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• W3096409475 abstract "Indole is a major metabolite of tryptophan, which plays an important role in the intestinal microecological balance and human physiological activities. The determination of indole becomes important for its researches. So, it is urgent to establish a sensitive and cost-effective method for indole detection. Herein, a sensitive electrochemical method was constructed to determine the concentration of indole using screen-printed carbon electrode (SPCE) with the signal amplification strategy by gold/iron-oxide composite nanoparticles (Au/Fe3O4). Au/Fe3O4 nanoparticles were successfully synthesized under the irradiation by high-energy electron beams. 4-aminothiophenol (4-ATP) was connected to Au/Fe3O4 via Au-S bond. And then NaNO2 reacted with 4-ATP to form the azo bond, which could form the final product of Au/Fe3O4@ATP-azo-indole by the coupling reaction. Thus, the concentration of indole was detected by the electrochemical signal produced by Au/Fe3O4@ATP-azo-indole indirectly. The detection sensitivity was greatly improved by the large specific surface area provided by Au/Fe3O4 after the modification. The linear range of indole was from 0.50 to 120.00 μg L−1 and the limit of detection (LOD) was as low as 0.10 μg L−1 (S/N = 3). Furthermore, the developed method exhibited acceptable intra-day and inter-day precisions with the coefficient of variations (CV) less than 4.9% and 8.2%, respectively. And the recoveries were from 97.2% to 105.4%. An innovative, sensitive, cost-effective method was established for indole determination in human plasma matrix in this manuscript, which provides a promising way for indole detection in conventional laboratories." @default.
• W3096409475 created "2020-11-09" @default.
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• W3096409475 date "2021-01-01" @default.
• W3096409475 modified "2023-09-25" @default.
• W3096409475 title "A sensitive electrochemical method for indole based on the signal amplification strategy by gold/iron-oxide composite nanoparticles" @default.
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• W3096409475 doi "https://doi.org/10.1016/j.aca.2020.10.055" @default.
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