Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096912009> ?p ?o ?g. }
- W3096912009 endingPage "e0241287" @default.
- W3096912009 startingPage "e0241287" @default.
- W3096912009 abstract "Currently, cardiomyocyte (CM) differentiation methods require a purification step after CM induction to ensure the high purity of the cell population. Here we show an improved human CM differentiation protocol with which high-purity ventricular-type CMs can be obtained and maintained without any CM purification process. We induced and collected a mesodermal cell population (platelet-derived growth factor receptor-α (PDGFRα)-positive cells) that can respond to CM differentiation cues, and then stimulated CM differentiation by means of Wnt inhibition. This method reproducibly generated CMs with purities above 95% in several human pluripotent stem cell lines. Furthermore, these CM populations were maintained in culture at such high purity without any further CM purification step for over 200 days. The majority of these CMs (>95%) exhibited a ventricular-like phenotype with a tendency to structural and electrophysiological maturation, including T-tubule-like structure formation and the ability to respond to QT prolongation drugs. This is a simple and valuable method to stably generate CM populations suitable for cardiac toxicology testing, disease modeling and regenerative medicine." @default.
- W3096912009 created "2020-11-09" @default.
- W3096912009 creator A5014970655 @default.
- W3096912009 creator A5016134558 @default.
- W3096912009 creator A5019091228 @default.
- W3096912009 creator A5028592309 @default.
- W3096912009 creator A5068415395 @default.
- W3096912009 creator A5070981815 @default.
- W3096912009 creator A5081462823 @default.
- W3096912009 creator A5085652823 @default.
- W3096912009 creator A5091623593 @default.
- W3096912009 date "2020-11-02" @default.
- W3096912009 modified "2023-10-16" @default.
- W3096912009 title "Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells" @default.
- W3096912009 cites W1639628878 @default.
- W3096912009 cites W1869432913 @default.
- W3096912009 cites W1964521786 @default.
- W3096912009 cites W1970288681 @default.
- W3096912009 cites W1972530481 @default.
- W3096912009 cites W1978173381 @default.
- W3096912009 cites W1980831122 @default.
- W3096912009 cites W2010177021 @default.
- W3096912009 cites W2016157842 @default.
- W3096912009 cites W2021495513 @default.
- W3096912009 cites W2027433270 @default.
- W3096912009 cites W2033201483 @default.
- W3096912009 cites W2034862301 @default.
- W3096912009 cites W2035121417 @default.
- W3096912009 cites W2045626197 @default.
- W3096912009 cites W2048255936 @default.
- W3096912009 cites W2057257265 @default.
- W3096912009 cites W2059134277 @default.
- W3096912009 cites W2061132832 @default.
- W3096912009 cites W2061547804 @default.
- W3096912009 cites W2061725056 @default.
- W3096912009 cites W2062145052 @default.
- W3096912009 cites W2066390373 @default.
- W3096912009 cites W2070219190 @default.
- W3096912009 cites W2094280614 @default.
- W3096912009 cites W2099313405 @default.
- W3096912009 cites W2101889783 @default.
- W3096912009 cites W2101986300 @default.
- W3096912009 cites W2108892010 @default.
- W3096912009 cites W2109774918 @default.
- W3096912009 cites W2120328369 @default.
- W3096912009 cites W2122349854 @default.
- W3096912009 cites W2124929662 @default.
- W3096912009 cites W2128259747 @default.
- W3096912009 cites W2133127571 @default.
- W3096912009 cites W2141134447 @default.
- W3096912009 cites W2169859308 @default.
- W3096912009 cites W2191495149 @default.
- W3096912009 cites W2232640280 @default.
- W3096912009 cites W2236754528 @default.
- W3096912009 cites W2402146183 @default.
- W3096912009 cites W2559754868 @default.
- W3096912009 cites W2596682952 @default.
- W3096912009 cites W2742550673 @default.
- W3096912009 cites W2765838352 @default.
- W3096912009 cites W2774402858 @default.
- W3096912009 cites W2783837179 @default.
- W3096912009 cites W2792270966 @default.
- W3096912009 cites W2794616878 @default.
- W3096912009 cites W2898529438 @default.
- W3096912009 cites W3029730275 @default.
- W3096912009 cites W4211016865 @default.
- W3096912009 doi "https://doi.org/10.1371/journal.pone.0241287" @default.
- W3096912009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7605685" @default.
- W3096912009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33137106" @default.
- W3096912009 hasPublicationYear "2020" @default.
- W3096912009 type Work @default.
- W3096912009 sameAs 3096912009 @default.
- W3096912009 citedByCount "11" @default.
- W3096912009 countsByYear W30969120092021 @default.
- W3096912009 countsByYear W30969120092022 @default.
- W3096912009 countsByYear W30969120092023 @default.
- W3096912009 crossrefType "journal-article" @default.
- W3096912009 hasAuthorship W3096912009A5014970655 @default.
- W3096912009 hasAuthorship W3096912009A5016134558 @default.
- W3096912009 hasAuthorship W3096912009A5019091228 @default.
- W3096912009 hasAuthorship W3096912009A5028592309 @default.
- W3096912009 hasAuthorship W3096912009A5068415395 @default.
- W3096912009 hasAuthorship W3096912009A5070981815 @default.
- W3096912009 hasAuthorship W3096912009A5081462823 @default.
- W3096912009 hasAuthorship W3096912009A5085652823 @default.
- W3096912009 hasAuthorship W3096912009A5091623593 @default.
- W3096912009 hasBestOaLocation W30969120091 @default.
- W3096912009 hasConcept C104317684 @default.
- W3096912009 hasConcept C107459253 @default.
- W3096912009 hasConcept C10854531 @default.
- W3096912009 hasConcept C137620995 @default.
- W3096912009 hasConcept C145103041 @default.
- W3096912009 hasConcept C148738053 @default.
- W3096912009 hasConcept C1491633281 @default.
- W3096912009 hasConcept C28328180 @default.
- W3096912009 hasConcept C2908647359 @default.
- W3096912009 hasConcept C54355233 @default.
- W3096912009 hasConcept C55493867 @default.