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• W3096807388 abstract "Exosomes involved in tumor-specific processes display excellent potential in the early diagnosis of cancer. Herein, a highly sensitive plasmonic colorimetric biosensor was proposed for exosome quantification. The sensing strategy mainly includes two steps: exosome-triggered competitive reaction and etching of gold nanobipyramid@MnO2 nanosheet nanostructures (Au NBP@MnO2 NSs). A competitive reaction between exosomes and placeholder chains induced by exosomes can translate the signal of exosomes into the amount of alkaline phosphatase, which simplifies the experimental process and amplifies the signal. The etching of Au NBP@MnO2 NSs by ascorbic acid generated from the hydrolysis of l-ascorbic acid 2-phosphate by alkaline phosphatase changes the refractive index of Au NBPs, accompanied by the blue shift of the longitudinal localized surface plasmon resonance peak. Profiting from the signal amplification of the competitive reaction and superior refractive index sensitivity of colorimetric substrates, this protocol exhibits high sensitivity toward exosomes within 8.5 × 102 to 8.5 × 104 particles μL-1, along with a detection limit of 1.35 × 102 particles μL-1, which is more sensitive than previously reported colorimetric methods. In addition, a sensitive multicolor visual detection of exosomes was realized by adjusting the aspect ratio of Au NBPs. It is worth mentioning that the Au NBP@MnO2 NSs was synthesized through in situ growth of MnO2 nanosheets on Au NBPs, and the attractive optical properties and ease of etching make Au NBP@MnO2 NSs promising candidates for plasmonic detection." @default.
• W3096807388 created "2020-11-09" @default.
• W3096807388 creator A5000900963 @default.
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• W3096807388 date "2020-10-27" @default.
• W3096807388 modified "2023-10-12" @default.
• W3096807388 title "Plasmonic Colorimetric Biosensor for Sensitive Exosome Detection via Enzyme-Induced Etching of Gold Nanobipyramid@MnO₂ Nanosheet Nanostructures" @default.
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• W3096807388 doi "https://doi.org/10.1021/acs.analchem.0c04136" @default.
• W3096807388 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33108733" @default.
• W3096807388 hasPublicationYear "2020" @default.
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