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- W3098063634 abstract "Abstract Bacteria often employ different respiratory chains that comprise membrane proteins equipped with various cofactors. Monitoring the protein inventory that is present in the cells under a given cultivation condition is often difficult and time‐consuming. One example of a metabolically versatile bacterium is the microaerophilic organohalide‐respiring Sulfurospirillum multivorans . Here, we used surface enhanced Raman spectroscopy (SERS) to quickly identify the cofactors involved in the respiration of S. multivorans . We cultured the organism with either tetrachloroethene (perchloroethylene, PCE), fumarate, nitrate, or oxygen as electron acceptors. Because the corresponding terminal reductases of the four different respiratory chains harbor different cofactors, specific fingerprint signals in SERS were expected. Silver nanostructures fabricated by means of electron beam lithography were coated with the membrane fractions extracted from the four S. multivorans cultivations, and SERS spectra were recorded. In the case of S. multivorans cultivated with PCE, the recorded SERS spectra were dominated by Raman peaks specific for Vitamin B 12 . This is attributed to the high abundance of the PCE reductive dehalogenase (PceA), the key enzyme in PCE respiration. After cultivation with oxygen, fumarate, or nitrate, no Raman spectral features of B 12 were found." @default.
- W3098063634 created "2020-11-23" @default.
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- W3098063634 date "2020-11-18" @default.
- W3098063634 modified "2023-10-17" @default.
- W3098063634 title "Surface enhanced Raman spectroscopy‐based evaluation of the membrane protein composition of the organohalide‐respiring <i>Sulfurospirillum multivorans</i>" @default.
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- W3098063634 doi "https://doi.org/10.1002/jrs.6029" @default.
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