Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100470970> ?p ?o ?g. }
- W3100470970 abstract "Summary Cell proliferation changes concomitantly with fate transitions during reprogramming, differentiation, regeneration, and oncogenesis. Methods to resolve cell cycle length heterogeneity in real-time are currently lacking. Here, we describe a genetically encoded fluorescent reporter that captures live cell cycle speed using a single measurement. This reporter is based on the color-changing Fluorescent Timer (FT) protein, which emits blue fluorescence when newly synthesized before maturing into a red fluorescent protein. We generated a mouse strain expressing an H2B-FT fusion reporter from a universally active locus, and demonstrate that faster-cycling cells can be distinguished from slower-cycling ones based on the intracellular fluorescence ratio between the FT’s blue and red states. Using this reporter, we reveal the native cell cycle speed distributions of fresh hematopoietic cells, and demonstrate its utility in analyzing cell proliferation in solid tissues. This system is broadly applicable for dissecting functional heterogeneity associated with cell cycle dynamics in complex tissues." @default.
- W3100470970 created "2020-11-23" @default.
- W3100470970 creator A5009882164 @default.
- W3100470970 creator A5011559137 @default.
- W3100470970 creator A5033793814 @default.
- W3100470970 creator A5039747498 @default.
- W3100470970 creator A5052880489 @default.
- W3100470970 creator A5053715078 @default.
- W3100470970 creator A5062172607 @default.
- W3100470970 creator A5072320963 @default.
- W3100470970 creator A5079150594 @default.
- W3100470970 date "2018-12-11" @default.
- W3100470970 modified "2023-09-23" @default.
- W3100470970 title "Resolving cell cycle speed in one snapshot with a live-cell fluorescent reporter" @default.
- W3100470970 cites W1573024086 @default.
- W3100470970 cites W1577847722 @default.
- W3100470970 cites W1823763033 @default.
- W3100470970 cites W1834426811 @default.
- W3100470970 cites W185571754 @default.
- W3100470970 cites W1911244572 @default.
- W3100470970 cites W1972064898 @default.
- W3100470970 cites W1978938161 @default.
- W3100470970 cites W1982281628 @default.
- W3100470970 cites W1985472914 @default.
- W3100470970 cites W1986378577 @default.
- W3100470970 cites W1988050012 @default.
- W3100470970 cites W1996435746 @default.
- W3100470970 cites W2013329963 @default.
- W3100470970 cites W2013353304 @default.
- W3100470970 cites W2014854196 @default.
- W3100470970 cites W2018011096 @default.
- W3100470970 cites W2027509917 @default.
- W3100470970 cites W2034214363 @default.
- W3100470970 cites W2041255419 @default.
- W3100470970 cites W2042192373 @default.
- W3100470970 cites W2053739797 @default.
- W3100470970 cites W2056109978 @default.
- W3100470970 cites W2058697507 @default.
- W3100470970 cites W2063471926 @default.
- W3100470970 cites W2067129663 @default.
- W3100470970 cites W2069399470 @default.
- W3100470970 cites W2070154483 @default.
- W3100470970 cites W2070809169 @default.
- W3100470970 cites W2076954345 @default.
- W3100470970 cites W2077264573 @default.
- W3100470970 cites W2081777956 @default.
- W3100470970 cites W2083385452 @default.
- W3100470970 cites W2095417588 @default.
- W3100470970 cites W2098710866 @default.
- W3100470970 cites W2098864119 @default.
- W3100470970 cites W2102942399 @default.
- W3100470970 cites W2105495977 @default.
- W3100470970 cites W2106964196 @default.
- W3100470970 cites W2107332774 @default.
- W3100470970 cites W2117961940 @default.
- W3100470970 cites W2121510721 @default.
- W3100470970 cites W2121709505 @default.
- W3100470970 cites W2126686784 @default.
- W3100470970 cites W2130921299 @default.
- W3100470970 cites W2135107819 @default.
- W3100470970 cites W2144518214 @default.
- W3100470970 cites W2146414105 @default.
- W3100470970 cites W2154477046 @default.
- W3100470970 cites W2159082795 @default.
- W3100470970 cites W2159155233 @default.
- W3100470970 cites W2161092951 @default.
- W3100470970 cites W2162723598 @default.
- W3100470970 cites W2169635206 @default.
- W3100470970 cites W2184331293 @default.
- W3100470970 cites W2211016425 @default.
- W3100470970 cites W2272060586 @default.
- W3100470970 cites W2288903023 @default.
- W3100470970 cites W2292396958 @default.
- W3100470970 cites W2295971206 @default.
- W3100470970 cites W2327446251 @default.
- W3100470970 cites W2465250101 @default.
- W3100470970 cites W2511360805 @default.
- W3100470970 cites W2515907404 @default.
- W3100470970 cites W2542820697 @default.
- W3100470970 cites W2557367524 @default.
- W3100470970 cites W2606255398 @default.
- W3100470970 cites W2618711346 @default.
- W3100470970 cites W2623156160 @default.
- W3100470970 cites W2754348497 @default.
- W3100470970 cites W2790203669 @default.
- W3100470970 cites W2800863989 @default.
- W3100470970 cites W2802291136 @default.
- W3100470970 cites W2902352085 @default.
- W3100470970 cites W2951213089 @default.
- W3100470970 cites W3142959081 @default.
- W3100470970 doi "https://doi.org/10.1101/494252" @default.
- W3100470970 hasPublicationYear "2018" @default.
- W3100470970 type Work @default.
- W3100470970 sameAs 3100470970 @default.
- W3100470970 citedByCount "0" @default.
- W3100470970 crossrefType "posted-content" @default.
- W3100470970 hasAuthorship W3100470970A5009882164 @default.
- W3100470970 hasAuthorship W3100470970A5011559137 @default.
- W3100470970 hasAuthorship W3100470970A5033793814 @default.
- W3100470970 hasAuthorship W3100470970A5039747498 @default.