FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100622014> ?p ?o ?g. }

• W3100622014 endingPage "3333" @default.
• W3100622014 startingPage "3322" @default.
• W3100622014 abstract "Protein dimerization systems controlled by red light with increased tissue penetration depth are a highly needed tool for clinical applications such as cell and gene therapies. However, mammalian applications of existing red light-induced dimerization systems are hampered by limitations of their two components: a photosensory protein (or photoreceptor) which often requires a mammalian exogenous chromophore and a naturally occurring photoreceptor binding protein typically having a complex structure and nonideal binding properties. Here, we introduce an efficient, generalizable method (COMBINES-LID) for creating highly specific, reversible light-induced heterodimerization systems independent of any existing binders to a photoreceptor. It involves a two-step binder screen (phage display and yeast two-hybrid) of a combinatorial nanobody library to obtain binders that selectively engage a light-activated form of a photoswitchable protein or domain not the dark form. Proof-of-principle was provided by engineering nanobody-based, red light-induced dimerization (nanoReD) systems comprising a truncated bacterial phytochrome sensory module using a mammalian endogenous chromophore, biliverdin, and light-form specific nanobodies. Selected nanoReD systems were biochemically characterized, exhibiting low dark activity and high induction specificity, and further demonstrated for the reversible control of protein translocation and activation of gene expression in mice. Overall, COMBINES-LID opens new opportunities for creating genetically encoded actuators for the optical manipulation of biological processes." @default.
• W3100622014 created "2020-11-23" @default.
• W3100622014 creator A5004226989 @default.
• W3100622014 creator A5006867970 @default.
• W3100622014 creator A5010085148 @default.
• W3100622014 creator A5017982475 @default.
• W3100622014 creator A5019281668 @default.
• W3100622014 creator A5044333513 @default.
• W3100622014 creator A5058941074 @default.
• W3100622014 creator A5080258929 @default.
• W3100622014 creator A5080808151 @default.
• W3100622014 creator A5082316109 @default.
• W3100622014 date "2020-11-12" @default.
• W3100622014 modified "2023-10-01" @default.
• W3100622014 title "Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity" @default.
• W3100622014 cites W1753684754 @default.
• W3100622014 cites W1903778810 @default.
• W3100622014 cites W1982409013 @default.
• W3100622014 cites W2001831734 @default.
• W3100622014 cites W2003885556 @default.
• W3100622014 cites W2011858837 @default.
• W3100622014 cites W2025021056 @default.
• W3100622014 cites W2025244200 @default.
• W3100622014 cites W2025395772 @default.
• W3100622014 cites W2029270622 @default.
• W3100622014 cites W2029852810 @default.
• W3100622014 cites W2031968879 @default.
• W3100622014 cites W2033447440 @default.
• W3100622014 cites W2050903472 @default.
• W3100622014 cites W2053356496 @default.
• W3100622014 cites W2057686161 @default.
• W3100622014 cites W2069893600 @default.
• W3100622014 cites W2083160307 @default.
• W3100622014 cites W2104579888 @default.
• W3100622014 cites W2130662176 @default.
• W3100622014 cites W2139823369 @default.
• W3100622014 cites W2161071729 @default.
• W3100622014 cites W2169610187 @default.
• W3100622014 cites W2487118445 @default.
• W3100622014 cites W2513345744 @default.
• W3100622014 cites W2601706957 @default.
• W3100622014 cites W2605999716 @default.
• W3100622014 cites W2734958598 @default.
• W3100622014 cites W2759860792 @default.
• W3100622014 cites W2766954609 @default.
• W3100622014 cites W2789732912 @default.
• W3100622014 cites W2891652830 @default.
• W3100622014 cites W2903602386 @default.
• W3100622014 cites W2955583983 @default.
• W3100622014 cites W2959505034 @default.
• W3100622014 cites W2970095718 @default.
• W3100622014 cites W3003212796 @default.
• W3100622014 cites W3008178969 @default.
• W3100622014 cites W4239525295 @default.
• W3100622014 doi "https://doi.org/10.1021/acssynbio.0c00397" @default.
• W3100622014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7749050" @default.
• W3100622014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33179507" @default.
• W3100622014 hasPublicationYear "2020" @default.
• W3100622014 type Work @default.
• W3100622014 sameAs 3100622014 @default.
• W3100622014 citedByCount "13" @default.
• W3100622014 countsByYear W31006220142021 @default.
• W3100622014 countsByYear W31006220142022 @default.
• W3100622014 countsByYear W31006220142023 @default.
• W3100622014 crossrefType "journal-article" @default.
• W3100622014 hasAuthorship W3100622014A5004226989 @default.
• W3100622014 hasAuthorship W3100622014A5006867970 @default.
• W3100622014 hasAuthorship W3100622014A5010085148 @default.
• W3100622014 hasAuthorship W3100622014A5017982475 @default.
• W3100622014 hasAuthorship W3100622014A5019281668 @default.
• W3100622014 hasAuthorship W3100622014A5044333513 @default.
• W3100622014 hasAuthorship W3100622014A5058941074 @default.
• W3100622014 hasAuthorship W3100622014A5080258929 @default.
• W3100622014 hasAuthorship W3100622014A5080808151 @default.
• W3100622014 hasAuthorship W3100622014A5082316109 @default.
• W3100622014 hasBestOaLocation W31006220142 @default.
• W3100622014 hasConcept C104317684 @default.
• W3100622014 hasConcept C12554922 @default.
• W3100622014 hasConcept C143065580 @default.
• W3100622014 hasConcept C147816474 @default.
• W3100622014 hasConcept C174851938 @default.
• W3100622014 hasConcept C178790620 @default.
• W3100622014 hasConcept C181199279 @default.
• W3100622014 hasConcept C185592680 @default.
• W3100622014 hasConcept C191908910 @default.
• W3100622014 hasConcept C192468462 @default.
• W3100622014 hasConcept C2776217839 @default.
• W3100622014 hasConcept C2776253728 @default.
• W3100622014 hasConcept C2779219270 @default.
• W3100622014 hasConcept C2986477067 @default.
• W3100622014 hasConcept C55493867 @default.
• W3100622014 hasConcept C59822182 @default.
• W3100622014 hasConcept C70721500 @default.
• W3100622014 hasConcept C86803240 @default.
• W3100622014 hasConcept C90180588 @default.
• W3100622014 hasConcept C9418097 @default.
• W3100622014 hasConcept C95444343 @default.
• W3100622014 hasConceptScore W3100622014C104317684 @default.

• next