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• W3100266980 abstract "Abstract Growth rate is a near-universal selective pressure across microbial species. High growth rates require hundreds of metabolic enzymes, each with different nonlinear kinetics, to be precisely tuned within the bounds set by physicochemical constraints. Yet, the metabolic behaviour of many species is characterized by simple relations between growth rate, enzyme expression levels and metabolic rates. We asked if this simplicity could be the outcome of optimisation by evolution. Indeed, when the growth rate is maximized –in a static environment under mass-conservation and enzyme expression constraints– we prove mathematically that the resulting optimal metabolic flux distribution is described by a limited number of subnetworks, known as Elementary Flux Modes (EFMs). We show that, because EFMs are the minimal subnetworks leading to growth, a small active number automatically leads to the simple relations that are measured. We find that the maximal number of flux-carrying EFMs is determined only by the number of imposed constraints on enzyme expression, not by the size, kinetics or topology of the network. This minimal-EFM extremum principle is illustrated in a graphical framework, which explains qualitative changes in microbial behaviours, such as overflow metabolism and co-consumption, and provides a method for identification of the enzyme expression constraints that limit growth under the prevalent conditions. The extremum principle applies to all microorganisms that are selected for maximal growth rates under protein concentration constraints, for example the solvent capacities of cytosol, membrane or periplasmic space. Author summary The microbial genome encodes for a large network of enzyme-catalyzed reactions. The reaction rates depend on concentrations of enzymes and metabolites, which in turn depend on those rates. Cells face a number of biophysical constraints on enzyme expression, for example due to a limited membrane area or cytosolic volume. Considering this complexity and nonlinearity of metabolism, how is it possible, that experimental data can often be described by simple linear models? We show that it is evolution itself that selects for simplicity. When reproductive rate is maximised, the number of active independent metabolic pathways is bounded by the number of growth-limiting enzyme constraints, which is typically small. A small number of pathways automatically generates the measured simple relations. We identify the importance of growth-limiting constraints in shaping microbial behaviour, by focussing on their mechanistic nature. We demonstrate that overflow metabolism – an important phenomenon in bacteria, yeasts, and cancer cells – is caused by two constraints on enzyme expression. We derive experimental guidelines for constraint identification in microorganisms. Knowing these constraints leads to increased understanding of metabolism, and thereby to better predictions and more effective manipulations." @default.
• W3100266980 created "2020-11-23" @default.
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• W3100266980 date "2017-07-21" @default.
• W3100266980 modified "2023-10-03" @default.
• W3100266980 title "The number of active metabolic pathways is bounded by the number of cellular constraints at maximal metabolic rates" @default.
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• W3100266980 doi "https://doi.org/10.1101/167171" @default.
• W3100266980 hasPublicationYear "2017" @default.
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