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• W3100044677 abstract "Abstract Staphylococcus aureus is a leading cause of both acute and chronic infections in humans. Its ability to persist within host cells is thought to play an important role in chronicity and treatment failures. The importance of the pentose phosphate pathway (PPP) during S. aureus chronic infection is currently largely unexplored. Here, we focused on one key PPP enzyme, transketolase. We showed that inactivation of the unique gene encoding transketolase activity in S. aureus USA300 (Δ tkt ) led to an impaired growth in broth. Using time-lapse video imaging, we correlated this phenotype with a defect in early intracellular proliferation compared to wild-type strain. As determined by metabolomic analysis, tkt inactivation also had an important impact on S. aureus metabolism. We then monitored long-term intracellular persistence over 10 days by counting of viable bacteria. Unexpectedly for such a slow-growing strain, the Δ tkt mutant was almost completely eliminated by endothelial cells after ten days, as opposed to a prototypical slow-growing Δ hemDBL mutant for which we recovered 1,000 fold more viable bacteria. We found that in infected cells, the transcriptional activity of the two master regulators Sigma B and RpiRc was drastically reduced in the Δ tkt mutant compared to wild-type strain. Concomitantly, RNAIII transcription was strongly increased. This transcriptional profile is likely to explain the inability of this slow-growing mutant to sustain long-term intracellular survival, suggesting that TKT -or a functional PPP-is required for intracellular bacteria to enable a transcriptional program geared towards persistence. Importance Staphylococcus aureus is a leading cause of severe bacterial infections. This bacterium is readily internalized by non-professional phagocytes and infected cells have been proposed to play an important role in chronic infections and treatment failures. Here, we show the importance of the unique transketolase TKT of S. aureus USA300 in bacterial adaptation during chronic intracellular infection. We show that TKT is mandatory for the metabolomic homeostasis of S. aureus during intracellular persistence. This work unravels the critical role of TKT in the transcriptional regulation of the master regulators Sigma B, RpiRc and RNAIII linking the pentose phosphate pathway to the control of chronic S. aureus infections." @default.
• W3100044677 created "2020-11-23" @default.
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• W3100044677 date "2019-02-02" @default.
• W3100044677 modified "2023-10-18" @default.
• W3100044677 title "Transketolase is involved in the control of Sigma B during chronic infection by<i>Staphylococcus aureus</i>" @default.
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• W3100044677 cites W1493787054 @default.
• W3100044677 cites W1673751863 @default.
• W3100044677 cites W1716237860 @default.
• W3100044677 cites W1944511449 @default.
• W3100044677 cites W1948355459 @default.
• W3100044677 cites W1978163337 @default.
• W3100044677 cites W1978934797 @default.
• W3100044677 cites W1981748478 @default.
• W3100044677 cites W1983193570 @default.
• W3100044677 cites W1983327843 @default.
• W3100044677 cites W1984113256 @default.
• W3100044677 cites W1988020173 @default.
• W3100044677 cites W1999784075 @default.
• W3100044677 cites W2002766407 @default.
• W3100044677 cites W2014813470 @default.
• W3100044677 cites W2016627657 @default.
• W3100044677 cites W2027373007 @default.
• W3100044677 cites W2027622038 @default.
• W3100044677 cites W2030962177 @default.
• W3100044677 cites W2033343409 @default.
• W3100044677 cites W2043655083 @default.
• W3100044677 cites W2048425837 @default.
• W3100044677 cites W2055411163 @default.
• W3100044677 cites W2067801473 @default.
• W3100044677 cites W2082267277 @default.
• W3100044677 cites W2087881133 @default.
• W3100044677 cites W2092487647 @default.
• W3100044677 cites W2093643135 @default.
• W3100044677 cites W2101153968 @default.
• W3100044677 cites W2102177890 @default.
• W3100044677 cites W2105090882 @default.
• W3100044677 cites W2106430848 @default.
• W3100044677 cites W2108551918 @default.
• W3100044677 cites W2116345996 @default.
• W3100044677 cites W2116607488 @default.
• W3100044677 cites W2118617073 @default.
• W3100044677 cites W2120257409 @default.
• W3100044677 cites W2120753451 @default.
• W3100044677 cites W2124908338 @default.
• W3100044677 cites W2128329047 @default.
• W3100044677 cites W2134765452 @default.
• W3100044677 cites W2137895763 @default.
• W3100044677 cites W2141562167 @default.
• W3100044677 cites W2144240948 @default.
• W3100044677 cites W2146327400 @default.
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• W3100044677 cites W2151811178 @default.
• W3100044677 cites W2161096570 @default.
• W3100044677 cites W2206942156 @default.
• W3100044677 cites W2218673609 @default.
• W3100044677 cites W2322749552 @default.
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• W3100044677 cites W2344857049 @default.
• W3100044677 cites W2347124878 @default.
• W3100044677 cites W2412499511 @default.
• W3100044677 cites W2460791328 @default.
• W3100044677 cites W2466153628 @default.
• W3100044677 cites W2466306576 @default.
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• W3100044677 doi "https://doi.org/10.1101/538900" @default.
• W3100044677 hasPublicationYear "2019" @default.
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