Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100369134> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W3100369134 abstract "Abstract Coronavirus possesses the largest RNA genome among all the RNA viruses. Its genome encodes about 29 proteins. Most of the viral proteins are non-structural proteins (NSP) except envelop (E), membrane (M), nucleocapsid (N) and Spike (S) proteins that constitute the viral nucleocapsid, envelop and surface. We have recently cloned all the 29 SARS-CoV-2 genes into vectors for their expressions in mammalian cells except NSP11 that has only 14 amino acids (aa). We are able to express all the 28 cloned SARS-CoV-2 genes in human cells to characterize their subcellular distributions. The proteins of SARS-CoV-2 are mostly cytoplasmic but some are both cytoplasmic and nuclear. Those punctate staining proteins were further investigated by immunofluorescent assay (IFA) using specific antibodies or by co-transfection with an organelle marker-expressing plasmid. As a result, we found that NSP15, ORF6, M and ORF7a are related to Golgi apparatus, and that ORF7b, ORF8 and ORF10 colocalize with endoplasmic reticulum (ER). Interestingly, ORF3a distributes in cell membrane, early endosome, endosome, late endosome and lysosome, which suggests that ORF3a might help the infected virus to usurp endosome and lysosome for viral use. Furthermore, we revealed that NSP13 colocalized with SC35, a protein standing for splicing compartments in the nucleus. Our studies for the first time visualized the subcellular locations of SARS-CoV-2 proteins and might provide novel insights into the viral proteins’ biological functions." @default.
- W3100369134 created "2020-11-23" @default.
- W3100369134 creator A5000500229 @default.
- W3100369134 creator A5017433297 @default.
- W3100369134 creator A5031905995 @default.
- W3100369134 creator A5035517104 @default.
- W3100369134 creator A5043873595 @default.
- W3100369134 creator A5044371180 @default.
- W3100369134 creator A5046214153 @default.
- W3100369134 creator A5081951768 @default.
- W3100369134 date "2020-11-17" @default.
- W3100369134 modified "2023-10-10" @default.
- W3100369134 title "A systemic and molecular study of subcellular localization of SARS-CoV-2 proteins" @default.
- W3100369134 cites W1994668150 @default.
- W3100369134 cites W2067669402 @default.
- W3100369134 cites W2168080864 @default.
- W3100369134 cites W2734954484 @default.
- W3100369134 cites W3048171632 @default.
- W3100369134 doi "https://doi.org/10.1038/s41392-020-00372-8" @default.
- W3100369134 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7670843" @default.
- W3100369134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33203855" @default.
- W3100369134 hasPublicationYear "2020" @default.
- W3100369134 type Work @default.
- W3100369134 sameAs 3100369134 @default.
- W3100369134 citedByCount "97" @default.
- W3100369134 countsByYear W31003691342020 @default.
- W3100369134 countsByYear W31003691342021 @default.
- W3100369134 countsByYear W31003691342022 @default.
- W3100369134 countsByYear W31003691342023 @default.
- W3100369134 crossrefType "journal-article" @default.
- W3100369134 hasAuthorship W3100369134A5000500229 @default.
- W3100369134 hasAuthorship W3100369134A5017433297 @default.
- W3100369134 hasAuthorship W3100369134A5031905995 @default.
- W3100369134 hasAuthorship W3100369134A5035517104 @default.
- W3100369134 hasAuthorship W3100369134A5043873595 @default.
- W3100369134 hasAuthorship W3100369134A5044371180 @default.
- W3100369134 hasAuthorship W3100369134A5046214153 @default.
- W3100369134 hasAuthorship W3100369134A5081951768 @default.
- W3100369134 hasBestOaLocation W31003691341 @default.
- W3100369134 hasConcept C116675565 @default.
- W3100369134 hasConcept C142724271 @default.
- W3100369134 hasConcept C159047783 @default.
- W3100369134 hasConcept C2779134260 @default.
- W3100369134 hasConcept C3006700255 @default.
- W3100369134 hasConcept C3007834351 @default.
- W3100369134 hasConcept C3008058167 @default.
- W3100369134 hasConcept C3020799909 @default.
- W3100369134 hasConcept C524204448 @default.
- W3100369134 hasConcept C70721500 @default.
- W3100369134 hasConcept C71924100 @default.
- W3100369134 hasConcept C86803240 @default.
- W3100369134 hasConceptScore W3100369134C116675565 @default.
- W3100369134 hasConceptScore W3100369134C142724271 @default.
- W3100369134 hasConceptScore W3100369134C159047783 @default.
- W3100369134 hasConceptScore W3100369134C2779134260 @default.
- W3100369134 hasConceptScore W3100369134C3006700255 @default.
- W3100369134 hasConceptScore W3100369134C3007834351 @default.
- W3100369134 hasConceptScore W3100369134C3008058167 @default.
- W3100369134 hasConceptScore W3100369134C3020799909 @default.
- W3100369134 hasConceptScore W3100369134C524204448 @default.
- W3100369134 hasConceptScore W3100369134C70721500 @default.
- W3100369134 hasConceptScore W3100369134C71924100 @default.
- W3100369134 hasConceptScore W3100369134C86803240 @default.
- W3100369134 hasFunder F4320337355 @default.
- W3100369134 hasFunder F4320337365 @default.
- W3100369134 hasFunder F4320337534 @default.
- W3100369134 hasIssue "1" @default.
- W3100369134 hasLocation W31003691341 @default.
- W3100369134 hasLocation W31003691342 @default.
- W3100369134 hasLocation W31003691343 @default.
- W3100369134 hasLocation W31003691344 @default.
- W3100369134 hasOpenAccess W3100369134 @default.
- W3100369134 hasPrimaryLocation W31003691341 @default.
- W3100369134 hasRelatedWork W3087090141 @default.
- W3100369134 hasRelatedWork W3087967611 @default.
- W3100369134 hasRelatedWork W3113664224 @default.
- W3100369134 hasRelatedWork W3127693599 @default.
- W3100369134 hasRelatedWork W3209591042 @default.
- W3100369134 hasRelatedWork W4200329650 @default.
- W3100369134 hasRelatedWork W4206669628 @default.
- W3100369134 hasRelatedWork W4210401150 @default.
- W3100369134 hasRelatedWork W4214661089 @default.
- W3100369134 hasRelatedWork W4289131272 @default.
- W3100369134 hasVolume "5" @default.
- W3100369134 isParatext "false" @default.
- W3100369134 isRetracted "false" @default.
- W3100369134 magId "3100369134" @default.
- W3100369134 workType "article" @default.