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• W3096384295 endingPage "183501" @default.
• W3096384295 startingPage "183501" @default.
• W3096384295 abstract "We have employed the peptide framework of GWALP23 (acetyl-GGALWLALALALALALALWLAGA-amide) to examine the orientation, dynamics and pH dependence of peptides having buried single or pairs of histidine residues. When residue L8 is substituted to yield GWALP23-H8, acetyl-GGALWLAH8ALALALALALWLAGA-amide, the deuterium NMR spectra of 2H-labeled core alanine residues reveal a helix that occupies a single transmembrane orientation in DLPC, or in DMPC at low pH, yet shows multiple states at higher pH or in bilayers of DOPC. Moreover, a single histidine at position 8 or 16 in the GWALP23 framework is sensitive to pH. Titration points are observed near pH 3.5 for the deprotonation of H8 in lipid bilayers of DLPC or DMPC, and for H16 in DOPC. When residues L8 and L16 both are substituted to yield GWALP23-H8,16, the 2H NMR spectra show, interestingly, no titration dependence from pH 2–8, yet bilayer thickness-dependent orientation differences. The helix with H8 and H16 is found to adopt a transmembrane orientation in thin bilayers of DLPC, a combination of transmembrane and surface orientations in DMPC, and then a complete transition to a surface bound orientation in the thicker DPoPC and DOPC lipid bilayers. In the surface orientations, alanine A7 no longer fits within the core helix. These results along with previous studies with different locations of histidine residues suggest that lipid hydrophobic thickness is a first determinant and pH a second determinant for the helical orientation, along with possible side-chain snorkeling, when the His residues are incorporated into the hydrophobic region of a lipid membrane-associated helix." @default.
• W3096384295 created "2020-11-09" @default.
• W3096384295 creator A5023132045 @default.
• W3096384295 creator A5044385830 @default.
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• W3096384295 creator A5083480671 @default.
• W3096384295 date "2021-01-01" @default.
• W3096384295 modified "2023-09-27" @default.
• W3096384295 title "Examination of pH dependency and orientation differences of membrane spanning alpha helices carrying a single or pair of buried histidine residues" @default.
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• W3096384295 doi "https://doi.org/10.1016/j.bbamem.2020.183501" @default.
• W3096384295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33130099" @default.
• W3096384295 hasPublicationYear "2021" @default.
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