Matches in SemOpenAlex for { <https://semopenalex.org/work/W3098264474> ?p ?o ?g. }
- W3098264474 abstract "Abstract AAA+ ring-shaped machines, such as ClpB and Hsp104, mediate substrate translocation through their central channel by a set of pore loops. Recent structural studies suggested a universal hand-over-hand translocation mechanism, in which pore loops are moving rigidly in tandem with their corresponding subunits. However, functional and biophysical studies are in discord with this model. Here, we directly measure the real-time dynamics of the pore loops of ClpB and their response to substrate binding, using single-molecule FRET spectroscopy. All pore loops undergo large-amplitude fluctuations on the microsecond timescale, and change their conformation upon interaction with substrate proteins. Pore-loop conformational dynamics are modulated by nucleotides and strongly correlate with disaggregation activity. The differential behavior of the pore loops along the axial channel points to a fast Brownian-ratchet translocation mechanism, which likely acts in parallel to the much slower hand-over-hand process." @default.
- W3098264474 created "2020-11-23" @default.
- W3098264474 creator A5003319572 @default.
- W3098264474 creator A5034896718 @default.
- W3098264474 creator A5036157581 @default.
- W3098264474 creator A5089385932 @default.
- W3098264474 date "2020-11-19" @default.
- W3098264474 modified "2023-09-25" @default.
- W3098264474 title "Ultrafast Brownian-ratchet mechanism for protein translocation by a AAA+ machine" @default.
- W3098264474 cites W1601050661 @default.
- W3098264474 cites W1857769113 @default.
- W3098264474 cites W2007590689 @default.
- W3098264474 cites W2010958487 @default.
- W3098264474 cites W2037319865 @default.
- W3098264474 cites W2046105451 @default.
- W3098264474 cites W2059029715 @default.
- W3098264474 cites W2059265460 @default.
- W3098264474 cites W2062657409 @default.
- W3098264474 cites W2072475098 @default.
- W3098264474 cites W2074654002 @default.
- W3098264474 cites W2088313477 @default.
- W3098264474 cites W2130119946 @default.
- W3098264474 cites W2193963378 @default.
- W3098264474 cites W2346906930 @default.
- W3098264474 cites W2526551102 @default.
- W3098264474 cites W2557417208 @default.
- W3098264474 cites W2625636492 @default.
- W3098264474 cites W2744996671 @default.
- W3098264474 cites W2755451787 @default.
- W3098264474 cites W2768657824 @default.
- W3098264474 cites W2782758955 @default.
- W3098264474 cites W2886922109 @default.
- W3098264474 cites W2893067023 @default.
- W3098264474 cites W2913971431 @default.
- W3098264474 cites W2923826920 @default.
- W3098264474 cites W2969224557 @default.
- W3098264474 cites W2972322850 @default.
- W3098264474 cites W2989746390 @default.
- W3098264474 cites W3003864032 @default.
- W3098264474 cites W3007966208 @default.
- W3098264474 cites W3030138469 @default.
- W3098264474 cites W3090529796 @default.
- W3098264474 cites W4236249766 @default.
- W3098264474 doi "https://doi.org/10.1101/2020.11.19.384313" @default.
- W3098264474 hasPublicationYear "2020" @default.
- W3098264474 type Work @default.
- W3098264474 sameAs 3098264474 @default.
- W3098264474 citedByCount "2" @default.
- W3098264474 countsByYear W30982644742021 @default.
- W3098264474 crossrefType "posted-content" @default.
- W3098264474 hasAuthorship W3098264474A5003319572 @default.
- W3098264474 hasAuthorship W3098264474A5034896718 @default.
- W3098264474 hasAuthorship W3098264474A5036157581 @default.
- W3098264474 hasAuthorship W3098264474A5089385932 @default.
- W3098264474 hasBestOaLocation W30982644741 @default.
- W3098264474 hasConcept C112401455 @default.
- W3098264474 hasConcept C121332964 @default.
- W3098264474 hasConcept C121864883 @default.
- W3098264474 hasConcept C12554922 @default.
- W3098264474 hasConcept C130603088 @default.
- W3098264474 hasConcept C159467904 @default.
- W3098264474 hasConcept C185592680 @default.
- W3098264474 hasConcept C18762648 @default.
- W3098264474 hasConcept C18903297 @default.
- W3098264474 hasConcept C2777289219 @default.
- W3098264474 hasConcept C2777694918 @default.
- W3098264474 hasConcept C2780004032 @default.
- W3098264474 hasConcept C62520636 @default.
- W3098264474 hasConcept C8010536 @default.
- W3098264474 hasConcept C86803240 @default.
- W3098264474 hasConcept C91881484 @default.
- W3098264474 hasConcept C96305047 @default.
- W3098264474 hasConcept C97355855 @default.
- W3098264474 hasConceptScore W3098264474C112401455 @default.
- W3098264474 hasConceptScore W3098264474C121332964 @default.
- W3098264474 hasConceptScore W3098264474C121864883 @default.
- W3098264474 hasConceptScore W3098264474C12554922 @default.
- W3098264474 hasConceptScore W3098264474C130603088 @default.
- W3098264474 hasConceptScore W3098264474C159467904 @default.
- W3098264474 hasConceptScore W3098264474C185592680 @default.
- W3098264474 hasConceptScore W3098264474C18762648 @default.
- W3098264474 hasConceptScore W3098264474C18903297 @default.
- W3098264474 hasConceptScore W3098264474C2777289219 @default.
- W3098264474 hasConceptScore W3098264474C2777694918 @default.
- W3098264474 hasConceptScore W3098264474C2780004032 @default.
- W3098264474 hasConceptScore W3098264474C62520636 @default.
- W3098264474 hasConceptScore W3098264474C8010536 @default.
- W3098264474 hasConceptScore W3098264474C86803240 @default.
- W3098264474 hasConceptScore W3098264474C91881484 @default.
- W3098264474 hasConceptScore W3098264474C96305047 @default.
- W3098264474 hasConceptScore W3098264474C97355855 @default.
- W3098264474 hasLocation W30982644741 @default.
- W3098264474 hasOpenAccess W3098264474 @default.
- W3098264474 hasPrimaryLocation W30982644741 @default.
- W3098264474 hasRelatedWork W2011881742 @default.
- W3098264474 hasRelatedWork W2101834254 @default.
- W3098264474 hasRelatedWork W2359161904 @default.
- W3098264474 hasRelatedWork W2770608935 @default.
- W3098264474 hasRelatedWork W3024672144 @default.
- W3098264474 hasRelatedWork W3103070464 @default.