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- W3096314717 abstract "Fluoroquinolones and cephalosporins are critically important antimicrobial classes for both human and veterinary medicine. We previously found a drastic increase in enrofloxacin resistance in clinical Escherichia coli isolates collected from diseased pigs from the United States over 10 years (2006 to 2016). However, the genetic determinants responsible for this increase have yet to be determined. The aim of the present study was to identify and characterize the genetic basis of resistance against fluoroquinolones (enrofloxacin) and extended-spectrum cephalosporins (ceftiofur) in swine E. coli isolates using whole-genome sequencing (WGS). blaCMY-2 (carried by IncA/C2, IncI1, and IncI2 plasmids), blaCTX-M (carried by IncF, IncHI2, and IncN plasmids), and blaSHV-12 (carried by IncHI2 plasmids) genes were present in 87 (82.1%), 19 (17.9%), and 3 (2.83%) of the 106 ceftiofur-resistant isolates, respectively. Of the 110 enrofloxacin-resistant isolates, 90 (81.8%) had chromosomal mutations in gyrA, gyrB, parA, and parC genes. Plasmid-mediated quinolone resistance genes [qnrB77, qnrB2, qnrS1, qnrS2, and aac-(6)-lb'-cr] borne on ColE, IncQ2, IncN, IncF, and IncHI2 plasmids were present in 24 (21.8%) of the enrofloxacin-resistant isolates. Virulent IncF plasmids present in swine E. coli isolates were highly similar to epidemic plasmids identified globally. High-risk E. coli clones, such as ST744, ST457, ST131, ST69, ST10, ST73, ST410, ST12, ST127, ST167, ST58, ST88, ST617, ST23, etc., were also found in the U.S. swine population. Additionally, the colistin resistance gene (mcr-9) was present in several isolates. This study adds valuable information regarding resistance to critical antimicrobials with implications for both animal and human health.IMPORTANCE Understanding the genetic mechanisms conferring resistance is critical to design informed control and preventive measures, particularly when involving critically important antimicrobial classes such as extended-spectrum cephalosporins and fluoroquinolones. The genetic determinants of extended-spectrum cephalosporin and fluoroquinolone resistance were highly diverse, with multiple plasmids, insertion sequences, and genes playing key roles in mediating resistance in swine Escherichia coli Plasmids assembled in this study are known to be disseminated globally in both human and animal populations and environmental samples, and E. coli in pigs might be part of a global reservoir of key antimicrobial resistance (AMR) elements. Virulent plasmids found in this study have been shown to confer fitness advantages to pathogenic E. coli strains. The presence of international, high-risk zoonotic clones provides worrisome evidence that resistance in swine isolates may have indirect public health implications, and the swine population as a reservoir for these high-risk clones should be continuously monitored." @default.
- W3096314717 created "2020-11-09" @default.
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- W3096314717 date "2020-10-28" @default.
- W3096314717 modified "2023-10-18" @default.
- W3096314717 title "Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States" @default.
- W3096314717 cites W1951188525 @default.
- W3096314717 cites W1977678315 @default.
- W3096314717 cites W1987126683 @default.
- W3096314717 cites W1989342781 @default.
- W3096314717 cites W1990352146 @default.
- W3096314717 cites W1992336491 @default.
- W3096314717 cites W1992668798 @default.
- W3096314717 cites W2008412369 @default.
- W3096314717 cites W2018206080 @default.
- W3096314717 cites W2025021478 @default.
- W3096314717 cites W2027875143 @default.
- W3096314717 cites W2031226004 @default.
- W3096314717 cites W2032499375 @default.
- W3096314717 cites W2044062578 @default.
- W3096314717 cites W2046926760 @default.
- W3096314717 cites W2056163574 @default.
- W3096314717 cites W2058415702 @default.
- W3096314717 cites W2070845246 @default.
- W3096314717 cites W2072455391 @default.
- W3096314717 cites W2091030812 @default.
- W3096314717 cites W2092488799 @default.
- W3096314717 cites W2095824770 @default.
- W3096314717 cites W2096093282 @default.
- W3096314717 cites W2098957487 @default.
- W3096314717 cites W2108234281 @default.
- W3096314717 cites W2111281355 @default.
- W3096314717 cites W2113308481 @default.
- W3096314717 cites W2116233885 @default.
- W3096314717 cites W2116984251 @default.
- W3096314717 cites W2117043113 @default.
- W3096314717 cites W2120902911 @default.
- W3096314717 cites W2121997159 @default.
- W3096314717 cites W2130010389 @default.
- W3096314717 cites W2131271579 @default.
- W3096314717 cites W2134852597 @default.
- W3096314717 cites W2136966641 @default.
- W3096314717 cites W2141052558 @default.
- W3096314717 cites W2141241487 @default.
- W3096314717 cites W2143325926 @default.
- W3096314717 cites W2150418059 @default.
- W3096314717 cites W2153325555 @default.
- W3096314717 cites W2154315063 @default.
- W3096314717 cites W2155858551 @default.
- W3096314717 cites W2158669671 @default.
- W3096314717 cites W2160177274 @default.
- W3096314717 cites W2161495927 @default.
- W3096314717 cites W2163324254 @default.
- W3096314717 cites W2169894107 @default.
- W3096314717 cites W2170883440 @default.
- W3096314717 cites W2178556812 @default.
- W3096314717 cites W2207760002 @default.
- W3096314717 cites W2263256665 @default.
- W3096314717 cites W2298346855 @default.
- W3096314717 cites W2346143774 @default.
- W3096314717 cites W2406191933 @default.
- W3096314717 cites W2478596546 @default.
- W3096314717 cites W2522537319 @default.
- W3096314717 cites W2523485632 @default.
- W3096314717 cites W2560067869 @default.
- W3096314717 cites W2582206824 @default.
- W3096314717 cites W2582648685 @default.
- W3096314717 cites W2586947962 @default.
- W3096314717 cites W2592954192 @default.
- W3096314717 cites W2595296766 @default.
- W3096314717 cites W2610564464 @default.
- W3096314717 cites W2619325951 @default.
- W3096314717 cites W2725324739 @default.
- W3096314717 cites W2742299527 @default.
- W3096314717 cites W2744355000 @default.
- W3096314717 cites W2746809581 @default.
- W3096314717 cites W2752246619 @default.
- W3096314717 cites W2753422859 @default.
- W3096314717 cites W2767117391 @default.
- W3096314717 cites W2778143559 @default.
- W3096314717 cites W2782085461 @default.
- W3096314717 cites W2786095190 @default.
- W3096314717 cites W2791729250 @default.
- W3096314717 cites W2794070260 @default.
- W3096314717 cites W2794458297 @default.
- W3096314717 cites W2800768460 @default.
- W3096314717 cites W2803637930 @default.
- W3096314717 cites W2883436641 @default.
- W3096314717 cites W2885680427 @default.
- W3096314717 cites W2889332465 @default.
- W3096314717 cites W2902380726 @default.