis system using CRISPR-Cas9 in the pathogenic mould Aspergillus fumigatus" @default.
• W3096003726 cites W17439892 @default.
• W3096003726 cites W1888035769 @default.
• W3096003726 cites W1900589173 @default.
• W3096003726 cites W1965657692 @default.
• W3096003726 cites W1973084729 @default.
• W3096003726 cites W1979637843 @default.
• W3096003726 cites W2021133599 @default.
• W3096003726 cites W2031933178 @default.
• W3096003726 cites W2053403241 @default.
• W3096003726 cites W2057948698 @default.
• W3096003726 cites W2061057323 @default.
• W3096003726 cites W2066697723 @default.
• W3096003726 cites W2096209592 @default.
• W3096003726 cites W2115420193 @default.
• W3096003726 cites W2116534141 @default.
• W3096003726 cites W2118505677 @default.
• W3096003726 cites W2129495031 @default.
• W3096003726 cites W2154425766 @default.
• W3096003726 cites W2169244146 @default.
• W3096003726 cites W2174006038 @default.
• W3096003726 cites W2220028518 @default.
• W3096003726 cites W2325603122 @default.
• W3096003726 cites W2504680581 @default.
• W3096003726 cites W2529558525 @default.
• W3096003726 cites W2584885207 @default.
• W3096003726 cites W2611446801 @default.
• W3096003726 cites W2626248694 @default.
• W3096003726 cites W2767031614 @default.
• W3096003726 cites W2770016143 @default.
• W3096003726 cites W2783508188 @default.
• W3096003726 cites W2792675259 @default.
• W3096003726 cites W2805578580 @default.
• W3096003726 cites W2806820493 @default.
• W3096003726 cites W2883697525 @default.
• W3096003726 cites W2929562905 @default.
• W3096003726 cites W2951413534 @default.
• W3096003726 cites W2961923073 @default.
• W3096003726 cites W3002988646 @default.
• W3096003726 cites W3087503803 @default.
• W3096003726 cites W333206344 @default.
• W3096003726 cites W927857605 @default.
• W3096003726 doi "https://doi.org/10.1016/j.fgb.2020.103479" @default.
• W3096003726 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7768092" @default.
• W3096003726 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33122116" @default.
• W3096003726 hasPublicationYear "2020" @default.
• W3096003726 type Work @default.
• W3096003726 sameAs 3096003726 @default.
• W3096003726 citedByCount "25" @default.
• W3096003726 countsByYear W30960037262021 @default.
• W3096003726 countsByYear W30960037262022 @default.
• W3096003726 countsByYear W30960037262023 @default.
• W3096003726 crossrefType "journal-article" @default.
• W3096003726 hasAuthorship W3096003726A5022255783 @default.
• W3096003726 hasAuthorship W3096003726A5038725561 @default.
• W3096003726 hasAuthorship W3096003726A5046177603 @default.
• W3096003726 hasAuthorship W3096003726A5065643161 @default.
• W3096003726 hasAuthorship W3096003726A5073999198 @default.
• W3096003726 hasAuthorship W3096003726A5080818589 @default.
• W3096003726 hasBestOaLocation W30960037261 @default.
• W3096003726 hasConcept C104317684 @default.
• W3096003726 hasConcept C132455925 @default.
• W3096003726 hasConcept C141231307 @default.
• W3096003726 hasConcept C144501496 @default.
• W3096003726 hasConcept C16318435 @default.
• W3096003726 hasConcept C2779787849 @default.
• W3096003726 hasConcept C501734568 @default.
• W3096003726 hasConcept C54355233 @default.
• W3096003726 hasConcept C70721500 @default.
• W3096003726 hasConcept C86803240 @default.
• W3096003726 hasConcept C89423630 @default.
• W3096003726 hasConcept C98108389 @default.
• W3096003726 hasConceptScore W3096003726C104317684 @default.
• W3096003726 hasConceptScore W3096003726C132455925 @default.
• W3096003726 hasConceptScore W3096003726C141231307 @default.
• W3096003726 hasConceptScore W3096003726C144501496 @default.
• W3096003726 hasConceptScore W3096003726C16318435 @default.
• W3096003726 hasConceptScore W3096003726C2779787849 @default.
• W3096003726 hasConceptScore W3096003726C501734568 @default.
• W3096003726 hasConceptScore W3096003726C54355233 @default.
• W3096003726 hasConceptScore W3096003726C70721500 @default.
• W3096003726 hasConceptScore W3096003726C86803240 @default.
• W3096003726 hasConceptScore W3096003726C89423630 @default.
• W3096003726 hasConceptScore W3096003726C98108389 @default.
• W3096003726 hasFunder F4320311904 @default.
• W3096003726 hasFunder F4320334629 @default.
• W3096003726 hasLocation W30960037261 @default.
• W3096003726 hasLocation W30960037262 @default.

• next