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• W3096930621 abstract "Background: Extracellular ATP is the powerful trigger of neuroinflammation mainly via activation of pro-inflammatory P2X7 receptors. Acetylcholine and nicotinic agonists can inhibit ATP-triggered release of the pro-inflammatory cytokines from immune cells via so-called ‘Cholinergic Anti-Inflammatory Pathway’. However, it remains unclear at what stage of ATP-induced signaling cholinergic agents provide this anti-inflammatory effect. Using the specific property of P2X7 receptor to open a pathway permeable to large molecules, associated with activation of inflammasome, we studied the action of cholinergic agents on the initial steps of P2X7 receptor activation. Methods: Freshly isolated mouse peritoneal mast cells, used as model of immune cells, were treated with ATP alone or in the presence of acetylcholine and nicotine. To assess P2X7 channels opening, mast cells permeability to the fluorescent dye YO-PRO1 was measured by the flow cytometry approach. Results: ATP efficiently opened P2X7 ion channels in mast cells, shown by the large uptake of YO-PRO1. This stimulatory effect was inhibited by the specific P2X7 antagonist A839977 confirming that YO-PRO1 uptake was mediated via ATP-gated P2X7 ion channels. Interestingly, cholinergic agents also slightly enhanced permeability of mast cells to YO-PRO1. However, both acetylcholine and nicotine failed to inhibit the stimulatory effect of ATP on P2X7 receptors. Conclusion. These data suggest that cholinergic agents do not act on the initial steps of P2X7 receptor activation, but likely mediate the anti-inflammatory effect downstream of ATP-driven signaling, probably at the stage of processing or release of the pro-inflammatory cytokines." @default.
• W3096930621 created "2020-11-09" @default.
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• W3096930621 date "2020-10-28" @default.
• W3096930621 modified "2023-09-23" @default.
• W3096930621 title "Does Cholinergic Stimulation Affect the P2X7 Receptor-Mediated Dye Uptake in Mast Cells and Macrophages?" @default.
• W3096930621 cites W1532275077 @default.
• W3096930621 cites W1605844554 @default.
• W3096930621 cites W1824528611 @default.
• W3096930621 cites W1925634785 @default.
• W3096930621 cites W1975030382 @default.
• W3096930621 cites W1979278210 @default.
• W3096930621 cites W1982386486 @default.
• W3096930621 cites W1985620187 @default.
• W3096930621 cites W1990063735 @default.
• W3096930621 cites W2003764481 @default.
• W3096930621 cites W2009523990 @default.
• W3096930621 cites W2009614275 @default.
• W3096930621 cites W2015163544 @default.
• W3096930621 cites W2018424479 @default.
• W3096930621 cites W2019057590 @default.
• W3096930621 cites W2025634320 @default.
• W3096930621 cites W2029403824 @default.
• W3096930621 cites W2040479226 @default.
• W3096930621 cites W2046318104 @default.
• W3096930621 cites W2054921672 @default.
• W3096930621 cites W2066784771 @default.
• W3096930621 cites W2069368135 @default.
• W3096930621 cites W2073295417 @default.
• W3096930621 cites W2078156178 @default.
• W3096930621 cites W2079882002 @default.
• W3096930621 cites W2083906701 @default.
• W3096930621 cites W2084383502 @default.
• W3096930621 cites W2089597910 @default.
• W3096930621 cites W2100410594 @default.
• W3096930621 cites W2102694854 @default.
• W3096930621 cites W2110477569 @default.
• W3096930621 cites W2131942512 @default.
• W3096930621 cites W2132430680 @default.
• W3096930621 cites W2151579964 @default.
• W3096930621 cites W2154040034 @default.
• W3096930621 cites W2155373881 @default.
• W3096930621 cites W2156346381 @default.
• W3096930621 cites W2159748862 @default.
• W3096930621 cites W2164688291 @default.
• W3096930621 cites W2172277776 @default.
• W3096930621 cites W2281069839 @default.
• W3096930621 cites W2292051166 @default.
• W3096930621 cites W2300256671 @default.
• W3096930621 cites W2396424530 @default.
• W3096930621 cites W2410033682 @default.
• W3096930621 cites W2468706945 @default.
• W3096930621 cites W2470531925 @default.
• W3096930621 cites W2551737554 @default.
• W3096930621 cites W2560668196 @default.
• W3096930621 cites W2561139965 @default.
• W3096930621 cites W2566463713 @default.
• W3096930621 cites W2594371417 @default.
• W3096930621 cites W2728782542 @default.
• W3096930621 cites W2737555531 @default.
• W3096930621 cites W2749454667 @default.
• W3096930621 cites W2753123378 @default.
• W3096930621 cites W2755544565 @default.
• W3096930621 cites W2759874929 @default.
• W3096930621 cites W2772437893 @default.
• W3096930621 cites W2792040292 @default.
• W3096930621 cites W2793479725 @default.
• W3096930621 cites W2794673243 @default.
• W3096930621 cites W2797467000 @default.
• W3096930621 cites W2801296114 @default.
• W3096930621 cites W2808479626 @default.
• W3096930621 cites W2886676544 @default.
• W3096930621 cites W2900726683 @default.
• W3096930621 cites W2911889580 @default.
• W3096930621 cites W2912358317 @default.
• W3096930621 cites W2943009190 @default.
• W3096930621 cites W2943942743 @default.
• W3096930621 cites W2969921857 @default.
• W3096930621 cites W2978654870 @default.
• W3096930621 cites W2993948719 @default.
• W3096930621 cites W3007908772 @default.
• W3096930621 cites W3010697254 @default.
• W3096930621 cites W3011061263 @default.
• W3096930621 cites W3013043209 @default.
• W3096930621 cites W3015471235 @default.
• W3096930621 cites W3026978470 @default.
• W3096930621 doi "https://doi.org/10.3389/fncel.2020.548376" @default.
• W3096930621 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7673375" @default.
• W3096930621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33328886" @default.
• W3096930621 hasPublicationYear "2020" @default.
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