Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096609940> ?p ?o ?g. }
- W3096609940 abstract "The emerging pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused social and economic disruption worldwide, infecting over 9.0 million people and killing over 469 000 by 24 June 2020. Unfortunately, no vaccine or antiviral drug that completely eliminates the transmissible disease coronavirus disease 2019 (COVID-19) has been developed to date. Given that coronavirus nonstructural protein 1 (nsp1) is a good target for attenuated vaccines, it is of great significance to explore the detailed characteristics of SARS-CoV-2 nsp1. Here, we first confirmed that SARS-CoV-2 nsp1 had a conserved function similar to that of SARS-CoV nsp1 in inhibiting host-protein synthesis and showed greater inhibition efficiency, as revealed by ribopuromycylation and Renilla luciferase (Rluc) reporter assays. Specifically, bioinformatics and biochemical experiments showed that by interacting with 40S ribosomal subunit, the lysine located at amino acid 164 (K164) was the key residue that enabled SARS-CoV-2 nsp1 to suppress host gene expression. Furthermore, as an inhibitor of host-protein expression, SARS-CoV-2 nsp1 contributed to cell-cycle arrest in G0/G1 phase, which might provide a favourable environment for virus production. Taken together, this research uncovered the detailed mechanism by which SARS-CoV-2 nsp1 K164 inhibited host gene expression, laying the foundation for the development of attenuated vaccines based on nsp1 modification." @default.
- W3096609940 created "2020-11-09" @default.
- W3096609940 creator A5019061615 @default.
- W3096609940 creator A5043807389 @default.
- W3096609940 creator A5058957336 @default.
- W3096609940 creator A5065459950 @default.
- W3096609940 creator A5068523570 @default.
- W3096609940 creator A5073518856 @default.
- W3096609940 date "2021-01-01" @default.
- W3096609940 modified "2023-10-16" @default.
- W3096609940 title "Lysine 164 is critical for SARS-CoV-2 Nsp1 inhibition of host gene expression" @default.
- W3096609940 cites W1754426546 @default.
- W3096609940 cites W1967021382 @default.
- W3096609940 cites W1985346742 @default.
- W3096609940 cites W1992509320 @default.
- W3096609940 cites W1999050752 @default.
- W3096609940 cites W2005442019 @default.
- W3096609940 cites W2017989844 @default.
- W3096609940 cites W2043726848 @default.
- W3096609940 cites W2053186308 @default.
- W3096609940 cites W2057987200 @default.
- W3096609940 cites W2068070201 @default.
- W3096609940 cites W2079255671 @default.
- W3096609940 cites W2081997160 @default.
- W3096609940 cites W2119628526 @default.
- W3096609940 cites W2132260239 @default.
- W3096609940 cites W2134061616 @default.
- W3096609940 cites W2156095183 @default.
- W3096609940 cites W2166867592 @default.
- W3096609940 cites W2218044799 @default.
- W3096609940 cites W2233577420 @default.
- W3096609940 cites W2339116108 @default.
- W3096609940 cites W2470646526 @default.
- W3096609940 cites W2586577766 @default.
- W3096609940 cites W2594362193 @default.
- W3096609940 cites W2735084243 @default.
- W3096609940 cites W2751812100 @default.
- W3096609940 cites W2763547223 @default.
- W3096609940 cites W2800793809 @default.
- W3096609940 cites W2885392667 @default.
- W3096609940 cites W2887342775 @default.
- W3096609940 cites W2902072701 @default.
- W3096609940 cites W2964664812 @default.
- W3096609940 cites W2998476335 @default.
- W3096609940 cites W3001465255 @default.
- W3096609940 cites W3003664710 @default.
- W3096609940 cites W3004280078 @default.
- W3096609940 cites W3009335299 @default.
- W3096609940 cites W3010238729 @default.
- W3096609940 cites W3011488280 @default.
- W3096609940 cites W3012764845 @default.
- W3096609940 cites W3013093478 @default.
- W3096609940 cites W3013800395 @default.
- W3096609940 cites W3014067025 @default.
- W3096609940 cites W3016885766 @default.
- W3096609940 cites W3017186084 @default.
- W3096609940 cites W3018540129 @default.
- W3096609940 cites W3020196527 @default.
- W3096609940 cites W3022917947 @default.
- W3096609940 cites W3023609275 @default.
- W3096609940 cites W3043427328 @default.
- W3096609940 cites W3165656738 @default.
- W3096609940 doi "https://doi.org/10.1099/jgv.0.001513" @default.
- W3096609940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8116783" @default.
- W3096609940 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33151142" @default.
- W3096609940 hasPublicationYear "2021" @default.
- W3096609940 type Work @default.
- W3096609940 sameAs 3096609940 @default.
- W3096609940 citedByCount "19" @default.
- W3096609940 countsByYear W30966099402021 @default.
- W3096609940 countsByYear W30966099402022 @default.
- W3096609940 countsByYear W30966099402023 @default.
- W3096609940 crossrefType "journal-article" @default.
- W3096609940 hasAuthorship W3096609940A5019061615 @default.
- W3096609940 hasAuthorship W3096609940A5043807389 @default.
- W3096609940 hasAuthorship W3096609940A5058957336 @default.
- W3096609940 hasAuthorship W3096609940A5065459950 @default.
- W3096609940 hasAuthorship W3096609940A5068523570 @default.
- W3096609940 hasAuthorship W3096609940A5073518856 @default.
- W3096609940 hasBestOaLocation W30966099402 @default.
- W3096609940 hasConcept C104317684 @default.
- W3096609940 hasConcept C140704245 @default.
- W3096609940 hasConcept C142724271 @default.
- W3096609940 hasConcept C150194340 @default.
- W3096609940 hasConcept C159047783 @default.
- W3096609940 hasConcept C2522874641 @default.
- W3096609940 hasConcept C25897203 @default.
- W3096609940 hasConcept C2776460866 @default.
- W3096609940 hasConcept C2777648638 @default.
- W3096609940 hasConcept C2779134260 @default.
- W3096609940 hasConcept C3008058167 @default.
- W3096609940 hasConcept C524204448 @default.
- W3096609940 hasConcept C54355233 @default.
- W3096609940 hasConcept C71924100 @default.
- W3096609940 hasConcept C86803240 @default.
- W3096609940 hasConcept C89423630 @default.
- W3096609940 hasConceptScore W3096609940C104317684 @default.
- W3096609940 hasConceptScore W3096609940C140704245 @default.
- W3096609940 hasConceptScore W3096609940C142724271 @default.
- W3096609940 hasConceptScore W3096609940C150194340 @default.