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- W3100308299 abstract "ABSTRACT All membrane fusion reactions proceed through an initial fusion pore, including calcium-triggered release of neurotransmitters and hormones. Expansion of this small pore to release cargo is energetically costly and regulated by cells, but the mechanisms are poorly understood. Here we show that the neuronal/exocytic calcium sensor Synaptotagmin-1 (Syt1) promotes expansion of fusion pores induced by SNARE proteins. Pore dilation relied on calcium-induced insertion of the tandem C2 domain hydrophobic loops of Syt1 into the membrane, previously shown to reorient the C2 domain. Mathematical modelling suggests that C2B reorientation rotates a bound SNARE complex so that it exerts force on the membranes in a mechanical lever action that increases the height of the fusion pore, provoking pore dilation to offset the bending energy penalty. We conclude that Syt1 exerts novel non-local calcium-dependent mechanical forces on fusion pores that dilate pores and assist neurotransmitter and hormone release. SIGNIFICANCE STATEMENT During neurotransmitter release, calcium-induced membrane insertion of the C2B domain of Synaptotagmin re-orients the bound SNARE complex which dilates the fusion pore in a mechanical lever action." @default.
- W3100308299 created "2020-11-23" @default.
- W3100308299 creator A5059017907 @default.
- W3100308299 creator A5062845468 @default.
- W3100308299 creator A5086526072 @default.
- W3100308299 creator A5087849965 @default.
- W3100308299 creator A5088931483 @default.
- W3100308299 date "2019-04-30" @default.
- W3100308299 modified "2023-10-13" @default.
- W3100308299 title "The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores" @default.
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