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• W3100489475 endingPage "120" @default.
• W3100489475 startingPage "105" @default.
• W3100489475 abstract "Abstract Glucagon-like peptide-1 (GLP-1) regulates reproduction centrally, although, the neuroanatomical basis of the process is unknown. Therefore, the putative networking of the central GLP-1 and gonadotropin-releasing hormone (GnRH) systems was addressed in male mice using whole mount immunocytochemistry and optogenetics. Enhanced antibody penetration and optical clearing procedures applied to 500–1000 µm thick basal forebrain slices allowed the simultaneous visualization of the two distinct systems in the basal forebrain. Beaded GLP-1-IR axons innervated about a quarter of GnRH neurons (23.2 ± 1.4%) forming either single or multiple contacts. GnRH dendrites received a more intense GLP-1 innervation (64.6 ± 0.03%) than perikarya (35.4 ± 0.03%). The physiological significance of the innervation was examined by optogenetic activation of channelrhodopsin-2 (ChR2)-expressing axons of preproglucagon (GCG) neurons upon the firing of GnRH neurons by patch clamp electrophysiology in acute brain slices of triple transgenic mice (Gcg-cre/ChR2/GFP-GnRH). High-frequency laser beam stimulation (20 Hz, 10 ms pulse width, 3 mW laser power) of ChR2-expressing GCG axons in the mPOA increased the firing rate of GnRH neurons (by 75 ± 17.3%, p = 0.0007). Application of the GLP-1 receptor antagonist, Exendin-3-(9-39) (1 μM), prior to the photo-stimulation, abolished the facilitatory effect. In contrast, low-frequency trains of laser pulses (0.2 Hz, 60 pulses) had no effect on the spontaneous postsynaptic currents of GnRH neurons. The findings indicate a direct wiring of GLP-1 neurons with GnRH cells which route is excitatory for the GnRH system. The pathway may relay metabolic signals to GnRH neurons and synchronize metabolism with reproduction." @default.
• W3100489475 created "2020-11-23" @default.
• W3100489475 creator A5010088706 @default.
• W3100489475 creator A5030181079 @default.
• W3100489475 creator A5030904270 @default.
• W3100489475 creator A5085974441 @default.
• W3100489475 date "2020-11-09" @default.
• W3100489475 modified "2023-10-05" @default.
• W3100489475 title "Networking of glucagon-like peptide-1 axons with GnRH neurons in the basal forebrain of male mice revealed by 3DISCO-based immunocytochemistry and optogenetics" @default.
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• W3100489475 doi "https://doi.org/10.1007/s00429-020-02167-7" @default.
• W3100489475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7817561" @default.
• W3100489475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33169188" @default.
• W3100489475 hasPublicationYear "2020" @default.

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