FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3098595242> ?p ?o ?g. }

• W3098595242 abstract "Abstract Defective endocytosis and vesicular trafficking of signaling receptors has recently emerged as a multifaceted hallmark of malignant cells. Clathrin-coated pits (CCPs), the fundamental unit of clathrin-mediated endocytosis, display highly heterogeneous dynamics on the plasma membrane where they can take from 20 seconds to over a minute to form cytosolic coated-vesicles. Despite the large number of cargo molecules that traffic through CCPs, it is not well understood whether signaling receptors activated in cancer, such as epidermal growth factor receptor (EGFR), are regulated through a specific subset of CCPs. The signaling lipid phosphatidylinositol (3,4,5)-triphosphate (PI(3,4,5)P 3 ), which is dephosphorylated by phosphatase tensin homolog (PTEN), is a potent tumorigenic signaling lipid that is present in excess in many types of cancers. Using total internal reflection fluorescence microscopy and automated tracking and detection of CCPs, we find PTEN and EGF bound EGFR are enriched in a distinct subset of short-lived CCPs that corresponded with clathrin-dependent EGF-induced signaling. By deleting PTEN using CRISPR-Cas9 and reconstituting PTEN, we demonstrate that PTEN plays a role in the regulation of CCP dynamics; this appears to recapitulate CCP dynamics in highly metastatic PTEN-deleted cancer cells where we find a larger proportion of short-lived CCPs and higher initiation density compared to the normal cells. Furthermore, increased PI(3,4,5)P 3 results in higher proportion of short-lived CCPs, an effect that recapitulates PTEN deletion. Our findings provide strong evidence for the existence of short-lived ‘signaling-capable’ CCPs. Altogether, these findings demonstrate the importance of PTEN and PI(3,4,5)P 3 in regulating CCP dynamics and assign a new function to PTEN as a modulator of signaling-capable CCPs." @default.
• W3098595242 created "2020-11-23" @default.
• W3098595242 creator A5001179154 @default.
• W3098595242 creator A5001811088 @default.
• W3098595242 creator A5005497098 @default.
• W3098595242 creator A5008651883 @default.
• W3098595242 creator A5025643197 @default.
• W3098595242 creator A5027096080 @default.
• W3098595242 creator A5028518958 @default.
• W3098595242 creator A5037238891 @default.
• W3098595242 creator A5042975888 @default.
• W3098595242 creator A5054339536 @default.
• W3098595242 creator A5055543052 @default.
• W3098595242 creator A5058502408 @default.
• W3098595242 creator A5058661506 @default.
• W3098595242 creator A5067577744 @default.
• W3098595242 creator A5071608210 @default.
• W3098595242 date "2017-05-14" @default.
• W3098595242 modified "2023-10-03" @default.
• W3098595242 title "Loss of PTEN promotes formation of signaling-capable clathrin-coated pits" @default.
• W3098595242 cites W1914617157 @default.
• W3098595242 cites W1935374681 @default.
• W3098595242 cites W1971060242 @default.
• W3098595242 cites W1971746394 @default.
• W3098595242 cites W1989482277 @default.
• W3098595242 cites W1998038367 @default.
• W3098595242 cites W1998140525 @default.
• W3098595242 cites W1998263911 @default.
• W3098595242 cites W1998622911 @default.
• W3098595242 cites W2002516761 @default.
• W3098595242 cites W2011625746 @default.
• W3098595242 cites W2015562061 @default.
• W3098595242 cites W2019196882 @default.
• W3098595242 cites W2019772485 @default.
• W3098595242 cites W2026626098 @default.
• W3098595242 cites W2027639590 @default.
• W3098595242 cites W2029943273 @default.
• W3098595242 cites W2033344378 @default.
• W3098595242 cites W2040010587 @default.
• W3098595242 cites W2045880404 @default.
• W3098595242 cites W2046200821 @default.
• W3098595242 cites W2049253479 @default.
• W3098595242 cites W2071716012 @default.
• W3098595242 cites W2073576660 @default.
• W3098595242 cites W2074325696 @default.
• W3098595242 cites W2075827435 @default.
• W3098595242 cites W2094882170 @default.
• W3098595242 cites W2096154351 @default.
• W3098595242 cites W2097721584 @default.
• W3098595242 cites W2101189847 @default.
• W3098595242 cites W2102580914 @default.
• W3098595242 cites W2103642422 @default.
• W3098595242 cites W2105916176 @default.
• W3098595242 cites W2115994196 @default.
• W3098595242 cites W2119809698 @default.
• W3098595242 cites W2124335390 @default.
• W3098595242 cites W2125037432 @default.
• W3098595242 cites W2125319008 @default.
• W3098595242 cites W2128491331 @default.
• W3098595242 cites W2129546321 @default.
• W3098595242 cites W2133315130 @default.
• W3098595242 cites W2135721953 @default.
• W3098595242 cites W2135931614 @default.
• W3098595242 cites W2141466024 @default.
• W3098595242 cites W2143898087 @default.
• W3098595242 cites W2152960244 @default.
• W3098595242 cites W2152988906 @default.
• W3098595242 cites W2153121397 @default.
• W3098595242 cites W2154156417 @default.
• W3098595242 cites W2156918228 @default.
• W3098595242 cites W2157643349 @default.
• W3098595242 cites W2165130769 @default.
• W3098595242 cites W2167806590 @default.
• W3098595242 cites W2170296344 @default.
• W3098595242 cites W2179272349 @default.
• W3098595242 cites W2191370645 @default.
• W3098595242 cites W2253412135 @default.
• W3098595242 cites W2282348015 @default.
• W3098595242 cites W2298821037 @default.
• W3098595242 cites W2310769659 @default.
• W3098595242 cites W2480355574 @default.
• W3098595242 cites W2493148378 @default.
• W3098595242 doi "https://doi.org/10.1101/137760" @default.
• W3098595242 hasPublicationYear "2017" @default.
• W3098595242 type Work @default.
• W3098595242 sameAs 3098595242 @default.
• W3098595242 citedByCount "1" @default.
• W3098595242 countsByYear W30985952422020 @default.
• W3098595242 crossrefType "posted-content" @default.
• W3098595242 hasAuthorship W3098595242A5001179154 @default.
• W3098595242 hasAuthorship W3098595242A5001811088 @default.
• W3098595242 hasAuthorship W3098595242A5005497098 @default.
• W3098595242 hasAuthorship W3098595242A5008651883 @default.
• W3098595242 hasAuthorship W3098595242A5025643197 @default.
• W3098595242 hasAuthorship W3098595242A5027096080 @default.
• W3098595242 hasAuthorship W3098595242A5028518958 @default.
• W3098595242 hasAuthorship W3098595242A5037238891 @default.
• W3098595242 hasAuthorship W3098595242A5042975888 @default.
• W3098595242 hasAuthorship W3098595242A5054339536 @default.
• W3098595242 hasAuthorship W3098595242A5055543052 @default.

• next