FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100104921> ?p ?o ?g. }

• W3100104921 abstract "<h3>Background</h3> Persistence of SARS-CoV-2 virus particles in recovered COVID-19 patients remains a challenge as we continue to fight the ongoing pandemic. For instance, despite three negative consecutive nasopharyngeal swab PCR tests, residual SARS-CoV-2 was reported in the lungs of a deceased patient.¹ Moreover, viral RNA could also be detected in rectal tissues that were obtained during incubation period.² To date, there is no data regarding residual viral particles present in tissues from recovered COVID-19 patients. Hereby, we reported our findings of SARS-CoV-2 viral antigen in liver tissues from a recovered COVID-19 patient. These findings raise concern for potential transmissibility in recovered individuals. <h3>Methods</h3> A 49-year-old South Asian male diagnosed with COVID-19 in June 2020, with incidental discovery of hepatitis B virus (HBV)-associated R0 Grade 2 hepatocellular carcinoma (HCC), was consented for our study. He did not develop significant acute respiratory symptoms throughout the course of the disease. He underwent curative resection of HCC 85 days after being tested COVID-19 negative where his blood, normal tissue and tumour samples were obtained for further analysis (figure 1). We performed deep immunopathological profiling on the specimens using multiplex immunohistochemistry and 25-colour flow cytometry to study SARS-CoV-2-elicited immune response. <h3>Results</h3> Multiplex immunohistochemistry detected SARS-CoV-2 nucleocapsid protein only in adjacent normal liver tissue but not within tumour core (figure 2). We also observed SARS-CoV-2 in some immune cells such as sinusoidal Kupffer cells (figure 2). Additionally, upon stimulation with SARS-CoV-2 peptides, we successfully elicited SARS-CoV-2-specific memory response which is distinct from the response upon challenge with HBV peptides. These findings were similar to our previous discovery in a patient with colorectal adenocarcinoma where we have shown viral antigen detection, validated with PCR to detect viral RNA, as well as the detection of SARS-CoV-2 memory-like T cells in situ (figure 2). Deep profiling of the samples is on-going with single-cell analysis and digital spatial profiling. <h3>Conclusions</h3> We believe this is the first immune profiling report of the in situ tumour microenvironment in a cancer patient with COVID-19. Our findings demonstrated the presence of viral proteins in the liver despite negative swab test result and the ability to elicit ex vivo SARS-CoV-2-specific immune responses through peptide stimulation assays. We also detected same immune cell phenotypes in situ in the cancer tissues. Taken together, we propose caution when handling tissues from patients who have a recent history of COVID-19, particularly during aerosol-generating procedures such as ultrasonic dissection surgery. <h3>Ethics Approval</h3> This study was approved by Centralised Institutional Review Board of SingHealth, approval number 2019/2653. <h3>Consent</h3> Written informed consent was obtained from the patient for publication of this abstract and any accompanying images. A copy of the written consent is available for review by the Editor of this journal. <h3>References</h3> Yao XH, He ZC, Li TY, Zhang HR, Wang Y, Mou H, et al. Pathological evidence for residual SARS-CoV-2 in pulmonary tissues of a ready-for-discharge patient. <i>Cell Res</i> 2020;30(6):541-3. Qian Q, Fan L, Liu W, Li J, Yue J, Wang M, et al. Direct evidence of active SARS-CoV-2 replication in the intestine. <i>Clin Infect Dis</i> 2020." @default.
• W3100104921 created "2020-11-23" @default.
• W3100104921 creator A5001982254 @default.
• W3100104921 creator A5005856890 @default.
• W3100104921 creator A5006203193 @default.
• W3100104921 creator A5009795603 @default.
• W3100104921 creator A5012578914 @default.
• W3100104921 creator A5028143580 @default.
• W3100104921 creator A5037454628 @default.
• W3100104921 creator A5038127214 @default.
• W3100104921 creator A5040748674 @default.
• W3100104921 creator A5044561452 @default.
• W3100104921 creator A5046785035 @default.
• W3100104921 creator A5059298167 @default.
• W3100104921 creator A5069464289 @default.
• W3100104921 creator A5078632387 @default.
• W3100104921 creator A5082613416 @default.
• W3100104921 creator A5085352143 @default.
• W3100104921 creator A5088429762 @default.
• W3100104921 date "2020-11-01" @default.
• W3100104921 modified "2023-10-02" @default.
• W3100104921 title "825 Deep immune profiling of SARS-CoV-2 associated immune microenvironment in cancer tissues from recovered COVID-19 patients" @default.
