FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100394707> ?p ?o ?g. }

• W3100394707 abstract "Recombinant protein production with E. coli is usually carried out in fed-batch mode in industry. As set-up and cleaning of equipment are time- and cost-intensive, it would be economically and environmentally favourable to reduce the number of these procedures. Switching from fed-batch to continuous biomanufacturing with microbials is not yet applied as these cultivations still suffer from time-dependent variations in productivity. Repetitive fed-batch process technology facilitates critical equipment usage, reduces the environmental fingerprint and potentially increases the overall space-time yield. Surprisingly, studies on repetitive fed-batch processes for recombinant protein production can be found for yeasts only. Knowledge on repetitive fed-batch cultivation technology for recombinant protein production in E. coli is not available until now. In this study, a mixed feed approach, enabling repetitive fed-batch technology for recombinant protein production in E. coli, was developed. Effects of the cultivation mode on the space-time yield for a single-cycle fed-batch, a two-cycle repetitive fed-batch-, a three-cycle repetitive fed batch and a chemostat cultivation were investigated. For that purpose, we used two different E. coli strains, expressing a model protein in the cytoplasm or in the periplasm, respectively. Our results demonstrate that a repetitive fed-batch for E. coli leads to a higher space-time yield compared to a single-cycle fed-batch and can potentially outperform continuous biomanufacturing. For the first time, we were able to show that repetitive fed-batch technology is highly suitable for recombinant protein production in E. coli using our mixed feeding approach, as it potentially (i) improves product throughput by using critical equipment to its full capacity and (ii) allows implementation of a more economic process by reducing cleaning and set-up times." @default.
• W3100394707 created "2020-11-23" @default.
• W3100394707 creator A5002747234 @default.
• W3100394707 creator A5017007688 @default.
• W3100394707 creator A5043413230 @default.
• W3100394707 creator A5055332747 @default.
• W3100394707 creator A5065854896 @default.
• W3100394707 creator A5080708528 @default.
• W3100394707 date "2020-11-10" @default.
• W3100394707 modified "2023-09-26" @default.
• W3100394707 title "Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing With E. coli" @default.
• W3100394707 cites W1741223151 @default.
• W3100394707 cites W1860617187 @default.
• W3100394707 cites W1877476777 @default.
• W3100394707 cites W1981092077 @default.
• W3100394707 cites W1987130080 @default.
• W3100394707 cites W1987964160 @default.
• W3100394707 cites W1993402995 @default.
• W3100394707 cites W1998028440 @default.
• W3100394707 cites W2019515050 @default.
• W3100394707 cites W2020186179 @default.
• W3100394707 cites W2041394915 @default.
• W3100394707 cites W2048384187 @default.
• W3100394707 cites W2066816024 @default.
• W3100394707 cites W2073010988 @default.
• W3100394707 cites W2093915148 @default.
• W3100394707 cites W2099354939 @default.
• W3100394707 cites W2101896194 @default.
• W3100394707 cites W2108940138 @default.
• W3100394707 cites W2114641752 @default.
• W3100394707 cites W2121421440 @default.
• W3100394707 cites W2138820253 @default.
• W3100394707 cites W2166374078 @default.
• W3100394707 cites W2200387749 @default.
• W3100394707 cites W2222849530 @default.
• W3100394707 cites W2254304961 @default.
• W3100394707 cites W2346327658 @default.
• W3100394707 cites W2346788556 @default.
• W3100394707 cites W2349904868 @default.
• W3100394707 cites W2400812064 @default.
• W3100394707 cites W2401115505 @default.
• W3100394707 cites W2409426195 @default.
• W3100394707 cites W2416858988 @default.
• W3100394707 cites W2417372989 @default.
• W3100394707 cites W2470436472 @default.
• W3100394707 cites W2510481608 @default.
• W3100394707 cites W2513042356 @default.
• W3100394707 cites W2538225379 @default.
• W3100394707 cites W2600837577 @default.
• W3100394707 cites W2693798264 @default.
• W3100394707 cites W2723535088 @default.
• W3100394707 cites W2734736606 @default.
• W3100394707 cites W2770164654 @default.
• W3100394707 cites W2776422285 @default.
• W3100394707 cites W2788004430 @default.
• W3100394707 cites W2788247942 @default.
• W3100394707 cites W2794306218 @default.
• W3100394707 cites W2794889462 @default.
• W3100394707 cites W2809558679 @default.
• W3100394707 cites W2891897409 @default.
• W3100394707 cites W2894443893 @default.
• W3100394707 cites W2899054191 @default.
• W3100394707 cites W2912105837 @default.
• W3100394707 cites W2920893072 @default.
• W3100394707 cites W2935247848 @default.
• W3100394707 cites W2947501480 @default.
• W3100394707 cites W2952116753 @default.
• W3100394707 cites W2959162769 @default.
• W3100394707 cites W2964298233 @default.
• W3100394707 cites W2986150861 @default.
• W3100394707 cites W2990978502 @default.
• W3100394707 cites W2997456998 @default.
• W3100394707 cites W3016477334 @default.
• W3100394707 cites W3033086268 @default.
• W3100394707 cites W3049314307 @default.
• W3100394707 cites W4319293046 @default.
• W3100394707 doi "https://doi.org/10.3389/fbioe.2020.573607" @default.
• W3100394707 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7683717" @default.
• W3100394707 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33240864" @default.
• W3100394707 hasPublicationYear "2020" @default.
• W3100394707 type Work @default.
• W3100394707 sameAs 3100394707 @default.
• W3100394707 citedByCount "7" @default.
• W3100394707 countsByYear W31003947072021 @default.
• W3100394707 countsByYear W31003947072022 @default.
• W3100394707 countsByYear W31003947072023 @default.
• W3100394707 crossrefType "journal-article" @default.
• W3100394707 hasAuthorship W3100394707A5002747234 @default.
• W3100394707 hasAuthorship W3100394707A5017007688 @default.
• W3100394707 hasAuthorship W3100394707A5043413230 @default.
• W3100394707 hasAuthorship W3100394707A5055332747 @default.
• W3100394707 hasAuthorship W3100394707A5065854896 @default.
• W3100394707 hasAuthorship W3100394707A5080708528 @default.
• W3100394707 hasBestOaLocation W31003947071 @default.
• W3100394707 hasConcept C100544194 @default.
• W3100394707 hasConcept C104317684 @default.
• W3100394707 hasConcept C127413603 @default.
• W3100394707 hasConcept C134121241 @default.
• W3100394707 hasConcept C150903083 @default.
• W3100394707 hasConcept C167431671 @default.

• next