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• W3100302710 endingPage "102017" @default.
• W3100302710 startingPage "102017" @default.
• W3100302710 abstract "A flow cytometric assay measuring Mycobacterium tuberculosis-specific CD4 T-cell responses using co-expression of CD25/CD134 (OX40 assay) was explored as a diagnostic tool for active tuberculosis (TB) in a Thai population with and without HIV infection. Peripheral blood mononuclear cells (PBMC) obtained from 133 participants at TB diagnosis were cryopreserved. Seventy-six participants had a clinical diagnosis of TB which were confirmed by a positive culture. CD4 T-cell responses were measured after stimulation with a pool of overlapping peptides covering RD-1 antigens: CFP-10 + ESAT-6. The performance of the assay was also compared to the Xpert MTB/RIF assay. The overall sensitivity of the OX40 assay was 94.7% (95%CI 87.1-98.5); its specificity was 71.9% (95%CI, 58.5-83). The sensitivity of the OX40 assay among HIV-infected participants was 100% (95%CI, 88.8-100) with a specificity of 92.9% (95%CI, 66.1-99.8). OX40 assay performed particularly well in those with active TB and HIV infection." @default.
• W3100302710 created "2020-11-23" @default.
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• W3100302710 date "2021-01-01" @default.
• W3100302710 modified "2023-10-18" @default.
• W3100302710 title "Performance of a simple flow cytometric assay in diagnosing active tuberculosis" @default.
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• W3100302710 doi "https://doi.org/10.1016/j.tube.2020.102017" @default.
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