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• W3096303362 endingPage "116590" @default.
• W3096303362 startingPage "116590" @default.
• W3096303362 abstract "Cysts and (oo)cysts are the infective forms of parasitic protozoa, as Giardia and Cryptosporidium, which are widespread and associated to worldwide waterborne diseases outbreaks. These microorganisms pose a challenge to public health, as they are resistant to conventional disinfection methods, which make them important parameters when evaluating inactivation efficiency. However, when (oo)cysts are targets, it is challenging to infer inactivation efficacy, as it may require infectivity tests that are not often an option for laboratory routine analysis. In this scene, (oo)cyst viability based on induced excystation, membrane integrity and enzyme activity evaluated by dye inclusion and/or exclusion, as well as fluorescence reduction consist on microscopy-based techniques that may be options to estimate inactivation in the environmental context. This scoping review presents applications, advantages and limitations of these methodologies for viability assessment, in order to shed light on the (oo)cyst viability topic and provide insight strategies for choosing protocols in the environmental and sanitation field, in laboratory applications and novel research." @default.
• W3096303362 created "2020-11-09" @default.
• W3096303362 creator A5057858856 @default.
• W3096303362 creator A5082784922 @default.
• W3096303362 date "2021-02-01" @default.
• W3096303362 modified "2023-10-16" @default.
• W3096303362 title "Cryptosporidium spp. and Giardia spp. (oo)cysts as target-organisms in sanitation and environmental monitoring: A review in microscopy-based viability assays" @default.
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• W3096303362 doi "https://doi.org/10.1016/j.watres.2020.116590" @default.

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