Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096103422> ?p ?o ?g. }
- W3096103422 endingPage "109300" @default.
- W3096103422 startingPage "109300" @default.
- W3096103422 abstract "Suppression and modulation of the host immune response to parasitic nematodes have been extensively studied. In the present study, we cloned and produced recombinant electron transfer flavoprotein α subunit (ETFα) protein from Haemonchus contortus (rHCETFα), a parasitic nematode of small ruminants, and studied the effect of this protein on modulating the immune response of goat peripheral blood mononuclear cells (PBMCs) and monocytes. Immunohistochemical tests verified that the HCETFα protein was localized mainly in the intestinal wall and on the body surface of worms. Immunoblot analysis revealed that rHCETFα was recognized by the serum of goats artificially infected with H. contortus. Immunofluorescence analysis indicated that rHCETFα bound to the surface of PBMCs. rHCETFα was co-incubated with goat PBMCs to observe the immunomodulatory effects exerted by HCETFα on proliferation, apoptosis, cytokine secretion and nitric oxide (NO) production. The results showed that rHCETFα suppressed the proliferation of goat PBMCs stimulated by concanavalin A and induced apoptosis in goat PBMCs. After rHCETFα exposure, IL-2, IL-4, IL-17A and TNF-α expression was markedly reduced, whereas secretion of TGF-β1 was significantly elevated, in goat PBMCs. Moreover, rHCETFα up-regulated NO production in a dose-dependent manner. FITC-dextran internalization assays showed that rHCETFα inhibited phagocytosis of goat monocytes. These results elucidate the interaction between parasites and hosts at the molecular level, suggest a possible immunomodulatory target and contribute to the search for innovative proteins that may be candidate targets for drugs and vaccines." @default.
- W3096103422 created "2020-11-09" @default.
- W3096103422 creator A5006901857 @default.
- W3096103422 creator A5015838262 @default.
- W3096103422 creator A5033133176 @default.
- W3096103422 creator A5037765953 @default.
- W3096103422 creator A5038699958 @default.
- W3096103422 creator A5047841885 @default.
- W3096103422 creator A5066281981 @default.
- W3096103422 creator A5088929130 @default.
- W3096103422 date "2020-12-01" @default.
- W3096103422 modified "2023-10-17" @default.
- W3096103422 title "Modulatory functions of recombinant electron transfer flavoprotein α subunit protein from Haemonchus contortus on goat immune cells in vitro" @default.
- W3096103422 cites W1481122512 @default.
- W3096103422 cites W1488931513 @default.
- W3096103422 cites W1560571961 @default.
- W3096103422 cites W1577082055 @default.
- W3096103422 cites W1583052190 @default.
- W3096103422 cites W1628123357 @default.
- W3096103422 cites W1819486833 @default.
- W3096103422 cites W1906153342 @default.
- W3096103422 cites W1943769738 @default.
- W3096103422 cites W1965859404 @default.
- W3096103422 cites W1968169978 @default.
- W3096103422 cites W1978594839 @default.
- W3096103422 cites W1979618663 @default.
- W3096103422 cites W1994069021 @default.
- W3096103422 cites W1998356414 @default.
- W3096103422 cites W2004837100 @default.
- W3096103422 cites W2005886572 @default.
- W3096103422 cites W2014882563 @default.
- W3096103422 cites W2021454776 @default.
- W3096103422 cites W2021926390 @default.
- W3096103422 cites W2022828031 @default.
- W3096103422 cites W2028117344 @default.
- W3096103422 cites W2028944525 @default.
- W3096103422 cites W2030140262 @default.
- W3096103422 cites W2032240345 @default.
- W3096103422 cites W2045964805 @default.
- W3096103422 cites W2054009606 @default.
- W3096103422 cites W2058131526 @default.
- W3096103422 cites W2062529150 @default.
- W3096103422 cites W2084015888 @default.
- W3096103422 cites W2086842750 @default.
- W3096103422 cites W2093897672 @default.
- W3096103422 cites W2094016763 @default.
- W3096103422 cites W2100769504 @default.
- W3096103422 cites W2103348129 @default.
- W3096103422 cites W2115130133 @default.
- W3096103422 cites W2121454964 @default.
- W3096103422 cites W2125227434 @default.
- W3096103422 cites W2126723350 @default.
- W3096103422 cites W2129514426 @default.
- W3096103422 cites W2130988133 @default.
- W3096103422 cites W2133300351 @default.
- W3096103422 cites W2133522068 @default.
- W3096103422 cites W2134530059 @default.
- W3096103422 cites W2135449563 @default.
- W3096103422 cites W2135530598 @default.
- W3096103422 cites W2139428609 @default.
- W3096103422 cites W2152350632 @default.
- W3096103422 cites W2154354014 @default.
- W3096103422 cites W2157026876 @default.
- W3096103422 cites W2159982153 @default.
- W3096103422 cites W2161733022 @default.
- W3096103422 cites W2163045345 @default.
- W3096103422 cites W2164375615 @default.
- W3096103422 cites W2170257554 @default.
- W3096103422 cites W2171105241 @default.
- W3096103422 cites W2396475159 @default.
- W3096103422 cites W2505331358 @default.
- W3096103422 cites W2597465574 @default.
- W3096103422 cites W2606252470 @default.
- W3096103422 cites W2723926921 @default.
- W3096103422 cites W2743797285 @default.
- W3096103422 cites W2746313985 @default.
- W3096103422 cites W2755966516 @default.
- W3096103422 doi "https://doi.org/10.1016/j.vetpar.2020.109300" @default.
- W3096103422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33152677" @default.
- W3096103422 hasPublicationYear "2020" @default.
- W3096103422 type Work @default.
- W3096103422 sameAs 3096103422 @default.
- W3096103422 citedByCount "1" @default.
- W3096103422 countsByYear W30961034222022 @default.
- W3096103422 crossrefType "journal-article" @default.
- W3096103422 hasAuthorship W3096103422A5006901857 @default.
- W3096103422 hasAuthorship W3096103422A5015838262 @default.
- W3096103422 hasAuthorship W3096103422A5033133176 @default.
- W3096103422 hasAuthorship W3096103422A5037765953 @default.
- W3096103422 hasAuthorship W3096103422A5038699958 @default.
- W3096103422 hasAuthorship W3096103422A5047841885 @default.
- W3096103422 hasAuthorship W3096103422A5066281981 @default.
- W3096103422 hasAuthorship W3096103422A5088929130 @default.
- W3096103422 hasConcept C104317684 @default.
- W3096103422 hasConcept C137061746 @default.
- W3096103422 hasConcept C153911025 @default.
- W3096103422 hasConcept C160448771 @default.
- W3096103422 hasConcept C165901193 @default.