FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3096938332> ?p ?o ?g. }

• W3096938332 abstract "Background: Candida auris is an emerging pathogen that has recently disseminated globally and caused challenging outbreaks in healthcare facilities (HCFs), in part because it is commonly multidrug-resistant. Candida auris remains rare in Canada, with ~20 known cases to date. We describe the emergence of a novel subclade of C. auris in Ontario, Canada, using whole-genome sequencing (WGS). Methods: In Ontario, many HCFs submit yeast isolates from sterile sites requiring species-level characterization and antifungal susceptibility testing (AFST) to the provincial reference laboratory. Yeasts were identified using a combination of standard methods (morphology, API 20C, MALDI-ToF MS) including ITS2 sequencing. Sensititre YO9 panels were used for AFST. Genomic analysis of C. auris was performed using an Illumina HiSeq platform with at least 50× coverage; variants were called against the reference genome by using the previously published North Arizona SNP pipeline (NASP). Phylogenetic trees were produced by maximum parsimony method (MEGA7.0). Results: Between 2014 and 2018, yeast isolates from 5 different patients from 4 HCFs in the same region of Ontario were confirmed to be C. auris by ITS2 PCR and sequence analysis (Table 1). Based on interim CDC criteria for antifungal drug break points, all isolates were pansusceptible to common antifungals. WGS analysis demonstrated that the C. auris isolates were part of the South American clade (IV) and formed an isolated subclade that is well supported by bootstrap analysis, indicating clonal relationships among these isolates (Fig. 1). Conclusions: Although C. auris isolates are usually drug resistant, all 5 initial Ontario isolates were pansusceptible. WGS determined that these isolates clustered within clade IV and were clonal. This cluster of C. auris appears to represent a new subclade of the South American clade that has been transmitted among patients within a region of Ontario. C. auris may have been present in Ontario for some time, escaping earlier detection due to lack of screening programs in HCFs, historical challenges with microbiologic detection of C. auris , and the antifungal susceptibility of the circulating isolates. Investigations are underway to determine clinical features and epidemiologic relatedness among patients in this cluster. Funding: None Disclosures: Susy Hota, Contracted Research - Finch Therapeutics" @default.
• W3096938332 created "2020-11-09" @default.
• W3096938332 creator A5001970218 @default.
• W3096938332 creator A5002931774 @default.
• W3096938332 creator A5020092003 @default.
• W3096938332 creator A5036896044 @default.
• W3096938332 creator A5038131221 @default.
• W3096938332 creator A5042035828 @default.
• W3096938332 creator A5044834575 @default.
• W3096938332 creator A5045779775 @default.
• W3096938332 creator A5048365064 @default.
• W3096938332 creator A5055080379 @default.
• W3096938332 creator A5074720003 @default.
• W3096938332 creator A5077960205 @default.
• W3096938332 creator A5078880136 @default.
• W3096938332 creator A5089720853 @default.
• W3096938332 date "2020-10-01" @default.
• W3096938332 modified "2023-09-25" @default.
• W3096938332 title "Whole-Genome Sequencing Reveals a Novel Subclade of Pansusceptible Candida auris in Ontario, Canada" @default.
• W3096938332 doi "https://doi.org/10.1017/ice.2020.542" @default.
• W3096938332 hasPublicationYear "2020" @default.
• W3096938332 type Work @default.
• W3096938332 sameAs 3096938332 @default.
• W3096938332 citedByCount "0" @default.
• W3096938332 crossrefType "journal-article" @default.
• W3096938332 hasAuthorship W3096938332A5001970218 @default.
• W3096938332 hasAuthorship W3096938332A5002931774 @default.
• W3096938332 hasAuthorship W3096938332A5020092003 @default.
• W3096938332 hasAuthorship W3096938332A5036896044 @default.
• W3096938332 hasAuthorship W3096938332A5038131221 @default.
• W3096938332 hasAuthorship W3096938332A5042035828 @default.
• W3096938332 hasAuthorship W3096938332A5044834575 @default.
• W3096938332 hasAuthorship W3096938332A5045779775 @default.
• W3096938332 hasAuthorship W3096938332A5048365064 @default.
• W3096938332 hasAuthorship W3096938332A5055080379 @default.
• W3096938332 hasAuthorship W3096938332A5074720003 @default.
• W3096938332 hasAuthorship W3096938332A5077960205 @default.
• W3096938332 hasAuthorship W3096938332A5078880136 @default.
• W3096938332 hasAuthorship W3096938332A5089720853 @default.
• W3096938332 hasBestOaLocation W30969383321 @default.
• W3096938332 hasConcept C104317684 @default.
• W3096938332 hasConcept C141231307 @default.
• W3096938332 hasConcept C193252679 @default.
• W3096938332 hasConcept C200197925 @default.
• W3096938332 hasConcept C24432333 @default.
• W3096938332 hasConcept C2779548794 @default.
• W3096938332 hasConcept C2909786013 @default.
• W3096938332 hasConcept C44465124 @default.
• W3096938332 hasConcept C54355233 @default.
• W3096938332 hasConcept C86803240 @default.
• W3096938332 hasConcept C89423630 @default.
• W3096938332 hasConceptScore W3096938332C104317684 @default.
• W3096938332 hasConceptScore W3096938332C141231307 @default.
• W3096938332 hasConceptScore W3096938332C193252679 @default.
• W3096938332 hasConceptScore W3096938332C200197925 @default.
• W3096938332 hasConceptScore W3096938332C24432333 @default.
• W3096938332 hasConceptScore W3096938332C2779548794 @default.
• W3096938332 hasConceptScore W3096938332C2909786013 @default.
• W3096938332 hasConceptScore W3096938332C44465124 @default.
• W3096938332 hasConceptScore W3096938332C54355233 @default.
• W3096938332 hasConceptScore W3096938332C86803240 @default.
• W3096938332 hasConceptScore W3096938332C89423630 @default.
• W3096938332 hasLocation W30969383321 @default.
• W3096938332 hasOpenAccess W3096938332 @default.
• W3096938332 hasPrimaryLocation W30969383321 @default.
• W3096938332 hasRelatedWork W1794179 @default.
• W3096938332 hasRelatedWork W21265334 @default.
• W3096938332 hasRelatedWork W24052344 @default.
• W3096938332 hasRelatedWork W24121602 @default.
• W3096938332 hasRelatedWork W24590886 @default.
• W3096938332 hasRelatedWork W26537840 @default.
• W3096938332 hasRelatedWork W35481901 @default.
• W3096938332 hasRelatedWork W39608448 @default.
• W3096938332 hasRelatedWork W7060423 @default.
• W3096938332 hasRelatedWork W17583157 @default.
• W3096938332 isParatext "false" @default.
• W3096938332 isRetracted "false" @default.
• W3096938332 magId "3096938332" @default.
• W3096938332 workType "article" @default.