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- W3100299743 abstract "Abstract The roles of protein conformational dynamics and allostery in function are well-known. However, the roles that inter-domain dynamics have in function are not entirely understood. We used matrix metalloprotease-1 (MMP1) as a model system to study the relationship between inter-domain dynamics and activity because MMP1 has diverse substrates. Here we focus on fibrin, the primary component of a blood clot. Water-soluble fibrinogen, following cleavage by thrombin, self-polymerize to form water-insoluble fibrin. We studied the inter-domain dynamics of MMP1 on fibrin without crosslinks using single-molecule Forster Resonance Energy Transfer (smFRET). We observed that the distance between the catalytic and hemopexin domains of MMP1 increases or decreases as the MMP1 activity increases or decreases, respectively. We modulated the activity using 1) an active site mutant (E219Q) of MMP1, 2) MMP9, another member of the MMP family that increases the activity of MMP1, and 3) tetracycline, an inhibitor of MMP1. We fitted the histograms of smFRET values to a sum of two Gaussians and the autocorrelations to an exponential and power law. We modeled the dynamics as a two-state Poisson process and calculated the kinetic rates from the histograms and autocorrelations. Activity-dependent inter-domain dynamics may enable allosteric control of the MMP1 function." @default.
- W3100299743 created "2020-11-23" @default.
- W3100299743 creator A5017682510 @default.
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- W3100299743 date "2019-11-24" @default.
- W3100299743 modified "2023-10-18" @default.
- W3100299743 title "Inter-domain dynamics and fibrinolytic activity of matrix metalloprotease-1" @default.
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- W3100299743 doi "https://doi.org/10.1101/853796" @default.
- W3100299743 hasPublicationYear "2019" @default.
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