• W3100104921 doi "https://doi.org/10.1136/jitc-2020-sitc2020.0825" @default.
• W3100104921 hasPublicationYear "2020" @default.
• W3100104921 type Work @default.
• W3100104921 sameAs 3100104921 @default.
• W3100104921 citedByCount "0" @default.
• W3100104921 crossrefType "proceedings-article" @default.
• W3100104921 hasAuthorship W3100104921A5001982254 @default.
• W3100104921 hasAuthorship W3100104921A5005856890 @default.
• W3100104921 hasAuthorship W3100104921A5006203193 @default.
• W3100104921 hasAuthorship W3100104921A5009795603 @default.
• W3100104921 hasAuthorship W3100104921A5012578914 @default.
• W3100104921 hasAuthorship W3100104921A5028143580 @default.
• W3100104921 hasAuthorship W3100104921A5037454628 @default.
• W3100104921 hasAuthorship W3100104921A5038127214 @default.
• W3100104921 hasAuthorship W3100104921A5040748674 @default.
• W3100104921 hasAuthorship W3100104921A5044561452 @default.
• W3100104921 hasAuthorship W3100104921A5046785035 @default.
• W3100104921 hasAuthorship W3100104921A5059298167 @default.
• W3100104921 hasAuthorship W3100104921A5069464289 @default.
• W3100104921 hasAuthorship W3100104921A5078632387 @default.
• W3100104921 hasAuthorship W3100104921A5082613416 @default.
• W3100104921 hasAuthorship W3100104921A5085352143 @default.
• W3100104921 hasAuthorship W3100104921A5088429762 @default.
• W3100104921 hasBestOaLocation W31001049211 @default.
• W3100104921 hasConcept C126322002 @default.
• W3100104921 hasConcept C142724271 @default.
• W3100104921 hasConcept C159047783 @default.
• W3100104921 hasConcept C159654299 @default.
• W3100104921 hasConcept C203014093 @default.
• W3100104921 hasConcept C204232928 @default.
• W3100104921 hasConcept C2522874641 @default.
• W3100104921 hasConcept C2778019345 @default.
• W3100104921 hasConcept C2781188995 @default.
• W3100104921 hasConcept C534529494 @default.
• W3100104921 hasConcept C60644358 @default.
• W3100104921 hasConcept C71924100 @default.
• W3100104921 hasConcept C86803240 @default.
• W3100104921 hasConcept C8891405 @default.
• W3100104921 hasConceptScore W3100104921C126322002 @default.
• W3100104921 hasConceptScore W3100104921C142724271 @default.
• W3100104921 hasConceptScore W3100104921C159047783 @default.
• W3100104921 hasConceptScore W3100104921C159654299 @default.
• W3100104921 hasConceptScore W3100104921C203014093 @default.
• W3100104921 hasConceptScore W3100104921C204232928 @default.
• W3100104921 hasConceptScore W3100104921C2522874641 @default.
• W3100104921 hasConceptScore W3100104921C2778019345 @default.
• W3100104921 hasConceptScore W3100104921C2781188995 @default.
• W3100104921 hasConceptScore W3100104921C534529494 @default.
• W3100104921 hasConceptScore W3100104921C60644358 @default.
• W3100104921 hasConceptScore W3100104921C71924100 @default.
• W3100104921 hasConceptScore W3100104921C86803240 @default.
• W3100104921 hasConceptScore W3100104921C8891405 @default.
• W3100104921 hasLocation W31001049211 @default.
• W3100104921 hasOpenAccess W3100104921 @default.
• W3100104921 hasPrimaryLocation W31001049211 @default.
• W3100104921 hasRelatedWork W1972800817 @default.
• W3100104921 hasRelatedWork W2016052042 @default.
• W3100104921 hasRelatedWork W2016551366 @default.
• W3100104921 hasRelatedWork W2139983200 @default.
• W3100104921 hasRelatedWork W2302132233 @default.
• W3100104921 hasRelatedWork W2353812755 @default.
• W3100104921 hasRelatedWork W2360163270 @default.
• W3100104921 hasRelatedWork W2557491197 @default.
• W3100104921 hasRelatedWork W2766588635 @default.
• W3100104921 hasRelatedWork W3140247098 @default.
• W3100104921 isParatext "false" @default.
• W3100104921 isRetracted "false" @default.
• W3100104921 magId "3100104921" @default.
• W3100104921 workType "article" @default.