Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100445881> ?p ?o ?g. }
- W3100445881 abstract "Abstract Bioprospecting genes and proteins related to plant biomass degradation is an attractive approach for the identification of target genes for biotechnological purposes, especially genes with potential applications in the biorefinery industry that can enhance second-generation ethanol production technology. Trichoderma harzianum is a potential candidate for cellulolytic enzyme prospection and production. Herein, the enzymatic activities, transcriptome, exoproteome, and coexpression networks of the T. harzianum strain CBMAI-0179 were examined under biomass degradation conditions. We used RNA-Seq to identify differentially expressed genes (DEGs) and carbohydrate-active enzyme (CAZyme) genes related to plant biomass degradation and compared them with the genes of strains from congeneric species ( T. harzianum IOC-3844 and T. atroviride CBMAI-0020). T. harzianum CBMAI-0179 harbors strain- and treatment-specific CAZyme genes and transcription factors. We detected important proteins related to biomass degradation, including β-glucosidases, endoglucanases, cellobiohydrolases, lytic polysaccharide monooxygenases, endo-1,4-β-xylanases and β-mannanases, in the exoproteome under cellulose growth conditions. Coexpression networks were constructed to explore the relationships among the genes and corresponding secreted proteins that act synergistically for cellulose degradation. An enriched cluster with degradative enzymes was described, and the subnetwork of CAZymes revealed strong correlations among the secreted proteins (AA9, GH6, GH10, GH11 and CBM1) and differentially expressed CAZyme genes (GH45, GH7, AA7 and GH1). Our results provide valuable information for future studies on the genetic regulation of plant cell wall-degrading enzymes. This knowledge can be exploited for the improvement of enzymatic reactions in biomass degradation for bioethanol production. Key points Different biotechnological approaches were used to understand the mechanism of cellulose degradation of Trichoderma spp. T. harzianum CBMAI-0179 is a potential candidate for the production of cellulolytic enzymes. Coexpression networks revealed genes and proteins acting synergistically for cellulose hydrolysis." @default.
- W3100445881 created "2020-11-23" @default.
- W3100445881 creator A5028271589 @default.
- W3100445881 creator A5039249840 @default.
- W3100445881 creator A5051720073 @default.
- W3100445881 creator A5077503251 @default.
- W3100445881 creator A5091458015 @default.
- W3100445881 date "2020-01-15" @default.
- W3100445881 modified "2023-09-30" @default.
- W3100445881 title "The synergistic actions of hydrolytic genes reveal the mechanism of Trichoderma harzianum for cellulose degradation" @default.
- W3100445881 cites W1499340485 @default.
- W3100445881 cites W1569360501 @default.
- W3100445881 cites W1842776140 @default.
- W3100445881 cites W1904196773 @default.
- W3100445881 cites W1969337506 @default.
- W3100445881 cites W1995894025 @default.
- W3100445881 cites W2008398710 @default.
- W3100445881 cites W2017519756 @default.
- W3100445881 cites W2019364197 @default.
- W3100445881 cites W2030452223 @default.
- W3100445881 cites W2040760322 @default.
- W3100445881 cites W2065461553 @default.
- W3100445881 cites W2071152129 @default.
- W3100445881 cites W2080310153 @default.
- W3100445881 cites W2082990265 @default.
- W3100445881 cites W2099259718 @default.
- W3100445881 cites W2103017472 @default.
- W3100445881 cites W2103740237 @default.
- W3100445881 cites W2106882534 @default.
- W3100445881 cites W2107277218 @default.
- W3100445881 cites W2122559203 @default.
- W3100445881 cites W2131271579 @default.
- W3100445881 cites W2131959462 @default.
- W3100445881 cites W2148041910 @default.
- W3100445881 cites W2152379610 @default.
- W3100445881 cites W2159675211 @default.
- W3100445881 cites W2162595238 @default.
- W3100445881 cites W2164154943 @default.
- W3100445881 cites W2165574256 @default.
- W3100445881 cites W2171421143 @default.
- W3100445881 cites W2171812124 @default.
- W3100445881 cites W2236822143 @default.
- W3100445881 cites W2274278124 @default.
- W3100445881 cites W2275065560 @default.
- W3100445881 cites W2311203695 @default.
- W3100445881 cites W2328448952 @default.
- W3100445881 cites W2346407364 @default.
- W3100445881 cites W2415318660 @default.
- W3100445881 cites W2440757626 @default.
- W3100445881 cites W2568451401 @default.
- W3100445881 cites W2594074916 @default.
- W3100445881 cites W2606882994 @default.
- W3100445881 cites W2618962964 @default.
- W3100445881 cites W2625419514 @default.
- W3100445881 cites W2740912673 @default.
- W3100445881 cites W2761653020 @default.
- W3100445881 cites W2782555750 @default.
- W3100445881 cites W2799885139 @default.
- W3100445881 cites W2806621462 @default.
- W3100445881 cites W2949926205 @default.
- W3100445881 cites W3022756611 @default.
- W3100445881 cites W4238303038 @default.
- W3100445881 cites W4247649582 @default.
- W3100445881 cites W4254275792 @default.
- W3100445881 cites W4293247451 @default.
- W3100445881 doi "https://doi.org/10.1101/2020.01.14.906529" @default.
- W3100445881 hasPublicationYear "2020" @default.
- W3100445881 type Work @default.
- W3100445881 sameAs 3100445881 @default.
- W3100445881 citedByCount "2" @default.
- W3100445881 countsByYear W31004458812020 @default.
- W3100445881 crossrefType "posted-content" @default.
- W3100445881 hasAuthorship W3100445881A5028271589 @default.
- W3100445881 hasAuthorship W3100445881A5039249840 @default.
- W3100445881 hasAuthorship W3100445881A5051720073 @default.
- W3100445881 hasAuthorship W3100445881A5077503251 @default.
- W3100445881 hasAuthorship W3100445881A5091458015 @default.
- W3100445881 hasBestOaLocation W31004458811 @default.
- W3100445881 hasConcept C100817775 @default.
- W3100445881 hasConcept C104317684 @default.
- W3100445881 hasConcept C104727253 @default.
- W3100445881 hasConcept C150194340 @default.
- W3100445881 hasConcept C150903083 @default.
- W3100445881 hasConcept C162317418 @default.
- W3100445881 hasConcept C181199279 @default.
- W3100445881 hasConcept C2776511177 @default.
- W3100445881 hasConcept C2779251873 @default.
- W3100445881 hasConcept C2780214302 @default.
- W3100445881 hasConcept C2780301381 @default.
- W3100445881 hasConcept C53991642 @default.
- W3100445881 hasConcept C55493867 @default.
- W3100445881 hasConcept C59822182 @default.
- W3100445881 hasConcept C86803240 @default.
- W3100445881 hasConcept C89423630 @default.
- W3100445881 hasConceptScore W3100445881C100817775 @default.
- W3100445881 hasConceptScore W3100445881C104317684 @default.
- W3100445881 hasConceptScore W3100445881C104727253 @default.
- W3100445881 hasConceptScore W3100445881C150194340 @default.
- W3100445881 hasConceptScore W3100445881C150903083 @default.
- W3100445881 hasConceptScore W3100445881C162317418 @default.