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- W3098084501 abstract "Surface-enhanced Raman spectroscopy (SERS) sensing of DNA sequences by plasmonic nanopores could pave a way to new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. However, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. In this work, we report an approach to control the time that molecules reside in the hot spot by physically adsorbing them onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole. By trapping the nanoparticle for up to minutes, we demonstrate single-molecule SERS detection of all 4 DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins." @default.
- W3098084501 created "2020-11-23" @default.
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- W3098084501 date "2019-11-22" @default.
- W3098084501 modified "2023-10-12" @default.
- W3098084501 title "SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping" @default.
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- W3098084501 doi "https://doi.org/10.1038/s41467-019-13242-x" @default.
- W3098084501 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6874578" @default.
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