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- W310027188 abstract "Previous experiments under laboratory conditions on ectomycorrhizal inoculation of Hopea odorata and //. helferi have shown that heavy infection could be obtained with corresponding increase in shoot dry weight by up to 7.3 times and increased foliar phosphorus concentration by about 2 times (Yazid et al. 1994). The experiments were conducted using cardboard inoculum (Chilvers et al. 1986) which is a convenient technique for screening potential mycorrhizal fungal strains. However, a prerequisite for the produc tion of high quality mycorrhizal inoculated dipterocarp seedlings is the use of other forms of inoculum which can be mass produced cheaply and easily. A form of inoculum which is widely used in the temperate regions for inoculation of seedlings of forest tree species is a peat-moss: vermiculite mixture mixed with a suitable nutrient medium and colonised by the hyphae of selected ectomycorrhizal fungi. Thus this study was carried out to investigate the potential of a similar type of inoculum for inoculation of dipterocarp seedlings. Two hundred millilitres of modified Melin-Norkrans medium (Marx & Bryan 1975) was added to a 300 ml mixture of chopped coconut husk fibre and vermiculite (1:9) (vermiculite inoculum) in glassjars. Coconut husk was sucessfully substituted for peatmoss which is more readily available in temperate region nurseries. This substrate was then autoclaved for 20 min at 120 °C. On cooling, the substrate was inoculated with 10 plugs (5 mm diameter) of the test fungus which had been raised on agar medium (potato dextrose agar) for two weeks. Pisolilhus tinctorius strain Pt441 obtained from a carpophore collected in Brazil under Eucalyptus citriodora by M.H. Ivory was used as the test fungus. The inoculatedjars were kept at ambient room temperature (about 28 °C) for between 6 and 8 weeks until complete colonisation of the substrate had occurred. Four-week-old seedlings of Hopea odorata Roxb. germinated in sand were transplanted into polybags containing approximately 500 g of a steam sterilised forest soihsand mixture (3:1) (Yazid et al. 1994). Before transplanting, the potting substrate was inoculated by adding 20% v/v of the actively growing mycorrhizal inoculum. Two control treatments were used: CI which consisted of uninoculated potting susbtrate and C2 with the autoclaved fungal inoculum mixed in the same proportion as in the inoculated treatment. The seedlings, 15 replicates per treatment, were kept for 6 months in a shade house with 37% relative light intensity." @default.
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- W310027188 date "1996-01-01" @default.
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- W310027188 title "Mycorrhizal inoculation of Hopea odorata (Dipterocarpaceae) in the nursery." @default.
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