FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096756903> ?p ?o ?g. }

• W3096756903 endingPage "1" @default.
• W3096756903 startingPage "1" @default.
• W3096756903 abstract "To investigate the major organelles of the retinal pigment epithelium (RPE) in wild-type (WT, control) mice and their changes in pigmented Abca4 knockout (Abca4-/-) mice with in situ morphologic, spatial, and spectral characterization of live ex vivo flat-mounted RPE using multicolor confocal fluorescence microscopy (MCFM).In situ imaging of RPE flat-mounts of agouti Abca4-/- (129S4), agouti WT (129S1/SvlmJ) controls, and B6 albino mice (C57BL/6J-Tyrc-Brd) was performed with a Nikon A1 confocal microscope. High-resolution confocal image z-stacks of the RPE cell mosaic were acquired with four different excitation wavelengths (405 nm, 488 nm, 561 nm, and 640 nm). The autofluorescence images of RPE, including voxel-by-voxel emission spectra, were acquired and processed with Nikon NIS-AR Elements software.The 3-dimensional multicolor confocal images provided a detailed visualization of the RPE cell mosaic, including its melanosomes and lipofuscin granules, and their varying characteristics in the different mice strains. The autofluorescence spectra, spatial distribution, and morphologic features of melanosomes and lipofuscin granules were measured. Increased numbers of lipofuscin and reduced numbers of melanosomes were observed in the RPE of Abca4-/- mice relative to controls.A detailed assessment of the RPE autofluorescent granules and their changes ex vivo was possible with MCFM. For all excitation wavelengths, autofluorescence from the RPE cells was predominantly contributed by lipofuscin granules, while melanosomes were found to be essentially nonfluorescent. The red shift of the emission peak confirmed the presence of multiple chromophores within lipofuscin granules. The elevated autofluorescence levels in Abca4-/- mice correlated well with the increased number of lipofuscin granules." @default.
• W3096756903 created "2020-11-09" @default.
• W3096756903 creator A5016179646 @default.
• W3096756903 creator A5037070406 @default.
• W3096756903 creator A5044266730 @default.
• W3096756903 creator A5049505771 @default.
• W3096756903 creator A5066376581 @default.
• W3096756903 creator A5077412073 @default.
• W3096756903 creator A5081788525 @default.
• W3096756903 date "2020-11-20" @default.
• W3096756903 modified "2023-09-29" @default.
• W3096756903 title "In Situ Morphologic and Spectral Characterization of Retinal Pigment Epithelium Organelles in Mice Using Multicolor Confocal Fluorescence Imaging" @default.
• W3096756903 cites W1285309 @default.
• W3096756903 cites W132229772 @default.
• W3096756903 cites W133307128 @default.
• W3096756903 cites W1523693806 @default.
• W3096756903 cites W1599897764 @default.
• W3096756903 cites W195065889 @default.
• W3096756903 cites W1977736972 @default.
• W3096756903 cites W1984815704 @default.
• W3096756903 cites W1989558725 @default.
• W3096756903 cites W1994172606 @default.
• W3096756903 cites W2014419387 @default.
• W3096756903 cites W2021234912 @default.
• W3096756903 cites W2023768917 @default.
• W3096756903 cites W2030787754 @default.
• W3096756903 cites W2038113411 @default.
• W3096756903 cites W2059022308 @default.
• W3096756903 cites W2060448170 @default.
• W3096756903 cites W2070586687 @default.
• W3096756903 cites W2088740316 @default.
• W3096756903 cites W2094857141 @default.
• W3096756903 cites W2109494581 @default.
• W3096756903 cites W2123215356 @default.
• W3096756903 cites W2147841957 @default.
• W3096756903 cites W2160226180 @default.
• W3096756903 cites W2173345696 @default.
• W3096756903 cites W2175406334 @default.
• W3096756903 cites W2273142706 @default.
• W3096756903 cites W2401647389 @default.
• W3096756903 cites W2410161487 @default.
• W3096756903 cites W2411031570 @default.
• W3096756903 cites W2474213933 @default.
• W3096756903 cites W2604383700 @default.
• W3096756903 cites W2808520358 @default.
• W3096756903 cites W2884031436 @default.
• W3096756903 cites W2900433461 @default.
• W3096756903 cites W2954259932 @default.
• W3096756903 cites W2981891048 @default.
• W3096756903 cites W2997004229 @default.
• W3096756903 cites W4231240273 @default.
• W3096756903 cites W4245829749 @default.
• W3096756903 cites W4248884067 @default.
• W3096756903 cites W60870114 @default.
• W3096756903 doi "https://doi.org/10.1167/iovs.61.13.1" @default.
• W3096756903 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7645167" @default.
• W3096756903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33137194" @default.
• W3096756903 hasPublicationYear "2020" @default.
• W3096756903 type Work @default.
• W3096756903 sameAs 3096756903 @default.
• W3096756903 citedByCount "15" @default.
• W3096756903 countsByYear W30967569032021 @default.
• W3096756903 countsByYear W30967569032022 @default.
• W3096756903 countsByYear W30967569032023 @default.
• W3096756903 crossrefType "journal-article" @default.
• W3096756903 hasAuthorship W3096756903A5016179646 @default.
• W3096756903 hasAuthorship W3096756903A5037070406 @default.
• W3096756903 hasAuthorship W3096756903A5044266730 @default.
• W3096756903 hasAuthorship W3096756903A5049505771 @default.
• W3096756903 hasAuthorship W3096756903A5066376581 @default.
• W3096756903 hasAuthorship W3096756903A5077412073 @default.
• W3096756903 hasAuthorship W3096756903A5081788525 @default.
• W3096756903 hasBestOaLocation W30967569031 @default.
• W3096756903 hasConcept C104317684 @default.
• W3096756903 hasConcept C107538193 @default.
• W3096756903 hasConcept C120665830 @default.
• W3096756903 hasConcept C121332964 @default.
• W3096756903 hasConcept C12554922 @default.
• W3096756903 hasConcept C127716648 @default.
• W3096756903 hasConcept C136009344 @default.
• W3096756903 hasConcept C169274487 @default.
• W3096756903 hasConcept C179687394 @default.
• W3096756903 hasConcept C185592680 @default.
• W3096756903 hasConcept C186198217 @default.
• W3096756903 hasConcept C2778024200 @default.
• W3096756903 hasConcept C2779178360 @default.
• W3096756903 hasConcept C2779460726 @default.
• W3096756903 hasConcept C2780827179 @default.
• W3096756903 hasConcept C55493867 @default.
• W3096756903 hasConcept C86803240 @default.
• W3096756903 hasConcept C91881484 @default.
• W3096756903 hasConcept C94435474 @default.
• W3096756903 hasConcept C95444343 @default.
• W3096756903 hasConceptScore W3096756903C104317684 @default.
• W3096756903 hasConceptScore W3096756903C107538193 @default.
• W3096756903 hasConceptScore W3096756903C120665830 @default.
• W3096756903 hasConceptScore W3096756903C121332964 @default.
• W3096756903 hasConceptScore W3096756903C12554922 @default.

• next