Matches in SemOpenAlex for { <https://semopenalex.org/work/W3097369531> ?p ?o ?g. }
- W3097369531 abstract "Abstract The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin." @default.
- W3097369531 created "2020-11-09" @default.
- W3097369531 creator A5000734401 @default.
- W3097369531 creator A5005928603 @default.
- W3097369531 creator A5024519448 @default.
- W3097369531 creator A5029195076 @default.
- W3097369531 creator A5032615960 @default.
- W3097369531 creator A5039180019 @default.
- W3097369531 creator A5043239620 @default.
- W3097369531 creator A5050285122 @default.
- W3097369531 creator A5063523432 @default.
- W3097369531 creator A5067839520 @default.
- W3097369531 creator A5070964696 @default.
- W3097369531 creator A5074393963 @default.
- W3097369531 date "2020-11-04" @default.
- W3097369531 modified "2023-10-16" @default.
- W3097369531 title "Evolution from adherent to suspension: systems biology of HEK293 cell line development" @default.
- W3097369531 cites W1662925407 @default.
- W3097369531 cites W1818313583 @default.
- W3097369531 cites W1915519529 @default.
- W3097369531 cites W1970541648 @default.
- W3097369531 cites W1972978214 @default.
- W3097369531 cites W1974307799 @default.
- W3097369531 cites W1980863165 @default.
- W3097369531 cites W1985000845 @default.
- W3097369531 cites W1986406786 @default.
- W3097369531 cites W1994114235 @default.
- W3097369531 cites W1995046405 @default.
- W3097369531 cites W1998460146 @default.
- W3097369531 cites W2008071343 @default.
- W3097369531 cites W2017514564 @default.
- W3097369531 cites W2021811013 @default.
- W3097369531 cites W2023580865 @default.
- W3097369531 cites W2030196316 @default.
- W3097369531 cites W2030728180 @default.
- W3097369531 cites W2036411040 @default.
- W3097369531 cites W2042340758 @default.
- W3097369531 cites W2044296969 @default.
- W3097369531 cites W2047899060 @default.
- W3097369531 cites W2049762689 @default.
- W3097369531 cites W2049974202 @default.
- W3097369531 cites W2052407981 @default.
- W3097369531 cites W2061604182 @default.
- W3097369531 cites W2062537218 @default.
- W3097369531 cites W2062989416 @default.
- W3097369531 cites W2063011414 @default.
- W3097369531 cites W2067094344 @default.
- W3097369531 cites W2068211237 @default.
- W3097369531 cites W2069053195 @default.
- W3097369531 cites W2071888724 @default.
- W3097369531 cites W2072880634 @default.
- W3097369531 cites W2073824045 @default.
- W3097369531 cites W2081306620 @default.
- W3097369531 cites W2088484810 @default.
- W3097369531 cites W2093343938 @default.
- W3097369531 cites W2093362562 @default.
- W3097369531 cites W2094068725 @default.
- W3097369531 cites W2094327712 @default.
- W3097369531 cites W2099350955 @default.
- W3097369531 cites W2102619694 @default.
- W3097369531 cites W2103441770 @default.
- W3097369531 cites W2106136854 @default.
- W3097369531 cites W2106578986 @default.
- W3097369531 cites W2108234281 @default.
- W3097369531 cites W2109241881 @default.
- W3097369531 cites W2111281047 @default.
- W3097369531 cites W2116710527 @default.
- W3097369531 cites W2116764617 @default.
- W3097369531 cites W2117045376 @default.
- W3097369531 cites W2119180969 @default.
- W3097369531 cites W2124653577 @default.
- W3097369531 cites W2134672574 @default.
- W3097369531 cites W2135643214 @default.
- W3097369531 cites W2147672037 @default.
- W3097369531 cites W2148351505 @default.
- W3097369531 cites W2160509178 @default.
- W3097369531 cites W2161031052 @default.
- W3097369531 cites W2164740045 @default.
- W3097369531 cites W2165918134 @default.
- W3097369531 cites W2167778120 @default.
- W3097369531 cites W2168085358 @default.
- W3097369531 cites W2168133698 @default.
- W3097369531 cites W2179438025 @default.
- W3097369531 cites W2197124664 @default.
- W3097369531 cites W2277906934 @default.
- W3097369531 cites W2303656007 @default.
- W3097369531 cites W2323326409 @default.
- W3097369531 cites W2337209831 @default.
- W3097369531 cites W2341539131 @default.
- W3097369531 cites W2345356016 @default.
- W3097369531 cites W2492699395 @default.
- W3097369531 cites W2585978400 @default.
- W3097369531 cites W2608656888 @default.
- W3097369531 cites W2615010141 @default.
- W3097369531 cites W2618796452 @default.
- W3097369531 cites W2748878638 @default.
- W3097369531 cites W2749613778 @default.
- W3097369531 cites W2754820805 @default.
- W3097369531 cites W2917101543 @default.
- W3097369531 cites W2917973694 @default.