FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100205744> ?p ?o ?g. }

• W3100205744 abstract "Abstract Background Chemicals in disparate structural classes activate specific subsets of PPARγ’s transcriptional programs to generate adipocytes with distinct phenotypes. Objectives Our objectives were to 1) establish a novel classification method to predict PPARγ ligands and modifying chemicals, and 2) create a taxonomy to group chemicals based on their effects on PPARγ’s transcriptome and downstream metabolic functions. We tested the hypothesis that environmental adipogens highly ranked by the taxonomy, but segregated from therapeutic PPARγ ligands, would induce white but not brite adipogenesis. Methods 3T3-L1 cells were differentiated in the presence of 76 chemicals (negative controls, nuclear receptor ligands known to influence adipocyte biology, potential environmental PPARγ ligands). Differentiation was assessed by measuring lipid accumulation. mRNA expression was determined by RNA-Seq and validated by RT-qPCR. A novel classification model was developed using an amended random forest procedure. A subset of environmental contaminants identified as strong PPARγ agonists were analyzed by their effects on lipid handling, mitochondrial biogenesis and cellular respiration in 3T3-L1 cells and human preadipocytes. Results We used lipid accumulation and RNA sequencing data to develop a classification system that 1) identified PPARγ agonists, and 2) sorted chemicals into likely white or brite adipogens. Expression of Cidec was the most efficacious indicator of strong PPARγ activation. Two known environmental PPARγ ligands, tetrabromobisphenol A and triphenyl phosphate, which sorted distinctly from therapeutic ligands, induced white adipocyte genes but failed to induce Pgc1a and Ucp1 , and induced fatty acid uptake but not mitochondrial biogenesis in 3T3-L1 cells. Moreover, two chemicals identified as highly ranked PPARγ agonists, tonalide and quinoxyfen, induced white adipogenesis without the concomitant health-promoting characteristics of brite adipocytes in mouse and human preadipocytes. Discussion A novel classification procedure accurately identified environmental chemicals as PPARγ ligands distinct from known PPARγ-activating therapeutics. The computational and experimental framework has general applicability to the classification of as-yet uncharacterized chemicals." @default.
• W3100205744 created "2020-11-23" @default.
• W3100205744 creator A5006919022 @default.
• W3100205744 creator A5015152982 @default.
• W3100205744 creator A5081435946 @default.
• W3100205744 creator A5081898124 @default.
• W3100205744 date "2019-01-13" @default.
• W3100205744 modified "2023-09-27" @default.
• W3100205744 title "A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite Adipogens" @default.
• W3100205744 cites W1425268590 @default.
• W3100205744 cites W1492870075 @default.
• W3100205744 cites W1541679260 @default.
• W3100205744 cites W1552214119 @default.
• W3100205744 cites W1636205509 @default.
• W3100205744 cites W1971194701 @default.
• W3100205744 cites W1971371724 @default.
• W3100205744 cites W1972976212 @default.
• W3100205744 cites W1989209932 @default.
• W3100205744 cites W1989468848 @default.
• W3100205744 cites W1989736679 @default.
• W3100205744 cites W1992326632 @default.
• W3100205744 cites W1993102144 @default.
• W3100205744 cites W1994109490 @default.
• W3100205744 cites W2000106816 @default.
• W3100205744 cites W2001306199 @default.
• W3100205744 cites W2001657480 @default.
• W3100205744 cites W2002519246 @default.
• W3100205744 cites W2004861205 @default.
• W3100205744 cites W2005674716 @default.
• W3100205744 cites W2010457001 @default.
• W3100205744 cites W2011730239 @default.
• W3100205744 cites W2012133139 @default.
• W3100205744 cites W2030322782 @default.
• W3100205744 cites W2052336882 @default.
• W3100205744 cites W2052609811 @default.
• W3100205744 cites W2055494790 @default.
• W3100205744 cites W2056250382 @default.
• W3100205744 cites W2058053854 @default.
• W3100205744 cites W2063163418 @default.
• W3100205744 cites W2087613795 @default.
• W3100205744 cites W2088986234 @default.
• W3100205744 cites W2097127734 @default.
• W3100205744 cites W2101460040 @default.
• W3100205744 cites W2103761194 @default.
• W3100205744 cites W2108244474 @default.
• W3100205744 cites W2112936536 @default.
• W3100205744 cites W2118055379 @default.
• W3100205744 cites W2120447865 @default.
• W3100205744 cites W2123498365 @default.
• W3100205744 cites W2126292889 @default.
• W3100205744 cites W2128308857 @default.
• W3100205744 cites W2130410032 @default.
• W3100205744 cites W2134880599 @default.
• W3100205744 cites W2135009079 @default.
• W3100205744 cites W2146512944 @default.
• W3100205744 cites W2148824946 @default.
• W3100205744 cites W2149039334 @default.
• W3100205744 cites W2159707944 @default.
• W3100205744 cites W2164628019 @default.
• W3100205744 cites W2170245391 @default.
• W3100205744 cites W2239740168 @default.
• W3100205744 cites W2260670291 @default.
• W3100205744 cites W2298475122 @default.
• W3100205744 cites W2332016763 @default.
• W3100205744 cites W2344167399 @default.
• W3100205744 cites W2411583672 @default.
• W3100205744 cites W2415443789 @default.
• W3100205744 cites W2473078753 @default.
• W3100205744 cites W2474750270 @default.
• W3100205744 cites W2515574180 @default.
• W3100205744 cites W2527388867 @default.
• W3100205744 cites W2562244434 @default.
• W3100205744 cites W2583152260 @default.
• W3100205744 cites W2587383738 @default.
• W3100205744 cites W2589775300 @default.
• W3100205744 cites W2592162250 @default.
• W3100205744 cites W2612467560 @default.
• W3100205744 cites W2614122606 @default.
• W3100205744 cites W2634393565 @default.
• W3100205744 cites W2735376054 @default.
• W3100205744 cites W2766877245 @default.
• W3100205744 cites W2801215756 @default.
• W3100205744 cites W2803220010 @default.
• W3100205744 cites W2807127529 @default.
• W3100205744 cites W2886346360 @default.
• W3100205744 cites W2888677496 @default.
• W3100205744 cites W2906742543 @default.
• W3100205744 cites W2911964244 @default.
• W3100205744 cites W2949350745 @default.
• W3100205744 cites W3029874317 @default.
• W3100205744 cites W3043449991 @default.
• W3100205744 cites W3095729521 @default.
• W3100205744 cites W4238992151 @default.
• W3100205744 cites W4245436919 @default.
• W3100205744 doi "https://doi.org/10.1101/519629" @default.
• W3100205744 hasPublicationYear "2019" @default.
• W3100205744 type Work @default.
• W3100205744 sameAs 3100205744 @default.
• W3100205744 citedByCount "1" @default.
• W3100205744 countsByYear W31002057442020 @default.

• next