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• W3098796618 abstract "The pressure-jump relaxation technique is a convenient and interesting means of studying rapid reversible reactions of biological systems. According to the change in reaction volume that accompanies a biochemical process, a rapid pressure change Δp produces a relative equilibrium shift ΔK/K, which is given by ΔlnK = AK/K = — (ΔV°/RT) Δp, where ΔV° is the reaction volume change. If the pressure change has a very short transition time, then relaxation kinetic measurements near equilibrium are possible, allowing the elucidation of reaction mechanisms through the detection of eventual reaction intermediates and the characterization of elementary kinetic and thermodynamic parameters. Our reversible pressure-jump method described in this chapter is capable of producing a sharp pressure change of ±20MPa in less than 3 milliseconds allowing the determination of relaxation rates in the time range of several milliseconds to several minutes at any final pressure up to 400 MPa, and in any viscosity solution. This technique was employed to study the binding kinetics of camphor and its analogues to bacterial cytochrome P-450cam as functions of temperature, pressure, and viscosity. The results obtained are discussed in terms of conformational dynamics of the protein associated with the entry and the exist of water molecules and specific interactions of the substrate1 with the apolar residues in the active site of cytochrome P-450cam. The binding of ligands or substrates to proteins can exhibit multistate kinetic behavior similar to transient-stale enzyme kinetics and isomerizations of proteins. The underlying elementary reaction mechanisms can be elucidated by the use of rapid mixing techniques. Usually a reaction is initiated by mixing the reactants as rapidly as possible, and the approach to equilibrium is monitored. This method has been adapted to the study of enzyme reaction mechanisms under extreme conditions of temperature and pressure (Hui Bon Hoa & Douzou, 1973; Balny et al., 1984). However, this approach is limited by the deadtime, the large amount of sample required, and difficulties in using the apparatus to study viscous solutions, such as Schlieren effects, caused by incomplete mixing in flow experiments. Relaxation techniques overcome these problems by the application of a physical perturbation to a system already at equilibrium." @default.
• W3098796618 created "2020-11-23" @default.
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• W3098796618 date "1996-10-10" @default.
• W3098796618 modified "2023-09-26" @default.
• W3098796618 title "Application of Pressure Relaxation to the Study of Substrate Binding to Cytochrome P-450cam versus Temperature, Pressure, and Viscosity" @default.
• W3098796618 doi "https://doi.org/10.1093/oso/9780195097221.003.0015" @default.
• W3098796618 hasPublicationYear "1996" @default.
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