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- W31002994 abstract "A rapid method for electrophoresis of DNA-protein complexes is described. Popular gel-shift assays are performed using Pharmacia PhastSystem with its convenience of pre-cast gels and buffer strips and microprocessor-controlled high-resolution separation. Using this system, the products of a DNA binding reaction (DNA-protein complexes) can be separated from free DNA in less than one hour. DNA fragments as well as oligonucleotides have been used as targets with partially purified extracts containing sequence-specific DNA binding proteins. We present here a comparison of results of gel-shift assays obtained by conventional techniques and by our rapid PhastShift method which reduces the time, effort and technical expertise necessary to obtain reproducible results." @default.
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- W31002994 date "1990-05-01" @default.
- W31002994 modified "2023-09-25" @default.
- W31002994 title "Fast gel electrophoresis to analyze DNA-protein interactions." @default.
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