FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096571719> ?p ?o ?g. }

• W3096571719 endingPage "114013" @default.
• W3096571719 startingPage "114013" @default.
• W3096571719 abstract "The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) emergence in 2003 introduced the first serious human coronavirus pathogen to an unprepared world. To control emerging viruses, existing successful anti(retro)viral therapies can inspire antiviral strategies, as conserved viral enzymes (eg., viral proteases and RNA-dependent RNA polymerases) represent targets of choice. Since 2003, much effort has been expended in the characterization of the SARS-CoV replication/transcription machinery. Until recently, a pure and highly active preparation of SARS-CoV recombinant RNA synthesis machinery was not available, impeding target-based high throughput screening of drug candidates against this viral family. The current Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) pandemic revealed a new pathogen whose RNA synthesis machinery is highly (>96 % aa identity) homologous to SARS-CoV. This phylogenetic relatedness highlights the potential use of conserved replication enzymes to discover inhibitors against this significant pathogen, which in turn, contributes to scientific preparedness against emerging viruses. Here, we report the use of a purified and highly active SARS-CoV replication/transcription complex (RTC) to set-up a high-throughput screening of Coronavirus RNA synthesis inhibitors. The screening of a small (1520 compounds) chemical library of FDA-approved drugs demonstrates the robustness of our assay and will allow to speed-up drug discovery against the SARS-CoV-2." @default.
• W3096571719 created "2020-11-09" @default.
• W3096571719 creator A5004072834 @default.
• W3096571719 creator A5019210804 @default.
• W3096571719 creator A5038176175 @default.
• W3096571719 creator A5039783946 @default.
• W3096571719 creator A5067709711 @default.
• W3096571719 creator A5072176046 @default.
• W3096571719 creator A5075862078 @default.
• W3096571719 date "2021-02-01" @default.
• W3096571719 modified "2023-10-17" @default.
• W3096571719 title "A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex" @default.
• W3096571719 cites W1967505975 @default.
• W3096571719 cites W2140943911 @default.
• W3096571719 cites W2159857626 @default.
• W3096571719 cites W2206069791 @default.
• W3096571719 cites W2791400386 @default.
• W3096571719 cites W2914796231 @default.
• W3096571719 cites W2945440984 @default.
• W3096571719 cites W2945682340 @default.
• W3096571719 cites W3003217347 @default.
• W3096571719 cites W3004280078 @default.
• W3096571719 cites W3010795344 @default.
• W3096571719 cites W3015429854 @default.
• W3096571719 cites W3015472194 @default.
• W3096571719 cites W3025511956 @default.
• W3096571719 doi "https://doi.org/10.1016/j.jviromet.2020.114013" @default.
• W3096571719 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7833800" @default.
• W3096571719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33166547" @default.
• W3096571719 hasPublicationYear "2021" @default.
• W3096571719 type Work @default.
• W3096571719 sameAs 3096571719 @default.
• W3096571719 citedByCount "15" @default.
• W3096571719 countsByYear W30965717192021 @default.
• W3096571719 countsByYear W30965717192022 @default.
• W3096571719 countsByYear W30965717192023 @default.
• W3096571719 crossrefType "journal-article" @default.
• W3096571719 hasAuthorship W3096571719A5004072834 @default.
• W3096571719 hasAuthorship W3096571719A5019210804 @default.
• W3096571719 hasAuthorship W3096571719A5038176175 @default.
• W3096571719 hasAuthorship W3096571719A5039783946 @default.
• W3096571719 hasAuthorship W3096571719A5067709711 @default.
• W3096571719 hasAuthorship W3096571719A5072176046 @default.
• W3096571719 hasAuthorship W3096571719A5075862078 @default.
• W3096571719 hasBestOaLocation W30965717191 @default.
• W3096571719 hasConcept C104317684 @default.
• W3096571719 hasConcept C138885662 @default.
• W3096571719 hasConcept C140704245 @default.
• W3096571719 hasConcept C142724271 @default.
• W3096571719 hasConcept C159047783 @default.
• W3096571719 hasConcept C179926584 @default.
• W3096571719 hasConcept C2522874641 @default.
• W3096571719 hasConcept C2777648638 @default.
• W3096571719 hasConcept C2779134260 @default.
• W3096571719 hasConcept C3008058167 @default.
• W3096571719 hasConcept C41258723 @default.
• W3096571719 hasConcept C41895202 @default.
• W3096571719 hasConcept C524204448 @default.
• W3096571719 hasConcept C54355233 @default.
• W3096571719 hasConcept C60644358 @default.
• W3096571719 hasConcept C67705224 @default.
• W3096571719 hasConcept C70721500 @default.
• W3096571719 hasConcept C71924100 @default.
• W3096571719 hasConcept C74187038 @default.
• W3096571719 hasConcept C86803240 @default.
• W3096571719 hasConceptScore W3096571719C104317684 @default.
• W3096571719 hasConceptScore W3096571719C138885662 @default.
• W3096571719 hasConceptScore W3096571719C140704245 @default.
• W3096571719 hasConceptScore W3096571719C142724271 @default.
• W3096571719 hasConceptScore W3096571719C159047783 @default.
• W3096571719 hasConceptScore W3096571719C179926584 @default.
• W3096571719 hasConceptScore W3096571719C2522874641 @default.
• W3096571719 hasConceptScore W3096571719C2777648638 @default.
• W3096571719 hasConceptScore W3096571719C2779134260 @default.
• W3096571719 hasConceptScore W3096571719C3008058167 @default.
• W3096571719 hasConceptScore W3096571719C41258723 @default.
• W3096571719 hasConceptScore W3096571719C41895202 @default.
• W3096571719 hasConceptScore W3096571719C524204448 @default.
• W3096571719 hasConceptScore W3096571719C54355233 @default.
• W3096571719 hasConceptScore W3096571719C60644358 @default.
• W3096571719 hasConceptScore W3096571719C67705224 @default.
• W3096571719 hasConceptScore W3096571719C70721500 @default.
• W3096571719 hasConceptScore W3096571719C71924100 @default.
• W3096571719 hasConceptScore W3096571719C74187038 @default.
• W3096571719 hasConceptScore W3096571719C86803240 @default.
• W3096571719 hasFunder F4320320883 @default.
• W3096571719 hasLocation W30965717191 @default.
• W3096571719 hasLocation W30965717192 @default.
• W3096571719 hasLocation W30965717193 @default.
• W3096571719 hasLocation W30965717194 @default.
• W3096571719 hasLocation W30965717195 @default.
• W3096571719 hasOpenAccess W3096571719 @default.
• W3096571719 hasPrimaryLocation W30965717191 @default.
• W3096571719 hasRelatedWork W1972327390 @default.
• W3096571719 hasRelatedWork W2005006576 @default.
• W3096571719 hasRelatedWork W2043189073 @default.
• W3096571719 hasRelatedWork W2061997414 @default.
• W3096571719 hasRelatedWork W2072789690 @default.

• next