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• W3097320121 endingPage "9385" @default.
• W3097320121 startingPage "9372" @default.
• W3097320121 abstract "Efficient neurotransmitter release at the presynaptic terminal requires docking of synaptic vesicles to the active zone membrane and formation of fusion-competent synaptic vesicles near voltage-gated Ca 2+ channels. Rab3-interacting molecule (RIM) is a critical active zone organizer, as it recruits Ca 2+ channels and activates synaptic vesicle docking and priming via Munc13-1. However, our knowledge about Munc13-independent contributions of RIM to active zone functions is limited. To identify the functions that are solely mediated by RIM, we used genetic manipulations to control RIM and Munc13-1 activity in cultured hippocampal neurons from mice of either sex and compared synaptic ultrastructure and neurotransmission. We found that RIM modulates synaptic vesicle localization in the proximity of the active zone membrane independent of Munc13-1. In another step, both RIM and Munc13 mediate synaptic vesicle docking and priming. In addition, while the activity of both RIM and Munc13-1 is required for Ca 2+ -evoked release, RIM uniquely controls neurotransmitter release efficiency. However, activity-dependent augmentation of synaptic vesicle pool size relies exclusively on the action of Munc13s. Based on our results, we extend previous findings and propose a refined model in which RIM and Munc13-1 act in overlapping and independent stages of synaptic vesicle localization and release. SIGNIFICANCE STATEMENT The presynaptic active zone is composed of scaffolding proteins that functionally interact to localize synaptic vesicles to release sites, ensuring neurotransmission. Our current knowledge of the presynaptic active zone function relies on structure-function analysis, which has provided detailed information on the network of interactions and the impact of active zone proteins. Yet, the hierarchical, redundant, or independent cooperation of each active zone protein to synapse functions is not fully understood. Rab3-interacting molecule and Munc13 are the two key functionally interacting active zone proteins. Here, we dissected the distinct actions of Rab3-interacting molecule and Munc13-1 from both ultrastructural and physiological aspects. Our findings provide a more detailed view of how these two presynaptic proteins orchestrate their functions to achieve synaptic transmission." @default.
• W3097320121 created "2020-11-09" @default.
• W3097320121 creator A5001571055 @default.
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• W3097320121 date "2020-11-02" @default.
• W3097320121 modified "2023-10-14" @default.
• W3097320121 title "Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission" @default.
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• W3097320121 doi "https://doi.org/10.1523/jneurosci.1922-20.2020" @default.
• W3097320121 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7724145" @default.
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