Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100565477> ?p ?o ?g. }
- W3100565477 abstract "Data-Independent Acquisition (DIA) is a method to improve consistent identification and precise quantitation of peptides and proteins by mass spectrometry (MS). The targeted data analysis strategy in DIA relies on spectral assay libraries that are generally derived from a priori measurements of peptides for each species. Although Escherichia coli (E. coli) is among the best studied model organisms, so far there is no spectral assay library for the bacterium publicly available. Here, we generated a spectral assay library for 4,014 of the 4,389 annotated E. coli proteins using one- and two-dimensional fractionated samples, and ion mobility separation enabling deep proteome coverage. We demonstrate the utility of this high-quality library with robustness in quantitation of the E. coli proteome and with rapid-chromatography to enhance throughput by targeted DIA-MS. The spectral assay library supports the detection and quantification of 91.5% of all E. coli proteins at high-confidence with 56,182 proteotypic peptides, making it a valuable resource for the scientific community. Data and spectral libraries are available via ProteomeXchange (PXD020761, PXD020785) and SWATHAtlas (SAL00222-28)." @default.
- W3100565477 created "2020-11-23" @default.
- W3100565477 creator A5001209740 @default.
- W3100565477 creator A5005287667 @default.
- W3100565477 creator A5010894905 @default.
- W3100565477 creator A5016220289 @default.
- W3100565477 creator A5033474366 @default.
- W3100565477 creator A5036737482 @default.
- W3100565477 creator A5036793826 @default.
- W3100565477 creator A5071757529 @default.
- W3100565477 creator A5080978457 @default.
- W3100565477 date "2020-11-12" @default.
- W3100565477 modified "2023-10-07" @default.
- W3100565477 title "A comprehensive spectral assay library to quantify the Escherichia coli proteome by DIA/SWATH-MS" @default.
- W3100565477 cites W1483970101 @default.
- W3100565477 cites W1485263312 @default.
- W3100565477 cites W1533744928 @default.
- W3100565477 cites W1568072008 @default.
- W3100565477 cites W1946914101 @default.
- W3100565477 cites W1963898428 @default.
- W3100565477 cites W1969303200 @default.
- W3100565477 cites W2027222148 @default.
- W3100565477 cites W2039058260 @default.
- W3100565477 cites W2073371472 @default.
- W3100565477 cites W2073637473 @default.
- W3100565477 cites W2079843936 @default.
- W3100565477 cites W2089273345 @default.
- W3100565477 cites W2091377998 @default.
- W3100565477 cites W2091617667 @default.
- W3100565477 cites W2102572519 @default.
- W3100565477 cites W2105167594 @default.
- W3100565477 cites W2106376891 @default.
- W3100565477 cites W2108540012 @default.
- W3100565477 cites W2108699024 @default.
- W3100565477 cites W2112078820 @default.
- W3100565477 cites W2116063068 @default.
- W3100565477 cites W2116134054 @default.
- W3100565477 cites W2128890791 @default.
- W3100565477 cites W2129897014 @default.
- W3100565477 cites W2134001789 @default.
- W3100565477 cites W2135581618 @default.
- W3100565477 cites W2136861926 @default.
- W3100565477 cites W2138820253 @default.
- W3100565477 cites W2139578623 @default.
- W3100565477 cites W2141329441 @default.
- W3100565477 cites W2147189708 @default.
- W3100565477 cites W2163078532 @default.
- W3100565477 cites W2191824721 @default.
- W3100565477 cites W2200387749 @default.
- W3100565477 cites W2308104112 @default.
- W3100565477 cites W2341578253 @default.
- W3100565477 cites W2478869544 @default.
- W3100565477 cites W2488048472 @default.
- W3100565477 cites W2515644916 @default.
- W3100565477 cites W2519691041 @default.
- W3100565477 cites W2586637063 @default.
- W3100565477 cites W2587241659 @default.
- W3100565477 cites W2771144311 @default.
- W3100565477 cites W2805896072 @default.
- W3100565477 cites W2887294942 @default.
- W3100565477 cites W2890647322 @default.
- W3100565477 cites W2912715854 @default.
- W3100565477 cites W2914095857 @default.
- W3100565477 cites W2946442588 @default.
- W3100565477 cites W2950557897 @default.
- W3100565477 cites W2991580150 @default.
- W3100565477 cites W3005045932 @default.
- W3100565477 cites W3005683708 @default.
- W3100565477 cites W3005901855 @default.
- W3100565477 cites W3011306125 @default.
- W3100565477 cites W3013171490 @default.
- W3100565477 cites W3025831314 @default.
- W3100565477 cites W3093284294 @default.
- W3100565477 cites W3106135851 @default.
- W3100565477 cites W3136918052 @default.
- W3100565477 doi "https://doi.org/10.1038/s41597-020-00724-7" @default.
- W3100565477 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7665006" @default.
- W3100565477 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33184295" @default.
- W3100565477 hasPublicationYear "2020" @default.
- W3100565477 type Work @default.
- W3100565477 sameAs 3100565477 @default.
- W3100565477 citedByCount "24" @default.
- W3100565477 countsByYear W31005654772021 @default.
- W3100565477 countsByYear W31005654772022 @default.
- W3100565477 countsByYear W31005654772023 @default.
- W3100565477 crossrefType "journal-article" @default.
- W3100565477 hasAuthorship W3100565477A5001209740 @default.
- W3100565477 hasAuthorship W3100565477A5005287667 @default.
- W3100565477 hasAuthorship W3100565477A5010894905 @default.
- W3100565477 hasAuthorship W3100565477A5016220289 @default.
- W3100565477 hasAuthorship W3100565477A5033474366 @default.
- W3100565477 hasAuthorship W3100565477A5036737482 @default.
- W3100565477 hasAuthorship W3100565477A5036793826 @default.
- W3100565477 hasAuthorship W3100565477A5071757529 @default.
- W3100565477 hasAuthorship W3100565477A5080978457 @default.
- W3100565477 hasBestOaLocation W31005654771 @default.
- W3100565477 hasConcept C104317684 @default.
- W3100565477 hasConcept C104397665 @default.
- W3100565477 hasConcept C162356407 @default.
- W3100565477 hasConcept C185592680 @default.