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- W3099305598 abstract "mRNAs and lncRNAs assemble with RNA-binding proteins (RBPs) to form ribonucleoprotein complexes (RNPs ). The assembly of RNPs initiates co-transcriptionally, and their composition and organization is thought to change during the different steps of an RNP life cycle. Modulation of RNP structural organization has been implicated in the regulation of different aspects of RNA metabolism, including establishing interactions between the 5' and 3' ends in regulating mRNA translation and turnover. In this chapter, we describe a single-molecule microscopy approach that combines fluorescent RNA in situ hybridization (smFISH) and structured illumination microscopy (SIM ) and allows to measure different aspects of RNP organization in cells, including distances between different regions within individual mRNAs, as well as the overall compaction state of RNAs in different subcellular compartments and environmental conditions. Moreover, we describe a detailed workflow required for image registration and analysis that allows determining distances at sub-diffraction resolution." @default.
- W3099305598 created "2020-11-23" @default.
- W3099305598 creator A5006554991 @default.
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- W3099305598 date "2020-11-18" @default.
- W3099305598 modified "2023-10-02" @default.
- W3099305598 title "Probing the Conformational State of mRNPs Using smFISH and SIM" @default.
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- W3099305598 doi "https://doi.org/10.1007/978-1-0716-0935-4_17" @default.
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- W3099305598 hasPublicationYear "2020" @default.
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