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- W3100667750 abstract "Author(s): Boontanrart, Mandy | Advisor(s): Corn, Jacob E | Abstract: The fetal to adult hemoglobin switch is a developmental process by which fetal hemoglobin becomes silenced after birth and replaced by adult hemoglobin. Diseases caused by defective or missing adult hemoglobin, such as Sickle Cell Disease or β-Thalassemia, can be ameliorated by reactivating fetal hemoglobin. We discovered that knockdown or knockout of β-globin, a subunit of adult hemoglobin, led to robust upregulation of γ-globin, a subunit of fetal hemoglobin. This phenomenon suggested that red blood cells have an inherent ability to upregulate fetal hemoglobin in the event that adult hemoglobin is lacking.We developed multiple gene-editing tools in an immortalized erythroid cell model to investigate the molecular mechanisms behind the increase in fetal hemoglobin. Time-course transcriptomics identified ATF4, a transcription factor, as a causal regulator of this response. Further analysis also converged upon downregulation of MYB and BCL11A, known repressors of γ-globin, described in detail in chapter 2. Further work in chapter 3 explores other possible fetal hemoglobin regulators as discovered by CRISPRi arrayed mediated knockdown experiments. This work furthers our understanding of fundamental mechanisms of gene regulation and how cellular and molecular events influence red blood cell differentiation." @default.
- W3100667750 created "2020-11-23" @default.
- W3100667750 creator A5026303505 @default.
- W3100667750 date "2020-01-01" @default.
- W3100667750 modified "2023-09-26" @default.
- W3100667750 title "Flipping the Hemoglobin Switch and Discovering Regulators Involved in Fetal Hemoglobin Reactivation" @default.
- W3100667750 cites W1593588462 @default.
- W3100667750 cites W1881664604 @default.
- W3100667750 cites W1968165696 @default.
- W3100667750 cites W1968286431 @default.
- W3100667750 cites W1972387348 @default.
- W3100667750 cites W1974127892 @default.
- W3100667750 cites W1974742081 @default.
- W3100667750 cites W1978510012 @default.
- W3100667750 cites W1980697072 @default.
- W3100667750 cites W1988866031 @default.
- W3100667750 cites W1990599619 @default.
- W3100667750 cites W1990738826 @default.
- W3100667750 cites W1998075386 @default.
- W3100667750 cites W2001898141 @default.
- W3100667750 cites W2007713145 @default.
- W3100667750 cites W2007823610 @default.
- W3100667750 cites W2008051478 @default.
- W3100667750 cites W2008989169 @default.
- W3100667750 cites W2011228037 @default.
- W3100667750 cites W2012020901 @default.
- W3100667750 cites W2012382858 @default.
- W3100667750 cites W2019425923 @default.
- W3100667750 cites W2021341670 @default.
- W3100667750 cites W2028798136 @default.
- W3100667750 cites W2034397996 @default.
- W3100667750 cites W2037238966 @default.
- W3100667750 cites W2038881587 @default.
- W3100667750 cites W2040981306 @default.
- W3100667750 cites W2041995719 @default.
- W3100667750 cites W2043362808 @default.
- W3100667750 cites W2049129790 @default.
- W3100667750 cites W2053593146 @default.
- W3100667750 cites W2061050681 @default.
- W3100667750 cites W2067348966 @default.
- W3100667750 cites W2069646782 @default.
- W3100667750 cites W2070879341 @default.
- W3100667750 cites W2089894746 @default.
- W3100667750 cites W2093507774 @default.
- W3100667750 cites W2101901913 @default.
- W3100667750 cites W2102324931 @default.
- W3100667750 cites W2119499340 @default.
- W3100667750 cites W2121659981 @default.
- W3100667750 cites W2121759181 @default.
- W3100667750 cites W2124514674 @default.
- W3100667750 cites W2128016314 @default.
- W3100667750 cites W2131527797 @default.
- W3100667750 cites W2133464522 @default.
- W3100667750 cites W2134667612 @default.
- W3100667750 cites W2140952049 @default.
- W3100667750 cites W2152505144 @default.
- W3100667750 cites W2153926593 @default.
- W3100667750 cites W2156615060 @default.
- W3100667750 cites W2157830903 @default.
- W3100667750 cites W2157952888 @default.
- W3100667750 cites W2158217645 @default.
- W3100667750 cites W2160051211 @default.
- W3100667750 cites W2161143576 @default.
- W3100667750 cites W2162143298 @default.
- W3100667750 cites W2162356875 @default.
- W3100667750 cites W2168488235 @default.
- W3100667750 cites W2170830174 @default.
- W3100667750 cites W2171702968 @default.
- W3100667750 cites W2176406552 @default.
- W3100667750 cites W2214069605 @default.
- W3100667750 cites W2259938310 @default.
- W3100667750 cites W2332195908 @default.
- W3100667750 cites W2419968394 @default.
- W3100667750 cites W2504899707 @default.
- W3100667750 cites W2523099503 @default.
- W3100667750 cites W2523751195 @default.
- W3100667750 cites W2530601336 @default.
- W3100667750 cites W2603331580 @default.
- W3100667750 cites W2609281653 @default.
- W3100667750 cites W2610584680 @default.
- W3100667750 cites W2620721366 @default.
- W3100667750 cites W2732009893 @default.
- W3100667750 cites W2762440871 @default.
- W3100667750 cites W2766599608 @default.
- W3100667750 cites W2793309516 @default.
- W3100667750 cites W2794952277 @default.
- W3100667750 cites W2795328021 @default.
- W3100667750 cites W2801952734 @default.
- W3100667750 cites W2803271184 @default.
- W3100667750 cites W2884535700 @default.
- W3100667750 cites W2886819502 @default.
- W3100667750 cites W2888651633 @default.
- W3100667750 cites W2895553720 @default.
- W3100667750 cites W2897295193 @default.
- W3100667750 cites W2900885456 @default.
- W3100667750 cites W2910850705 @default.
- W3100667750 cites W2923636118 @default.
- W3100667750 cites W2924385776 @default.
- W3100667750 cites W2934310150 @default.
- W3100667750 cites W2942563094 @default.